In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.

Abstract:

:A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xenopus oocytes is capable of supporting transcription of the tRNATrp gene contained on plasmid pBR313. The products are precursor tRNAs which contain the intervening RNA sequence. The Xenopus extract accurately splices the precursor transcript to mature-sized tRNATrp.

journal_name

Cell

journal_title

Cell

authors

Ogden RC,Beckman JS,Abelson J,Kang HS,Söll D,Schmidt O

doi

10.1016/0092-8674(79)90166-1

subject

Has Abstract

pub_date

1979-06-01 00:00:00

pages

399-406

issue

2

eissn

0092-8674

issn

1097-4172

pii

0092-8674(79)90166-1

journal_volume

17

pub_type

杂志文章

相关文献

CELL文献大全
  • A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product.

    abstract::We have identified in mice an allele of a new T cell receptor V beta gene, V beta 17a, whose product is bound by the monoclonal antibody KJ23a. Over 90% of T cell hybridomas prepared from V beta 17a+ T cells of SWR mice respond to allogeneic forms of the IE class II MHC protein, indicating that V beta 17a has an appre...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(87)90567-8

    authors: Kappler JW,Wade T,White J,Kushnir E,Blackman M,Bill J,Roehm N,Marrack P

    更新日期:1987-04-24 00:00:00

  • The ClpXP protease unfolds substrates using a constant rate of pulling but different gears.

    abstract::ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2013.09.022

    authors: Sen M,Maillard RA,Nyquist K,Rodriguez-Aliaga P,Pressé S,Martin A,Bustamante C

    更新日期:2013-10-24 00:00:00

  • No-nonsense functions for long noncoding RNAs.

    abstract::The mysterious secrets of long noncoding RNAs, often referred to as the Dark Matter of the genome, are gradually coming to light. Several recent papers dig deep to reveal surprisingly complex and diverse functions of these enigmatic molecules. ...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2011.03.014

    authors: Nagano T,Fraser P

    更新日期:2011-04-15 00:00:00

  • Pattern recognition receptors: doubling up for the innate immune response.

    abstract::Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0092-8674(02)01201-1

    authors: Gordon S

    更新日期:2002-12-27 00:00:00

  • Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis.

    abstract::Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin subst...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2009.01.033

    authors: Lin YY,Lu JY,Zhang J,Walter W,Dang W,Wan J,Tao SC,Qian J,Zhao Y,Boeke JD,Berger SL,Zhu H

    更新日期:2009-03-20 00:00:00

  • Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain.

    abstract::The tumor suppressor p53 exerts antiproliferation effects through its ability to function as a sequence-specific DNA-binding transcription factor. Here, we demonstrate that p53 can be modified by acetylation both in vivo and in vitro. Remarkably, the site of p53 that is acetylated by its coactivator, p300, resides in ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80521-8

    authors: Gu W,Roeder RG

    更新日期:1997-08-22 00:00:00

  • Tumor promoter-induced changes in the permeability of epithelial cell tight junctions.

    abstract::Treatment of the kidney epithelial cell line MDCK with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a large increase in the permeability of tight junctions within 2-4 hr. This change in transepithelial permeability is accompanied by alterations in cell morphology that include the opening of ti...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(81)90274-9

    authors: Ojakian GK

    更新日期:1981-01-01 00:00:00

  • Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II.

    abstract::Using a defined RNA polymerase II (pol II) transcription system, we have investigated the roles of basal factors at discrete stages during the transcription cycle (e.g., initiation, promoter clearance, and transcript elongation). Abortive initiation assays revealed that TATA-binding protein, transcription factors TFII...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(94)90242-9

    authors: Goodrich JA,Tjian R

    更新日期:1994-04-08 00:00:00

  • DNA double-strand breaks come into focus.

    abstract::The Mre11-Rad50-Nbs1 (MRN) complex senses DNA double-strand breaks and recruits different repair pathway and checkpoint proteins to break foci. Two new studies (Williams et al., 2009; Lloyd et al., 2009) identify Nbs1 as a key factor in this process and reveal how an N-terminal protein recruitment module in Nbs1 binds...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2009.09.017

    authors: Hopfner KP

    更新日期:2009-10-02 00:00:00

  • Treatment of obesity with celastrol.

    abstract::Despite all modern advances in medicine, an effective drug treatment of obesity has not been found yet. Discovery of leptin two decades ago created hopes for treatment of obesity. However, development of leptin resistance has been a big obstacle, mitigating a leptin-centric treatment of obesity. Here, by using in sili...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2015.05.011

    authors: Liu J,Lee J,Salazar Hernandez MA,Mazitschek R,Ozcan U

    更新日期:2015-05-21 00:00:00

  • A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.

