当前位置： SCI文献检索 > CELL期刊下所有文献 > Functional analysis of a growth factor-responsive transcription factor complex.

Functional analysis of a growth factor-responsive transcription factor complex.

Abstract：

:Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a ternary complex with SRF. Simultaneous expression of these SRF and Elk-1 derivatives restores serum-regulated activity to the mutated SRE in transfected cells. Efficient transcriptional activation is dependent on the regulated phosphorylation of Elk-1 C-terminal MAP kinase sites and requires the C-terminal sequences of SRF as well as SRF sequences that mediate ternary complex formation. These experiments provide direct evidence that SRF and Elk-1 functionally cooperate in the ternary complex at the SRE to regulate transcription.

journal_name

Cell

journal_title

Cell

authors

Hill CS,Marais R,John S,Wynne J,Dalton S,Treisman R

doi

10.1016/0092-8674(93)90238-l

subject

Has Abstract

pub_date

1993-04-23 00:00:00

pages

395-406

issue

2

eissn

0092-8674

issn

1097-4172

pii

0092-8674(93)90238-L

journal_volume

73

pub_type

杂志文章

相关文献

CELL文献大全
  • Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion.

    abstract：:In Drosophila, the fat body (FB), a functional analog of the vertebrate adipose tissue, is the nutrient sensor that conveys the nutrient status to the insulin-producing cells (IPCs) in the fly brain to release Drosophila insulin-like peptides (Dilps). Dilp secretion in turn regulates energy balance and promotes system...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2012.08.019

    authors： Rajan A,Perrimon N

    更新日期：2012-09-28 00:00:00

  • Oncogenic activity of the c-Myc protein requires dimerization with Max.

    abstract：:c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(93)90663-b

    authors： Amati B,Brooks MW,Levy N,Littlewood TD,Evan GI,Land H

    更新日期：1993-01-29 00:00:00

  • A Scientist and a Journalist Walk into a Bar….

    abstract：:Who are science journalists, and how can journalists and research scientists work together to improve science communication? ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2016.10.051

    authors： Matheson S

    更新日期：2016-11-17 00:00:00

  • Crosstalk between muscularis macrophages and enteric neurons regulates gastrointestinal motility.

    abstract：:Intestinal peristalsis is a dynamic physiologic process influenced by dietary and microbial changes. It is tightly regulated by complex cellular interactions; however, our understanding of these controls is incomplete. A distinct population of macrophages is distributed in the intestinal muscularis externa. We demonst...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.04.050

    authors： Muller PA,Koscsó B,Rajani GM,Stevanovic K,Berres ML,Hashimoto D,Mortha A,Leboeuf M,Li XM,Mucida D,Stanley ER,Dahan S,Margolis KG,Gershon MD,Merad M,Bogunovic M

    更新日期：2014-07-17 00:00:00

  • Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.

    abstract：:Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin r...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.09.008

    authors： Kschonsak M,Merkel F,Bisht S,Metz J,Rybin V,Hassler M,Haering CH

    更新日期：2017-10-19 00:00:00

  • Evolutionary history and adaptation from high-coverage whole-genome sequences of diverse African hunter-gatherers.

    abstract：:To reconstruct modern human evolutionary history and identify loci that have shaped hunter-gatherer adaptation, we sequenced the whole genomes of five individuals in each of three different hunter-gatherer populations at > 60× coverage: Pygmies from Cameroon and Khoesan-speaking Hadza and Sandawe from Tanzania. We ide...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2012.07.009

    authors： Lachance J,Vernot B,Elbers CC,Ferwerda B,Froment A,Bodo JM,Lema G,Fu W,Nyambo TB,Rebbeck TR,Zhang K,Akey JM,Tishkoff SA

    更新日期：2012-08-03 00:00:00

  • Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p.

    abstract：:We have reproduced the posttranslational mode of protein translocation across the endoplasmic reticulum membrane with reconstituted proteoliposomes containing a purified complex of seven yeast proteins. This Sec complex includes a heterotrimeric Sec61p complex, homologous to that in mammals, as well as all other membr...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(95)90077-2

    authors： Panzner S,Dreier L,Hartmann E,Kostka S,Rapoport TA

    更新日期：1995-05-19 00:00:00

  • Linking retroelements to autoimmunity.

    abstract：:In this issue, Stetson et al. (2008) report a mechanism by which host cells avert an autoimmune response to self-nucleic acids. They show that the nuclease Trex1 prevents the accumulation of DNA derived from endogenous retroelements that, if left unchecked, trigger elevated production of type I interferons leading to ...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2008.08.010

    authors： Bhoj VG,Chen ZJ

    更新日期：2008-08-22 00:00:00

  • Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.

    abstract：:We mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (neor) gene was introduced into an exon of a cloned fragment of the Hprt gene and used to transfect ES cells. Among the G418r col...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(87)90646-5

    authors： Thomas KR,Capecchi MR

    更新日期：1987-11-06 00:00:00

  • Enhancer activation requires trans-recruitment of a mega transcription factor complex.

