Abstract:
:X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents. Here we have examined at single-bond resolution the interactions of three commonly used nucleases (DNAase I, DNAase II, and copper-phenanthroline) with a DNA of natural origin, the 160 bp tyrT promoter. All three of these reagents seem sensitive to DNA backbone geometry rather than base sequence per se. Their sequence-dependent patterns of cleavage provide evidence for structural polymorphism of several sorts: global variation in helix groove width, global variation in radial asymmetry, and local variation in phosphate accessibility. These findings explain how sequence zones of a certain base composition, or purine-pyrimidine asymmetry, can influence the recognition of DNA by protein molecules.
journal_name
Celljournal_title
Cellauthors
Drew HR,Travers AAdoi
10.1016/0092-8674(84)90379-9subject
Has Abstractpub_date
1984-06-01 00:00:00pages
491-502issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(84)90379-9journal_volume
37pub_type
杂志文章相关文献
CELL文献大全abstract::Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperon...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00351-9
更新日期:2003-05-30 00:00:00
abstract::We used clinical tissue from lethal metastatic castration-resistant prostate cancer (CRPC) patients obtained at rapid autopsy to evaluate diverse genomic, transcriptomic, and phosphoproteomic datasets for pathway analysis. Using Tied Diffusion through Interacting Events (TieDIE), we integrated differentially expressed...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.07.007
更新日期:2016-08-11 00:00:00
abstract::We have shown that covalent conjugation of ubiquitin to proteins is temperature-sensitive in the mouse cell cycle mutant ts85 due to a specifically thermolabile ubiquitin-activating enzyme (accompanying paper). We show here that degradation of short-lived proteins is also temperature sensitive in ts85 , in contrast to...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90300-3
更新日期:1984-05-01 00:00:00
abstract::CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at a hi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80240-8
更新日期:1997-05-16 00:00:00
abstract::The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90150-b
更新日期:1992-02-21 00:00:00
abstract::In contrast to uninfected chicken embryo fibroblasts (CEFs), CEFs infected with a retroviral vector that carries the v-erbA gene of avian erythroblastosis virus displayed new properties. These included limited anchorage-independent growth in soft agar, growth without latency in serum-supplemented medium, ability to ov...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90545-9
更新日期:1987-06-05 00:00:00
abstract::Chemotherapy results in a frequent yet poorly understood syndrome of long-term neurological deficits. Neural precursor cell dysfunction and white matter dysfunction are thought to contribute to this debilitating syndrome. Here, we demonstrate persistent depletion of oligodendrocyte lineage cells in humans who received...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.10.049
更新日期:2019-01-10 00:00:00
abstract::Vertebrate Hoxd genes are sequentially activated during the morphogenesis and pattern formation of the limb. Using the approach of gene disruption via homologous recombination in embryonic stem cells, we have assessed the function of the last gene of the complex, Hoxd-13. Mutant mice displayed skeletal alterations alo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90378-4
更新日期:1993-11-05 00:00:00
abstract::Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00435-4
更新日期:2001-07-27 00:00:00
abstract::Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.10.008
更新日期:2015-11-05 00:00:00
abstract::Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.09.013
更新日期:2011-09-30 00:00:00
abstract::We have previously cloned lethal(2)giant larvae, a tumor-suppressor gene of Drosophila that normally controls cell proliferation and/or differentiation in the optic centers of the brain and the imaginal discs. Here we describe the structure of the l(2)gl genes as determined by sequencing genomic and cDNA clones. The s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90217-0
更新日期:1987-07-17 00:00:00
abstract::Many organs are composed of epithelial tubes that transport vital fluids. Such tubular organs develop in many different ways and generate tubes of widely varying sizes and structures, but always with the apical epithelial surface lining the lumen. We describe recent progress in several diverse cell culture and genetic...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)01283-7
更新日期:2003-01-10 00:00:00
abstract::Lambda integrative recombination depends on supercoiling of the phage attachment site, attP. Using dimethylsulfate protection and indirect end-labeling, the interaction of the recombination proteins Int and IHF with supercoiled and linear attP has been studied. Supercoiling enhances the binding of Int to attP, but not...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90700-2
更新日期:1986-09-26 00:00:00
