Abstract：
:Properties of human anticentromere autoantibodies were analyzed. In intact cells or isolated cell fractions, these sera stain the centromeres of mitotic chromosomes and discrete speckles (prekinetochores) in nuclei. Staining is also retained in matrix preparations from nuclei or chromosomes. Immunoprecipitation or immunoblotting demonstrates protein antigens of 14, 20, 23, and 34 kd in HeLa nuclei and chromosomes; immunoprecipitates of nuclei also contain a protein of 15.5 kd. Matrix preparations contain only the 20, 23, and 34 kd species. Absorption of the anticentromere serum with any one of the four nuclear antigens immobilized on nitrocellulose is sufficient to eliminate centromere staining. Using a lysed cell model for microtubule nucleation, anticentromere sera are shown to inhibit specifically the organization of microtubules at the kinetochore.
journal_name
Celljournal_title
Cellauthors
Cox JV,Schenk EA,Olmsted JBdoi
10.1016/0092-8674(83)90236-2subject
Has Abstractpub_date
1983-11-01 00:00:00pages
331-9issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(83)90236-2journal_volume
35pub_type
杂志文章相关文献
CELL文献大全abstract：:The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90149-3
更新日期：1995-12-29 00:00:00
abstract：:We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00700-6
更新日期：2002-04-19 00:00:00
abstract：:A temperature-sensitive mutant of BHK, designated ts BN-2, shows a rapid drop in 3H-thymidine incorporation along with accumulation of the cells in the G1 phase of the cycle when asynchronous cultures are shifted from 33.5 degrees C to the nonpermissive temperature of 39.5 degrees C. Synchronized cultures of ts BN-2 c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90017-x
更新日期：1978-10-01 00:00:00
abstract：:Newly synthesized transcripts are usually spliced during transcription or immediately thereafter. So pre-mRNA splicing has been presumed to occur exclusively in the cell nucleus. In this issue of Cell, Denis et al. (2005) now report the presence of functional spliceosomes and signal-dependent pre-mRNA splicing in the ...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2005.07.016
更新日期：2005-08-12 00:00:00
abstract：:The membrane fusion potential of influenza HA, like many viral membrane-fusion glycoproteins, is generated by proteolytic cleavage of a biosynthetic precursor. The three-dimensional structure of ectodomain of the precursor HA0 has been determined and compared with that of cleaved HA. The cleavage site is a prominent s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81771-7
更新日期：1998-10-30 00:00:00
abstract：:The dual host range virus HIX has been previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology has been mapped by electron microscopic analysis of he...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90350-1
更新日期：1978-08-01 00:00:00
abstract：:Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.11.018
更新日期：2009-01-23 00:00:00
abstract：:To investigate the role of the presumed DNA mismatch repair (MMR) gene Msh2 in genome stability and tumorigenesis, we have generated cells and mice that are deficient for the gene. Msh2-deficient cells have lost mismatch binding and have acquired microsatellite instability, a mutator phenotype, and tolerance to methyl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90319-4
更新日期：1995-07-28 00:00:00
abstract：:G1-specific transcriptional activation by Cln3/CDK initiates the budding yeast cell cycle. To identify targets of Cln3/CDK, we analyzed the SBF and MBF transcription factor complexes by multidimensional protein interaction technology (MudPIT). Whi5 was identified as a stably bound component of SBF but not MBF. Inactiv...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.05.025
更新日期：2004-06-25 00:00:00
abstract：:Inflammation is associated with many neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. In this Review, we discuss inducers, sensors, transducers, and effectors of neuroinflammation that contribute to neuronal dysfunction and death. Al...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2010.02.016
更新日期：2010-03-19 00:00:00
abstract：:In this report, we describe the isolation, sequence, and initial characterization of the cDNA for the muscle-specific regulatory factor skeletal myogenin. Transfection of myogenin into the mesenchymal cell line C3H10T1/2 produces cells expressing muscle-specific markers. Myogenin is absent in undifferentiated cells, p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90583-7
更新日期：1989-02-24 00:00:00
abstract：:Recent work has identified a mouse gene (tsg101) whose inactivation in fibroblasts results in cellular transformation and the ability to produce metastatic tumors in nude mice. Here, we report that the human homolog, TSG101, which we isolated and mapped to chromosome 11, bands 15.1-15.2, a region proposed to contain t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81866-8
更新日期：1997-01-10 00:00:00
abstract：:Fibrillar protein aggregates are the major pathological hallmark of several incurable, age-related, neurodegenerative disorders. These aggregates typically contain aggregation-prone pathogenic proteins, such as amyloid-beta in Alzheimer's disease and alpha-synuclein in Parkinson's disease. It is, however, poorly under...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.07.020