    abstract：:Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.08.027

    authors： Liu Z,Merkurjev D,Yang F,Li W,Oh S,Friedman MJ,Song X,Zhang F,Ma Q,Ohgi KA,Krones A,Rosenfeld MG

    更新日期：2014-10-09 00:00:00

  • DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.

    abstract：:DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2)...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(85)90150-3

    authors： Bohr VA,Smith CA,Okumoto DS,Hanawalt PC

    更新日期：1985-02-01 00:00:00

  • Calmodulin-induced early-onset diabetes in transgenic mice.

    abstract：:Calmodulin is implicated as the primary transducer of the calcium signal in pancreatic beta cells, where it is present at very high concentrations. We have produced three lines of transgenic mice carrying a calmodulin minigene regulated by the rat insulin II promoter. Immunohistochemistry and hybridization analyses in...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(89)90505-9

    authors： Epstein PN,Overbeek PA,Means AR

    更新日期：1989-09-22 00:00:00

  • Electron microscopic visualization of recA-DNA filaments: evidence for a cyclic extension of duplex DNA.

    abstract：:As visualized by electron microscopy, RecA protein binds in a highly cooperative manner to single-stranded fd DNA in solutions of 0.01 M Tris (pH 7.5). The resulting nucleoprotein filament loops are 1.25 micrometers in length, have a fiber diameter of 12 nm and show an indication of a 4.5 nm repeat along the axis of t...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90055-1

    authors： Dunn K,Chrysogelos S,Griffith J

    更新日期：1982-04-01 00:00:00

  • Drug-Driven Phenotypic Convergence Supports Rational Treatment Strategies of Chronic Infections.

    abstract：:Chronic Pseudomonas aeruginosa infections evade antibiotic therapy and are associated with mortality in cystic fibrosis (CF) patients. We find that in vitro resistance evolution of P. aeruginosa toward clinically relevant antibiotics leads to phenotypic convergence toward distinct states. These states are associated w...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.12.012

    authors： Imamovic L,Ellabaan MMH,Dantas Machado AM,Citterio L,Wulff T,Molin S,Krogh Johansen H,Sommer MOA

    更新日期：2018-01-11 00:00:00

  • A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligation.

    abstract：:We have constructed an actin-HIS4 gene fusion, such that expression of HIS4 requires proper splicing of the actin intron. Using this chimeric gene in an in vivo screen for splicing mutations, we have isolated a G to A transition in the fifth position of the yeast 5' consensus sequence/GTAPyGT. This mutation still allo...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(85)90065-0

    authors： Parker R,Guthrie C

    更新日期：1985-05-01 00:00:00

  • Alternative splicing accounts for the four forms of myelin basic protein.

    abstract：:We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(85)90245-4

    authors： de Ferra F,Engh H,Hudson L,Kamholz J,Puckett C,Molineaux S,Lazzarini RA

    更新日期：1985-12-01 00:00:00

  • Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.

    abstract：:Many cofactors bind the hormone-activated estrogen receptor (ER), yet the specific regulators of endogenous ER-mediated gene transcription are unknown. Using chromatin immunoprecipitation (ChIP), we find that ER and a number of coactivators rapidly associate with estrogen responsive promoters following estrogen treatm...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)00188-4

    authors： Shang Y,Hu X,DiRenzo J,Lazar MA,Brown M

    更新日期：2000-12-08 00:00:00

  • Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.

    abstract：:We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(84)90225-3

    authors： Malissen B,Price MP,Goverman JM,McMillan M,White J,Kappler J,Marrack P,Pierres A,Pierres M,Hood L

    更新日期：1984-02-01 00:00:00

  • Factor-independent termination of transcription in a stretch of deoxyadenosine residues in the template DNA.

    abstract：:The primer transcript of plasmid ColE1 extends beyond the replication origin in either of two different modes. It does or does not form a hybrid with the template DNA. When a stretch of 20 deoxyadenosine residues is inserted into the template strand downstream of the origin, more than 90% of hybridized transcripts and...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(87)90131-0

    authors： Tomizawa J,Masukata H

    更新日期：1987-11-20 00:00:00

  • Global proteomic assessment of the classical protein-tyrosine phosphatome and "Redoxome".

    abstract：:Protein-tyrosine phosphatases (PTPs), along with protein-tyrosine kinases, play key roles in cellular signaling. All Class I PTPs contain an essential active site cysteinyl residue, which executes a nucleophilic attack on substrate phosphotyrosyl residues. The high reactivity of the catalytic cysteine also predisposes...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2011.07.020

    authors： Karisch R,Fernandez M,Taylor P,Virtanen C,St-Germain JR,Jin LL,Harris IS,Mori J,Mak TW,Senis YA,Östman A,Moran MF,Neel BG

