The preprotein translocation channel of the outer membrane of mitochondria.


:The preprotein translocase of the outer membrane of mitochondria (TOM complex) facilitates the recognition, insertion, and translocation of nuclear-encoded mitochondrial preproteins. We have purified the TOM complex from Neurospora crassa and analyzed its composition and functional properties. The TOM complex contains a cation-selective high-conductance channel. Upon reconstitution into liposomes, it mediates integration of proteins into and translocation across the lipid bilayer. TOM complex particles have a diameter of about 138 A, as revealed by electron microscopy and image analysis; they contain two or three centers of stain-filled openings, which we interpret as pores with an apparent diameter of about 20 A. We conclude that the structure reported here represents the protein-conducting channel of the mitochondrial outer membrane.






Künkele KP,Heins S,Dembowski M,Nargang FE,Benz R,Thieffry M,Walz J,Lill R,Nussberger S,Neupert W




Has Abstract


1998-06-12 00:00:00
















  • Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

    abstract::Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequen...


    pub_type: 杂志文章


    authors: Volz E,Hill V,McCrone JT,Price A,Jorgensen D,O'Toole Á,Southgate J,Johnson R,Jackson B,Nascimento FF,Rey SM,Nicholls SM,Colquhoun RM,da Silva Filipe A,Shepherd J,Pascall DJ,Shah R,Jesudason N,Li K,Jarrett R,Pacchi

    更新日期:2021-01-07 00:00:00

  • Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.

    abstract::The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast S...


    pub_type: 杂志文章


    authors: Hanein D,Matlack KE,Jungnickel B,Plath K,Kalies KU,Miller KR,Rapoport TA,Akey CW

    更新日期:1996-11-15 00:00:00

  • Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing.

    abstract::Alternative splicing (AS) patterns have diverged rapidly during vertebrate evolution, yet the functions of most species- and lineage-specific splicing events are not known. We observe that mammalian-specific AS events are enriched in transcript sequences encoding intrinsically disordered regions (IDRs) of proteins, in...


    pub_type: 杂志文章


    authors: Gueroussov S,Weatheritt RJ,O'Hanlon D,Lin ZY,Narula A,Gingras AC,Blencowe BJ

    更新日期:2017-07-13 00:00:00

  • Travel Less. Make It Worthwhile.

    abstract::Academic travel has a substantial carbon footprint. The ongoing pandemic has propelled the development and adoption of technologies for online delivery of seminars and remote attendance at scientific conferences. This should not lead to the complete elimination of in-person events, but the scientific community must se...


    pub_type: 杂志文章


    authors: Geitmann A

    更新日期:2020-08-20 00:00:00

  • Human l1 retrotransposition is associated with genetic instability in vivo.

    abstract::Retrotransposons have shaped eukaryotic genomes for millions of years. To analyze the consequences of human L1 retrotransposition, we developed a genetic system to recover many new L1 insertions in somatic cells. Forty-two de novo integrants were recovered that faithfully mimic many aspects of L1s that accumulated sin...


    pub_type: 杂志文章


    authors: Symer DE,Connelly C,Szak ST,Caputo EM,Cost GJ,Parmigiani G,Boeke JD

    更新日期:2002-08-09 00:00:00

  • A novel human p53 isoform is an essential element of the ATR-intra-S phase checkpoint.

    abstract::The archetypal human tumor suppressor p53 is considered to have unique transactivation properties. The assumption is based on the fact that additionally identified human p53 isoforms lack transcriptional activity. However, we provide evidence for the existence of an alternatively spliced p53 isoform (Deltap53) that ex...


    pub_type: 杂志文章


    authors: Rohaly G,Chemnitz J,Dehde S,Nunez AM,Heukeshoven J,Deppert W,Dornreiter I

    更新日期:2005-07-15 00:00:00

  • Malaria virulence genes controlling expression through chromatin modification.

