Abstract：
:The preprotein translocase of the outer membrane of mitochondria (TOM complex) facilitates the recognition, insertion, and translocation of nuclear-encoded mitochondrial preproteins. We have purified the TOM complex from Neurospora crassa and analyzed its composition and functional properties. The TOM complex contains a cation-selective high-conductance channel. Upon reconstitution into liposomes, it mediates integration of proteins into and translocation across the lipid bilayer. TOM complex particles have a diameter of about 138 A, as revealed by electron microscopy and image analysis; they contain two or three centers of stain-filled openings, which we interpret as pores with an apparent diameter of about 20 A. We conclude that the structure reported here represents the protein-conducting channel of the mitochondrial outer membrane.
journal_name
Celljournal_title
Cellauthors
Künkele KP,Heins S,Dembowski M,Nargang FE,Benz R,Thieffry M,Walz J,Lill R,Nussberger S,Neupert Wdoi
10.1016/s0092-8674(00)81206-4subject
Has Abstractpub_date
1998-06-12 00:00:00pages
1009-19issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81206-4journal_volume
93pub_type
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