Abstract:
:Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and protists (including important human pathogens like Plasmodium)-suggests that many eukaryotic organisms share a common gamete fusion mechanism. Here, we report combined bioinformatic, biochemical, mutational, and X-ray crystallographic studies on the unicellular alga Chlamydomonas reinhardtii HAP2 that reveal homology to class II viral membrane fusion proteins. We further show that targeting the segment corresponding to the fusion loop by mutagenesis or by antibodies blocks gamete fusion. These results demonstrate that HAP2 is the gamete fusogen and suggest a mechanism of action akin to viral fusion, indicating a way to block Plasmodium transmission and highlighting the impact of virus-cell genetic exchanges on the evolution of eukaryotic life.
journal_name
Celljournal_title
Cellauthors
Fédry J,Liu Y,Péhau-Arnaudet G,Pei J,Li W,Tortorici MA,Traincard F,Meola A,Bricogne G,Grishin NV,Snell WJ,Rey FA,Krey Tdoi
10.1016/j.cell.2017.01.024subject
Has Abstractpub_date
2017-02-23 00:00:00pages
904-915.e10issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(17)30109-5journal_volume
168pub_type
杂志文章相关文献
CELL文献大全abstract::A key step in the regulation of transcription involves interactions between promoter-selective factors and various components of the transcriptional apparatus. Here we report the requirements for transcriptional activation directed by NTF-1, a developmentally regulated transcription factor in Drosophila. Reconstituted...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90019-2
更新日期:1991-08-09 00:00:00
abstract::Protein function is often regulated by posttranslational modifications (PTMs), and recent advances in mass spectrometry have resulted in an exponential increase in PTM identification. However, the functional significance of the vast majority of these modifications remains unknown. To address this problem, we compiled ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.05.036
更新日期:2012-07-20 00:00:00
abstract::The preprotein translocase of the outer membrane of mitochondria (TOM complex) facilitates the recognition, insertion, and translocation of nuclear-encoded mitochondrial preproteins. We have purified the TOM complex from Neurospora crassa and analyzed its composition and functional properties. The TOM complex contains...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81206-4
更新日期:1998-06-12 00:00:00
abstract::An injection of aphidicolin into early Drosophila embryos inhibits DNA synthesis and nuclear division, while centrosome replication and many other aspects of the mitotic cycle continue. If aphidicolin is injected at nuclear cycle 7-8, the normal migration of nuclei to the embryo cortex is completely inhibited. In most...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90130-x
更新日期:1989-05-19 00:00:00
abstract::Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00817-6
更新日期:2002-07-12 00:00:00
abstract::Increased protein synthesis plays an etiologic role in diverse cancers. Here, we demonstrate that METTL13 (methyltransferase-like 13) dimethylation of eEF1A (eukaryotic elongation factor 1A) lysine 55 (eEF1AK55me2) is utilized by Ras-driven cancers to increase translational output and promote tumorigenesis in vivo. ME...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.038
更新日期:2019-01-24 00:00:00
abstract::Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00007-6
更新日期:2000-07-07 00:00:00
abstract::Posttranslational modifications control microtubule behavior, yet assigning roles to particular signals was hampered by lack of defined in vitro systems. In this issue of Cell, Valenstein and Roll-Mecak establish a biochemical platform to interrogate consequences of microtubule polyglutamylation, thereby providing imp...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2016.02.016
更新日期:2016-02-25 00:00:00
abstract::The sites at which topoisomerase I interacts with the transcriptionally active ribosomal chromatin of Xenopus oocytes were mapped by treating oocyte nuclei first with camptothecin to stabilize topoisomerase I-rDNA reaction intermediates, and then with SDS to resolve these adducts as protein-linked nicks in the DNA. St...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90471-0
更新日期:1988-02-26 00:00:00
abstract::The V(D)J recombinase subunits Rag-1 and Rag-2 mediate assembly of antigen receptor gene segments. We studied the mechanisms of DNA recognition by Rag-1/Rag-2 using surface plasmon resonance. The critical step for signal recognition is binding of Rag-1 to the nonamer. This is achieved by a region of Rag-1 homologous t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81344-6
更新日期:1996-10-18 00:00:00
abstract::The P7C3 class of aminopropyl carbazole chemicals fosters the survival of neurons in a variety of rodent models of neurodegeneration or nerve cell injury. To uncover its mechanism of action, an active derivative of P7C3 was modified to contain both a benzophenone for photocrosslinking and an alkyne for CLICK chemistry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.07.040
更新日期:2014-09-11 00:00:00
abstract::Receptor-interacting protein kinase (RIPK)-1 is involved in RIPK3-dependent and -independent signaling pathways leading to cell death and/or inflammation. Genetic ablation of ripk1 causes postnatal lethality, which was not prevented by deletion of ripk3, caspase-8, or fadd. However, animals that lack RIPK1, RIPK3, and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.04.018
更新日期:2014-05-22 00:00:00
abstract::Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90015-7
更新日期:1985-11-01 00:00:00
abstract::Synaptotagmin is an abundant synaptic vesicle-associated protein proposed to be involved in calcium-mediated neurotransmitter release. Our molecular and genetic results demonstrate that, although synaptotagmin is required for the proper function of the presynaptic nerve terminal in C. elegans, some neurotransmitter re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90357-v