abstract::In prokaryotes, the toxin-antitoxin systems are thought to play important roles in growth regulation under stress conditions. In the E. coli MazE-MazF system, MazF toxin functions as an mRNA interferase cleaving mRNAs at ACA sequences to inhibit protein synthesis leading to cell growth arrest. Myxococcus xanthus is a ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.11.044
更新日期:2008-01-11 00:00:00
abstract::The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90095-8
更新日期:1983-12-01 00:00:00
abstract::As these paired Commentaries discuss, neuroscientists and architects are just beginning to collaborate, each bringing what they know about their respective fields to the task of improving the environment of research buildings and laboratories. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.10.012
更新日期:2006-10-20 00:00:00
abstract::Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.12.020
更新日期:2013-01-31 00:00:00
abstract::Protein tethers can bridge gaps between membranes. Ren et al. (2009) now provide evidence that the yeast Dsl1 complex tethers vesicles to the endoplasmic reticulum (ER) by binding ER SNARE proteins at its base and capturing vesicles using a loop region that extends 20 nm from the ER membrane. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2009.11.041
更新日期:2009-12-11 00:00:00
abstract::Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90222-7
更新日期:1977-02-01 00:00:00
abstract::The Ion gene of E. coli controls the stability of two bacteriophage lambda proteins. The functional half-life of the phage N gene product, measured by complementation, is increased about 5-fold in Ion mutant strains, from 2 min to 10 min. The chemical half-life of N protein, determined by its disappearance on polyacry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90518-3
更新日期:1981-04-01 00:00:00
abstract::The membrane fusion potential of influenza HA, like many viral membrane-fusion glycoproteins, is generated by proteolytic cleavage of a biosynthetic precursor. The three-dimensional structure of ectodomain of the precursor HA0 has been determined and compared with that of cleaved HA. The cleavage site is a prominent s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81771-7
更新日期:1998-10-30 00:00:00
abstract::Staphylococcal enterotoxin B is known to be a powerful T cell stimulant in mouse and man. In this paper we show that, for mice, this is because the protein in association with major histocompatibility complex class II molecules stimulates virtually all T cells bearing V beta 3 and V beta 8.1, 8.2, and 8.3, and few oth...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90980-x
更新日期:1989-01-13 00:00:00
abstract::Transcription termination by bacterial RNA polymerase (RNAP) occurs at sequences coding for a GC-rich RNA hairpin followed by a U-rich tract. We used single-molecule techniques to investigate the mechanism by which three representative terminators (his, t500, and tR2) destabilize the elongation complex (EC). For his a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.01.027
更新日期:2008-03-21 00:00:00
abstract::Unique loci in the human genome were examined with restriction enzymes in order to detect restriction fragment length polymorphisms (RFLPs). Of 31 arbitrary loci, nine were detectably polymorphic, reflecting ten polymorphic restriction sites. Nine of the ten polymorphic sites were revealed with two restriction enzymes...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90081-3
更新日期:1984-01-01 00:00:00
abstract::GAL4 derivatives containing an activation domain alleviated repression of a promoter during nucleosome assembly. A GAL4 derivative lacking an activation domain stably bound the promoter during nucleosome assembly but was not sufficient to preserve promoter function. The activation domain of GAL4 derivatives was essent...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90237-s
更新日期:1991-02-08 00:00:00
abstract::The membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ACE2) is an essential regulator of heart function. Now, Li at al. identify and characterize an unexpected second function of ACE2 as a partner of the SARS-CoV spike glycoprotein in mediating virus entry and cell fusion. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00976-0
更新日期:2003-12-12 00:00:00
abstract::Xenopus laevis oocytes respond to high temperature (greater than 31 degrees C) by the synthesis of one major (70 kilodalton) protein and by a gradual reduction in the rate of normal protein synthesis. In contrast with most other cells, the heat-shock response of Xenopus oocytes is controlled exclusively at the transla...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90443-3
更新日期:1982-07-01 00:00:00
abstract::Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90401-9
更新日期:1982-11-01 00:00:00
abstract::This year, the Lasker Foundation confers its Clinical Medical Research Award on Akira Endo for his isolation from fungi of statins, potent inhibitors of cholesterol synthesis in the liver. The introduction of statins to clinical practice has markedly reduced morbidity and mortality from atherosclerotic cardiovascular ...
journal_title:Cell
pub_type: 历史文章,杂志文章
doi:10.1016/j.cell.2008.09.008
更新日期:2008-09-19 00:00:00