Abstract：
:We have reproduced the posttranslational mode of protein translocation across the endoplasmic reticulum membrane with reconstituted proteoliposomes containing a purified complex of seven yeast proteins. This Sec complex includes a heterotrimeric Sec61p complex, homologous to that in mammals, as well as all other membrane proteins found in genetic screens for translocation components. Efficient posttranslational translocation also requires the addition of lumenal Kar2p (BiP) and ATP. The trimeric Sec61p complex also exists as a separate entity that, in contrast with the large Sec complex, is associated with membrane-bound ribosomes. We therefore hypothesize that distinct membrane protein complexes function in co- and posttranslational translocation pathways.
journal_name
Celljournal_title
Cellauthors
Panzner S,Dreier L,Hartmann E,Kostka S,Rapoport TAdoi
10.1016/0092-8674(95)90077-2subject
Has Abstractpub_date
1995-05-19 00:00:00pages
561-70issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(95)90077-2journal_volume
81pub_type
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