    abstract::Mutually exclusive expression within the var gene family of the malaria parasite Plasmodium falciparum is important for parasite survival and virulence. In this issue of Cell, Duraisingh et al. and Freitas-Junior et al. provide evidence for the role of Sir2-dependent alterations in chromatin structure and changes in s...


    pub_type: 评论,杂志文章


    authors: Deitsch KW

    更新日期:2005-04-08 00:00:00

  • The key to left-right asymmetry.

    abstract::Establishment of left-right asymmetry in vertebrates involves cilia as essential components in the breaking of symmetry, an asymmetric signaling cascade, and a midline barrier that helps to maintain asymmetry. A new study suggests that a reaction-diffusion mechanism also plays a key role. ...


    pub_type: 杂志文章


    authors: Tabin CJ

    更新日期:2006-10-06 00:00:00

  • brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans.

    abstract::The brlA gene of A. nidulans mediates the developmental switch from the indeterminate, apical growth pattern of vegetative cells to the budding growth pattern of conidiophores. brlA encodes a 432 amino acid polypeptide containing two directly repeated motifs resembling the Zn(II) coordination sites first recognized in...


    pub_type: 杂志文章


    authors: Adams TH,Boylan MT,Timberlake WE

    更新日期:1988-07-29 00:00:00

  • Trans-activation by HIV-1 Tat via a heterologous RNA binding protein.

    abstract::The HIV-1 trans-activator Tat increases levels of viral gene expression and replication. The target for Tat is an RNA stem-loop called TAR, located at the 5' end of all viral transcripts. To study the mechanism of action and map functional domains of Tat, we fused Tat to the coat protein of bacteriophage MS2, an RNA b...


    pub_type: 杂志文章


    authors: Selby MJ,Peterlin BM

    更新日期:1990-08-24 00:00:00

  • Disentangling inborn and acquired immunity in human twins.

    abstract::The human geneticist Archibald Garrod noted in 1931 that, "It is, of necessity, no easy matter to distinguish between immunity which is inborn and that which has been acquired" (The Inborn Factors in Disease). In this issue of Cell, Brodin et al. show that the heritability of blood counts rapidly decreases with age fo...


    pub_type: 评论,杂志文章


    authors: Casanova JL,Abel L

    更新日期:2015-01-15 00:00:00

  • Primary structure and transcriptional regulation of GAP-43, a protein associated with nerve growth.

    abstract::Nerve regeneration and developmental outgrowth of axons are both correlated with increased synthesis of an axonal membrane protein designated GAP-43. Phosphorylation of an apparently identical protein, present at lower abundance in adult brains, has been correlated with long-term potentiation, a form of synaptic plast...


    pub_type: 杂志文章


    authors: Basi GS,Jacobson RD,Virág I,Schilling J,Skene JH

    更新日期:1987-06-19 00:00:00

  • M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors.

    abstract::M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...


    pub_type: 杂志文章


    authors: van der Putten H,Quint W,van Raaij J,Maandag ER,Verma IM,Berns A

    更新日期:1981-06-01 00:00:00

  • Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.

    abstract::Peptide growth factors may play a role in patterning of the early embryo, particularly in the induction of mesoderm. We have explored the role of fibroblast growth factor (FGF) in early Xenopus development by expressing a dominant negative mutant form of the FGF receptor. Using a functional assay in frog oocytes, we f...


    pub_type: 杂志文章


    authors: Amaya E,Musci TJ,Kirschner MW

    更新日期:1991-07-26 00:00:00

  • The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner.

    abstract::E2F is a transcription factor believed to play a role in the activation of genes required for cellular proliferation. Its regulation is likely important for maintenance of G0 and for the initiation of cell growth. The retinoblastoma product (RB) forms a complex with E2F in G1 in primary and established human cells. As...


    pub_type: 杂志文章


    authors: Shirodkar S,Ewen M,DeCaprio JA,Morgan J,Livingston DM,Chittenden T

    更新日期:1992-01-10 00:00:00

  • HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells.

