Abstract:
:Lambda integrative recombination depends on supercoiling of the phage attachment site, attP. Using dimethylsulfate protection and indirect end-labeling, the interaction of the recombination proteins Int and IHF with supercoiled and linear attP has been studied. Supercoiling enhances the binding of Int to attP, but not if a truncated attP site is employed or if IHF is omitted. We reason that the altered affinity reflects the formation of a higher-order nucleoprotein structure, an "attP intasome," that involves Int and IHF assembly of both arms of attP into a wrapped configuration. The good correlation between the degree and sign of supercoiling needed to promote recombination and that needed for the "attP intasome" indicates that the primary role of supercoiling is to drive the formation of the wrapped structure.
journal_name
Celljournal_title
Cellauthors
Richet E,Abcarian P,Nash HAdoi
10.1016/0092-8674(86)90700-2subject
Has Abstractpub_date
1986-09-26 00:00:00pages
1011-21issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(86)90700-2journal_volume
46pub_type
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