更新日期:1993-07-02 00:00:00
abstract::p11, through unknown mechanisms, is required for behavioral and cellular responses to selective serotonin reuptake inhibitors (SSRIs). We show that SMARCA3, a chromatin-remodeling factor, is a target for the p11/annexin A2 heterotetrameric complex. Determination of the crystal structure indicates that SMARCA3 peptide ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.01.014
更新日期:2013-02-14 00:00:00
abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.014
更新日期:2015-06-04 00:00:00
abstract::Maintaining the pluripotency of mouse ES cells requires both LIF (leukemia inhibitory factor) and unknown factors in serum. The paper from Ying et al. in this issue of Cell shows that BMP (bone morphogenetic protein) can replace serum in this capacity, defining molecular requirements for ES cell self-renewal. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/s0092-8674(03)00853-5
更新日期:2003-10-31 00:00:00
abstract::We have investigated whether histone H1 is present on active Balbiani ring genes in the salivary glands of Chironomus tentans using immunoelectron microscopy. The genes were studied in two activity states: maximally activated genes with a fully extended template and repressed genes in a 30 nm fiber conformation. Histo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90717-s
更新日期:1990-01-12 00:00:00
abstract::Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.04.033
更新日期:2017-05-18 00:00:00
abstract::In causing disease, pathogens outmaneuver host defenses through a dedicated arsenal of virulence determinants that specifically bind or modify individual host molecules. This dedication limits the intruder to a defined range of hosts. Newly emerging diseases mostly involve existing pathogens whose arsenal has been alt...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.03.049
更新日期:2007-06-01 00:00:00
abstract::Computational modeling and the theory of nonlinear dynamical systems allow one to not simply describe the events of the cell cycle, but also to understand why these events occur, just as the theory of gravitation allows one to understand why cannonballs fly in parabolic arcs. The simplest examples of the eukaryotic ce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.03.006
更新日期:2011-03-18 00:00:00
abstract::A major problem in treatment of cancers arising in steroid-sensitive cells is their inevitable progression to a steroid-insensitive state; current therapies are based on the assumption that hormone insensitivity is associated with loss of receptor. We demonstrate for the first time that breast tumor cells can progress...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90121-8
更新日期:1987-11-20 00:00:00
abstract::Negative selection with FUdR produced revertants from the transformed rat line 14B, which contains one insertion of the SV40 viral genome (Botchan, Topp and Sambrook, 1976). 14B contains nuclear T antigen, grows to a high density, grows in low serum and is anchorage-independent. The revertants fall into three classes ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90134-4
更新日期:1978-01-01 00:00:00
abstract::Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.01.015
更新日期:2010-01-22 00:00:00
abstract::Ventral midline cells at different rostrocaudal levels of the central nervous system exhibit distinct properties but share the ability to pattern the dorsoventral axis of the neural tube. We show here that ventral midline cells acquire distinct identities in response to the different signaling activities of underlying...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80334-7
更新日期:1997-07-25 00:00:00
abstract::The role of electricity in biological systems was first appreciated through electrical stimulation experiments performed by Luigi Galvani in the 18th century. These pioneering experiments demonstrated that the behavior of living tissues is governed by the flow of electrochemical species-an insight that gave rise to th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.06.026
更新日期:2017-06-29 00:00:00
abstract::Two independent approaches to understanding the molecular mechanism of cytokinesis have converged on the gene spaghetti-squash (sqh). A genetic screen for mitotic mutants identified sqh1, a mutation that disrupts cytokinesis, which was then cloned by transposon tagging. Independently, the gene that encodes the regulat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90013-o
更新日期:1991-06-28 00:00:00
abstract::T lymphocyte selection and lineage commitment in the thymus requires multiple signals. Herein, CD4+ T cell generation required engagement of CD83, a surface molecule expressed by thymic epithelial and dendritic cells. CD83-deficient (CD83-/-) mice had a specific block in CD4+ single-positive thymocyte development with...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00673-6
更新日期:2002-03-22 00:00:00
abstract::Replicative intermediates of SV40 were isolated, digested with the restriction endonuclease Bgl I and examined by electron microscopy. Over 98% of the replicative intermediates isolated following infection with wild-type virions at 33 degrees, 37 degrees or 40 degrees C or with tsA209 at 33 degrees C had initiated rep...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90624-8
更新日期:1980-06-01 00:00:00
abstract::Autophagy (literally self-eating) has diverse cytoprotective functions but also is linked to programmed cell death (PCD). In this issue, Qu et al. (2007) analyze the role of autophagy in PCD during development. Unexpectedly they find that autophagy induces apoptotic cells to present a signal to ensure their clearance,...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2007.02.023
更新日期:2007-03-09 00:00:00