    abstract::The sequences corresponding to the 18S and 28S rRNAs have been mapped within a cloned 17 kilobase (kb) fragment formed by Eco R1 cleavage of Drosophila melanogaster rDNA. This fragment, Dm103, represents the longer of two major types of repeating units that are present in the rDNA of this fly, and was cloned as a hybr...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(77)90212-4

    authors: Glover DM,Hogness DS

    更新日期:1977-02-01 00:00:00

  • A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus.

    abstract::A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping se...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(85)80025-8

    authors: Boshart M,Weber F,Jahn G,Dorsch-Häsler K,Fleckenstein B,Schaffner W

    更新日期:1985-06-01 00:00:00

  • The matrix (M) protein of vesicular stomatitis virus regulates transcription.

    abstract::Temperature-sensitive mutants of vesicular stomatitis virus (VSV) belonging to complementation group III contain a lesion in the matrix (M) protein. This results in a 2--5 fold increase in transcription at the nonpermissive temperature. Co-infection of cells with one of these mutants and wild-type virus reverses this ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(78)90069-7

    authors: Clinton GM,Little SP,Hagen FS,Huang AS

    更新日期:1978-12-01 00:00:00

  • Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.

    abstract::We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics of 139 COVID-19 patients representing all levels of disease severity, from serial blood draws collected during the first week of infection following diagnosis. We identify a major shift between mild and moderate diseas...

    journal_title:Cell

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1016/j.cell.2020.10.037

    authors: Su Y,Chen D,Yuan D,Lausted C,Choi J,Dai CL,Voillet V,Duvvuri VR,Scherler K,Troisch P,Baloni P,Qin G,Smith B,Kornilov SA,Rostomily C,Xu A,Li J,Dong S,Rothchild A,Zhou J,Murray K,Edmark R,Hong S,Heath JE,Ear

    更新日期:2020-12-10 00:00:00

  • NMR structure of the bacteriophage lambda N peptide/boxB RNA complex: recognition of a GNRA fold by an arginine-rich motif.

    abstract::The structure of the complex formed by the arginine-rich motif of the transcriptional antitermination protein N of phage lambda and boxB RNA was determined by heteronuclear magnetic resonance spectroscopy. A bent alpha helix in N recognizes primarily the shape and negatively charged surface of the boxB hairpin through...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)81579-2

    authors: Legault P,Li J,Mogridge J,Kay LE,Greenblatt J

    更新日期:1998-04-17 00:00:00

  • A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis.

    abstract::Wnt signaling plays a critical role in embryonic development, and genetic aberrations in this network have been broadly implicated in colorectal cancer. We find that the Wnt receptor Frizzled2 (Fzd2) and its ligands Wnt5a/b are elevated in metastatic liver, lung, colon, and breast cancer cell lines and in high-grade t...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2014.10.032

    authors: Gujral TS,Chan M,Peshkin L,Sorger PK,Kirschner MW,MacBeath G

    更新日期:2014-11-06 00:00:00

  • The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless.

    abstract::We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine resi...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(87)90038-9

    authors: Rijsewijk F,Schuermann M,Wagenaar E,Parren P,Weigel D,Nusse R

    更新日期:1987-08-14 00:00:00

  • Overproduction of the Tn3 transposition protein and its role in DNA transposition.

    abstract::Five single base pair mutations that increase expression of the tnpA (transposase) gene of the Tn3 transposon approximately 30-fold, but which still allow the gene to be regulated, have been isolated by using a generally applicable procedure that involves distally linked lac gene fusions. The mutations, which are all ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(82)90352-x

    authors: Casadaban MJ,Chou J,Cohen SN

    更新日期:1982-02-01 00:00:00

  • Membrane hemifusion: crossing a chasm in two leaps.

    abstract::During membrane fusion, the outer leaflets of the two membranes merge first, whereas the distal membrane leaflets remain separate until the opening of a fusion pore. This intermediate stage, called hemifusion, is a critical event shared by exocytosis, protein trafficking, and viral entry. ...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2005.10.015

    authors: Chernomordik LV,Kozlov MM

    更新日期:2005-11-04 00:00:00

  • Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage.