    更新日期：2011-09-02 00:00:00

  • Secretin-Activated Brown Fat Mediates Prandial Thermogenesis to Induce Satiation.

    abstract：:The molecular mediator and functional significance of meal-associated brown fat (BAT) thermogenesis remains elusive. Here, we identified the gut hormone secretin as a non-sympathetic BAT activator mediating prandial thermogenesis, which consequentially induces satiation, thereby establishing a gut-secretin-BAT-brain a...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2018.10.016

    authors： Li Y,Schnabl K,Gabler SM,Willershäuser M,Reber J,Karlas A,Laurila S,Lahesmaa M,U Din M,Bast-Habersbrunner A,Virtanen KA,Fromme T,Bolze F,O'Farrell LS,Alsina-Fernandez J,Coskun T,Ntziachristos V,Nuutila P,Klingenspor M

    更新日期：2018-11-29 00:00:00

  • Selective Chemical Inhibition of PGC-1α Gluconeogenic Activity Ameliorates Type 2 Diabetes.

    abstract：:Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.03.001

    authors： Sharabi K,Lin H,Tavares CDJ,Dominy JE,Camporez JP,Perry RJ,Schilling R,Rines AK,Lee J,Hickey M,Bennion M,Palmer M,Nag PP,Bittker JA,Perez J,Jedrychowski MP,Ozcan U,Gygi SP,Kamenecka TM,Shulman GI,Schreiber SL,Gr

    更新日期：2017-03-23 00:00:00

  • All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1.

    abstract：:Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory proce...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.01.001

    authors： Fan LZ,Kheifets S,Böhm UL,Wu H,Piatkevich KD,Xie ME,Parot V,Ha Y,Evans KE,Boyden ES,Takesian AE,Cohen AE

    更新日期：2020-02-06 00:00:00

  • Protein kinase C phosphorylation at Thr 654 of the unoccupied EGF receptor and EGF binding regulate functional receptor loss by independent mechanisms.

    abstract：:To test the functional consequence of phosphorylation of the EGF receptor at Thr 654 by protein kinase C, the normal Thr 654 human EGF receptor cDNA or a mutant encoding an Ala 654 were expressed in heterologous cells. In cell lines expressing both the Thr 654 and Ala 654 receptors, functional cell-surface Thr 654 rec...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(86)90006-1

    authors： Lin CR,Chen WS,Lazar CS,Carpenter CD,Gill GN,Evans RM,Rosenfeld MG

    更新日期：1986-03-28 00:00:00

  • The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors.

    abstract：:Axonal transport has been intensively examined as a good model for studying the mechanism of organelle transport in cells, but it is still unclear how different types of membrane organelles are transported through the nerve axon. To elucidate the function of this mechanism, we have cloned KIF1A, a novel neuron-specifi...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(95)90538-3

    authors： Okada Y,Yamazaki H,Sekine-Aizawa Y,Hirokawa N

    更新日期：1995-06-02 00:00:00

  • Paracrine signaling through the JAK/STAT pathway activates invasive behavior of ovarian epithelial cells in Drosophila.

    abstract：:The JAK/STAT signaling pathway, renowned for its effects on cell proliferation and survival, is constitutively active in various human cancers, including ovarian. We have found that JAK and STAT are required to convert the border cells in the Drosophila ovary from stationary, epithelial cells to migratory, invasive ce...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(01)00607-9

    authors： Silver DL,Montell DJ

    更新日期：2001-12-28 00:00:00

  • DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.

    abstract：:We studied the chromatin structure and methylation of ribosomal RNA genes (rDNA) in hybrids between Xenopus laevis and Xenopus borealis. S1-nuclease protection experiments showed that 97%-98% of the rRNA precursor in hybrid tadpoles was of the X. laevis type. Preferential expression of the laevis rDNA was correlated w...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90105-2

    authors： Macleod D,Bird A

    更新日期：1982-05-01 00:00:00

  • Structural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function.

    abstract：:Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular a...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2009.10.010

    authors： Byeon IJ,Meng X,Jung J,Zhao G,Yang R,Ahn J,Shi J,Concel J,Aiken C,Zhang P,Gronenborn AM

    更新日期：2009-11-13 00:00:00

  • Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity.

    abstract：:Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on t...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81615-3

    authors： Sen J,Goltz JS,Stevens L,Stein D

    更新日期：1998-11-13 00:00:00

  • Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene.

    abstract：:In cells of the B-lymphocyte lineage, 8.4 kb transcripts are constitutively produced from unrearranged kappa constant region (kappa 0) loci. To help elucidate the molecular basis of this phenomenon, we have determined the nucleotide sequence surrounding the site of transcriptional initiation. The kappa 0 transcripts a...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90401-3

    authors： Van Ness BG,Weigert M,Coleclough C,Mather EL,Kelley DE,Perry RP

    更新日期：1981-12-01 00:00:00