更新日期：2010-08-20 00:00:00
abstract：:Loss of cell cycle control and acquisition of chromosomal rearrangements such as gene amplification often occur during tumor progression, suggesting that they may be correlated. We show here that the wild-type p53 allele is lost when fibroblasts from patients with the Li-Fraumeni syndrome (LFS) are passaged in vitro. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90244-7
更新日期：1992-09-18 00:00:00
abstract：:Two-pore domain (K2P) K(+) channels are major regulators of excitability that endow cells with an outwardly rectifying background "leak" conductance. In some K2P channels, strong voltage-dependent activation has been observed, but the mechanism remains unresolved because they lack a canonical voltage-sensing domain. H...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.02.002
更新日期：2016-02-25 00:00:00
abstract：:In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90066-7
更新日期：1979-10-01 00:00:00
abstract：:The advent of H7N9 in early 2013 is of concern for a number of reasons, including its capability to infect humans, the lack of clarity in the etiology of infection, and because the human population does not have pre-existing immunity to the H7 subtype. Earlier sequence analyses of H7N9 hemagglutinin (HA) point to amin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.05.034
更新日期：2013-06-20 00:00:00
abstract：:Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90568-5
更新日期：1988-05-20 00:00:00
abstract：:Targeting of the RAS pathway has long been a critical therapeutic challenge in oncology. Burgess et al. examine how the relative expression of mutant and wild-type KRAS modulates clonal fitness and sensitivity to MEK inhibitors in a model of KrasG12D mutant acute myeloid leukemia and propose its use as a predictive bi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.02.013
更新日期：2017-02-23 00:00:00
abstract：:The difficulty in identifying ligands for nuclear hormone receptors remains an obstacle to understanding their function. For example, in the fruit fly Drosophila melanogaster, only one of its nuclear receptors has a known ligand. In this issue of Cell, report that the fruit fly E75 nuclear receptor contains heme in it...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2005.07.007
更新日期：2005-07-29 00:00:00
abstract：:The murine major histocompatibility complex I region encodes two class II antigens, I-A and I-E. From a mouse spleen DNA cosmid library of the b haplotype, we isolated a clone containing the entire I-A beta gene and a separate exon encoding a beta-chain second domain (A beta 2). The A beta gene, encompassing more than...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90148-4
更新日期：1983-08-01 00:00:00
abstract：:The immortal strand hypothesis posits that the propensity of stem cell compartments to give rise to cancer in later life can be minimized if stem cells, during the process of self-renewal, retain those DNA strands with the fewest mutations acquired during DNA replication. In this Essay, I explore evidence in support o...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.06.019
更新日期：2007-06-29 00:00:00
abstract：:The treatment of advanced prostate cancer has been transformed by novel antiandrogen therapies such as enzalutamide. Here, we identify induction of glucocorticoid receptor (GR) expression as a common feature of drug-resistant tumors in a credentialed preclinical model, a finding also confirmed in patient samples. GR s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.11.012
更新日期：2013-12-05 00:00:00
abstract：:Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90221-5
更新日期：1982-03-01 00:00:00
abstract：:A recent paper in Cell (Yao et al., 2006) and two papers in Developmental Cell (Tenzen et al., 2006; Zhang et al., 2006) identify a new receptor component for Hedgehog, a key morphogen in embryonic development. Many other proteins that bind to Hedgehog in the extracellular matrix or on the cell surface have been ident...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.04.016
更新日期：2006-05-05 00:00:00
abstract：:Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromer...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80575-9
更新日期：1999-03-05 00:00:00
abstract：:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90343-x
更新日期：1984-03-01 00:00:00
abstract：:Information processing relies on precise patterns of synapses between neurons. The cellular recognition mechanisms regulating this specificity are poorly understood. In the medulla of the Drosophila visual system, different neurons form synaptic connections in different layers. Here, we sought to identify candidate ce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.11.021
更新日期：2015-12-17 00:00:00
abstract：:We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA deg...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00065-9
更新日期：2000-09-15 00:00:00
abstract：:Fos protein can trans-activate AP-1-dependent gene expression and trans-repress the c-fos promoter. Although we find that trans-repression is enhanced by coexpression of c-Jun, it does not require any of the AP-1 or ATF sites in the mouse c-fos promoter. A major target for repression is the serum response element (SRE...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90756-3
更新日期：1989-12-22 00:00:00