    abstract::O(2) is the ultimate electron acceptor for mitochondrial respiration, a process catalyzed by cytochrome c oxidase (COX). In yeast, COX subunit composition is regulated by COX5a and COX5b gene transcription in response to high and low O(2), respectively. Here we demonstrate that in mammalian cells, expression of the CO...


    pub_type: 杂志文章


    authors: Fukuda R,Zhang H,Kim JW,Shimoda L,Dang CV,Semenza GL

    更新日期:2007-04-06 00:00:00

  • Genomic and Transcriptomic Features of Response to Anti-PD-1 Therapy in Metastatic Melanoma.

    abstract::PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...


    pub_type: 评论,杂志文章


    authors: Hugo W,Zaretsky JM,Sun L,Song C,Moreno BH,Hu-Lieskovan S,Berent-Maoz B,Pang J,Chmielowski B,Cherry G,Seja E,Lomeli S,Kong X,Kelley MC,Sosman JA,Johnson DB,Ribas A,Lo RS

    更新日期:2016-03-24 00:00:00

  • Drug-Driven Phenotypic Convergence Supports Rational Treatment Strategies of Chronic Infections.

    abstract::Chronic Pseudomonas aeruginosa infections evade antibiotic therapy and are associated with mortality in cystic fibrosis (CF) patients. We find that in vitro resistance evolution of P. aeruginosa toward clinically relevant antibiotics leads to phenotypic convergence toward distinct states. These states are associated w...


    pub_type: 杂志文章


    authors: Imamovic L,Ellabaan MMH,Dantas Machado AM,Citterio L,Wulff T,Molin S,Krogh Johansen H,Sommer MOA

    更新日期:2018-01-11 00:00:00

  • A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor.

    abstract::Cellular receptors are required for efficient entry of retroviruses into cells. We previously cloned a chicken gene responsible for susceptibility to the retrovirus subgroup A Rous sarcoma virus (RSV(A)). Here we have isolated the quail homolog and generated two alternatively spliced processed genes encoding cellular ...


    pub_type: 杂志文章


    authors: Bates P,Young JA,Varmus HE

    更新日期:1993-09-24 00:00:00

  • Bcl-x(L) retrotranslocates Bax from the mitochondria into the cytosol.

    abstract::The Bcl-2 family member Bax translocates from the cytosol to mitochondria, where it oligomerizes and permeabilizes the mitochondrial outer membrane to promote apoptosis. Bax activity is counteracted by prosurvival Bcl-2 proteins, but how they inhibit Bax remains controversial because they neither colocalize nor form s...


    pub_type: 杂志文章


    authors: Edlich F,Banerjee S,Suzuki M,Cleland MM,Arnoult D,Wang C,Neutzner A,Tjandra N,Youle RJ

    更新日期:2011-04-01 00:00:00

  • Effects of androgens on the complexity of poly(A) RNA from rat prostate.

    abstract::Poly(A)-containing RNA, isolated from rat ventral prostate, has been analyzed for its base sequence complexity. The kinetics of hybridization of total cellular poly(A)-containing RNA with its complementary DNA (cDNA) from normal and castrated animals are markedly different. RNA from normal animals consists of three ab...


    pub_type: 杂志文章


    authors: Parker MG,Mainwaring WI

    更新日期:1977-10-01 00:00:00

  • Crystal structure of the glucocorticoid receptor ligand binding domain reveals a novel mode of receptor dimerization and coactivator recognition.

    abstract::Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...


    pub_type: 杂志文章


    authors: Bledsoe RK,Montana VG,Stanley TB,Delves CJ,Apolito CJ,McKee DD,Consler TG,Parks DJ,Stewart EL,Willson TM,Lambert MH,Moore JT,Pearce KH,Xu HE

    更新日期:2002-07-12 00:00:00

  • A novel glycosphingolipid that may participate in embryo inversion in Volvox carteri.

    abstract::Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...