    abstract::Tissue homeostasis requires maintenance of functional integrity under stress. A central source of stress is mechanical force that acts on cells, their nuclei, and chromatin, but how the genome is protected against mechanical stress is unclear. We show that mechanical stretch deforms the nucleus, which cells initially ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2020.03.052

    authors: Nava MM,Miroshnikova YA,Biggs LC,Whitefield DB,Metge F,Boucas J,Vihinen H,Jokitalo E,Li X,García Arcos JM,Hoffmann B,Merkel R,Niessen CM,Dahl KN,Wickström SA

    更新日期:2020-05-14 00:00:00

  • p53 and its 14 kDa C-terminal domain recognize primary DNA damage in the form of insertion/deletion mismatches.

    abstract::Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesion...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(05)80006-6

    authors: Lee S,Elenbaas B,Levine A,Griffith J

    更新日期:1995-06-30 00:00:00

  • Pattern abnormalities induced by ectopic expression of the Drosophila gene hairy are associated with repression of ftz transcription.

    abstract::The pair-rule segmentation gene hairy (h) is expressed and required in alternate metameres in the early Drosophila embryo. We show that h expression outside these domains, driven by an hsp70 promoter, suppresses the expression of fushi tarazu (ftz). The kinetics of action favor h acting directly as a transcriptional r...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(87)90636-2

    authors: Ish-Horowicz D,Pinchin SM

    更新日期:1987-11-06 00:00:00

  • Temporal regulation of salmonella virulence effector function by proteasome-dependent protein degradation.

    abstract::Salmonella enterica invasion of host cells requires the reversible activation of the Rho-family GTPases Cdc42 and Rac1 by the bacterially encoded GEF SopE and the GAP SptP, which exert their function at different times during infection and are delivered into host cells by a type III secretion system. We found that Sop...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(03)00849-3

    authors: Kubori T,Galán JE

    更新日期:2003-10-31 00:00:00

  • Human anticentromere antibodies: distribution, characterization of antigens, and effect on microtubule organization.

    abstract::Properties of human anticentromere autoantibodies were analyzed. In intact cells or isolated cell fractions, these sera stain the centromeres of mitotic chromosomes and discrete speckles (prekinetochores) in nuclei. Staining is also retained in matrix preparations from nuclei or chromosomes. Immunoprecipitation or imm...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(83)90236-2

    authors: Cox JV,Schenk EA,Olmsted JB

    更新日期:1983-11-01 00:00:00

  • Modeling human nutrition using human embryonic stem cells.

    abstract::Nutrition presents unanswered scientific questions of high public health importance. We envision model systems composed of interacting gastrointestinal and metabolic tissues derived from human embryonic stem cells, populated by gut microbiota. The culture will be embedded in 3D scaffolds, creating a controlled experim...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2015.02.039

    authors: Ben-Zvi D,Melton DA

    更新日期:2015-03-26 00:00:00

  • Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes.

    abstract::The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes. HP1, a heterochromatin...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80415-8

    authors: Pak DT,Pflumm M,Chesnokov I,Huang DW,Kellum R,Marr J,Romanowski P,Botchan MR

    更新日期:1997-10-31 00:00:00

  • Forever young.

    abstract::Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2010.01.015

    authors: Guarente L

    更新日期:2010-01-22 00:00:00

  • Sequences of mouse immunoglobulin light chain genes before and after somatic changes.

    abstract::We have determined the nucleotide sequences of the germ line gene as well as a corresponding somatically mutated and rearranged gene coding for a mouse immunoglobulin lambdaI type light chain. These sequencing studies were carried out on three Eco RI-DNA fragments which had been cloned from BALB/c mouse embryos or a l...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(78)90041-7

    authors: Bernard O,Hozumi N,Tonegawa S

    更新日期:1978-12-01 00:00:00

  • DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88.

    abstract::Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 def...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2012.03.036

    authors: Tarallo V,Hirano Y,Gelfand BD,Dridi S,Kerur N,Kim Y,Cho WG,Kaneko H,Fowler BJ,Bogdanovich S,Albuquerque RJ,Hauswirth WW,Chiodo VA,Kugel JF,Goodrich JA,Ponicsan SL,Chaudhuri G,Murphy MP,Dunaief JL,Ambati BK,Ogura Y

    更新日期:2012-05-11 00:00:00

  • Two amino acids within the knuckle of the first zinc finger specify DNA response element activation by the glucocorticoid receptor.

    abstract::The specificity of target gene activation by steroid receptors is encoded within a small, cysteine-rich domain that is believed to form two zinc-coordinated fingers. Here we show that the ability of glucocorticoid and estrogen receptors to discriminate between their closely related response elements resides in the two...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(89)90050-0

    authors: Danielsen M,Hinck L,Ringold GM

    更新日期:1989-06-30 00:00:00