    pub_type: 杂志文章


    authors: Wenzl S,Sumper M

    更新日期:1986-08-15 00:00:00

  • Downregulation of diacylglycerol kinase delta contributes to hyperglycemia-induced insulin resistance.

    abstract::Type 2 (non-insulin-dependent) diabetes mellitus is a progressive metabolic disorder arising from genetic and environmental factors that impair beta cell function and insulin action in peripheral tissues. We identified reduced diacylglycerol kinase delta (DGKdelta) expression and DGK activity in skeletal muscle from t...


    pub_type: 杂志文章


    authors: Chibalin AV,Leng Y,Vieira E,Krook A,Björnholm M,Long YC,Kotova O,Zhong Z,Sakane F,Steiler T,Nylén C,Wang J,Laakso M,Topham MK,Gilbert M,Wallberg-Henriksson H,Zierath JR

    更新日期:2008-02-08 00:00:00

  • Viral RNAs: lessons from the enemy.

    abstract::Viruses are adept at evolving or co-opting genomic elements that allow them to maximize their replication potential in the infected host. This evolutionary plasticity makes viruses an invaluable system to identify new mechanisms used not only by viruses but also by vertebrate cells to modulate gene expression. Here, I...


    pub_type: 杂志文章


    authors: Cullen BR

    更新日期:2009-02-20 00:00:00

  • Observing a DNA polymerase choose right from wrong.

    abstract::DNA polymerase (pol) β is a model polymerase involved in gap-filling DNA synthesis utilizing two metals to facilitate nucleotidyl transfer. Previous structural studies have trapped catalytic intermediates by utilizing substrate analogs (dideoxy-terminated primer or nonhydrolysable incoming nucleotide). To identify add...


    pub_type: 杂志文章


    authors: Freudenthal BD,Beard WA,Shock DD,Wilson SH

    更新日期:2013-07-03 00:00:00

  • Molecular cloning of cDNA for human von Willebrand factor: authentication by a new method.

    abstract::We have identified a 2.4 kb partial cDNA clone (pDL34) for human von Willebrand factor (vWf) mRNA. pDL34 was selected by screening an endothelial cell cDNA library with a radiolabeled reverse transcript of mRNA obtained by specific immunoisolation of vWf polysomes from endothelial cells. pDL34 selectively hybridized t...


    pub_type: 杂志文章


    authors: Lynch DC,Zimmerman TS,Collins CJ,Brown M,Morin MJ,Ling EH,Livingston DM

    更新日期:1985-05-01 00:00:00

  • Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.

    abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...


    pub_type: 杂志文章


    authors: Orchard RC,Kittisopikul M,Altschuler SJ,Wu LF,Süel GM,Alto NM

    更新日期:2012-02-17 00:00:00

  • 95 kd sperm proteins bind ZP3 and serve as tyrosine kinase substrates in response to zona binding.

    abstract::In the mouse, the zona pellucida (ZP) glycoprotein ZP3 both binds intact sperm and induces acrosomal exocytosis. The subsequent signaling pathway(s) is still uncertain, but Gi-like proteins have been implicated. By analogy with other signal transduction mechanisms, we examined anti-phosphotyrosine antibody reactivity ...


    pub_type: 杂志文章


    authors: Leyton L,Saling P

    更新日期:1989-06-30 00:00:00

  • The sqt-1 gene of C. elegans encodes a collagen critical for organismal morphogenesis.

    abstract::Different mutations in the sqt-1 gene of C. elegans can lengthen, shorten, or helically twist the entire animal. We have cloned the sqt-1 gene and have shown that it encodes a collagen. sqt-1 was localized to a 35 kb region of DNA by physical mapping of chromosomal deficiencies. A transposon (Tc1)-induced mutation of ...


    pub_type: 杂志文章


    authors: Kramer JM,Johnson JJ,Edgar RS,Basch C,Roberts S

    更新日期:1988-11-18 00:00:00