The immortal strand hypothesis: segregation and reconstruction.

abstract：:Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesion...

journal_title：Cell

authors： Lee S,Elenbaas B,Levine A,Griffith J

更新日期：1995-06-30 00:00:00

abstract：:Two independent approaches to understanding the molecular mechanism of cytokinesis have converged on the gene spaghetti-squash (sqh). A genetic screen for mitotic mutants identified sqh1, a mutation that disrupts cytokinesis, which was then cloned by transposon tagging. Independently, the gene that encodes the regulat...

journal_title：Cell

authors： Karess RE,Chang XJ,Edwards KA,Kulkarni S,Aguilera I,Kiehart DP

更新日期：1991-06-28 00:00:00

abstract：:We constructed rabbit beta-globin genes with deletions in the large intron, extending from the midpoint toward the 5' or 3' splice sites. Analysis of transcripts in transformed HeLa cells showed that six 5' proximal intron nucleotides allowed normal splicing. Correct splicing at the 3' splice site required 12 or more ...

journal_title：Cell

authors： Wieringa B,Hofer E,Weissmann C

更新日期：1984-07-01 00:00:00

abstract：:The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is dete...

journal_title：Cell

authors： Stillman BW,Lewis JB,Chow LT,Mathews MB,Smart JE

更新日期：1981-02-01 00:00:00

abstract：:A major challenge to understanding how cells work together in the central nervous system (CNS) is the heterogeneous cellular composition of the brain. In this issue, Heiman et al. (2008) and Doyle et al. (2008) introduce a new strategy (TRAP) that enables the profiling of translated mRNAs in specific CNS cell populati...

journal_title：Cell

authors： Emery B,Barres BA

更新日期：2008-11-14 00:00:00

abstract：:The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct...

journal_title：Cell

authors： Abovich N,Rosbash M

更新日期：1997-05-02 00:00:00

abstract：:Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...

journal_title：Cell

authors： Leslie NR,den Hertog J

更新日期：2014-04-24 00:00:00

abstract：:During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...

journal_title：Cell

authors： Zhu Y,Crowley SC,Latimer AJ,Lewis GM,Nash R,Kucenas S

更新日期：2019-09-19 00:00:00

abstract：:The yeast plasmid 2 micron circle actively maintains high but stable copy levels in the cell, even though the plasmid confers no selective advantage to its host. To address the mechanism by which stable copy control is achieved, we have examined the level of expression of the genes resident on the yeast plasmid 2 micr...

journal_title：Cell

authors： Som T,Armstrong KA,Volkert FC,Broach JR

更新日期：1988-01-15 00:00:00

abstract：:Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12. Four structurally different recombinant plasmid DNAs from transformed clones were charac...

journal_title：Cell

authors： Brown WM,Watson RM,Vinograd J,Tait KM,Boyer HW,Goodman HM

更新日期：1976-04-01 00:00:00

abstract：:Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...

journal_title：Cell

authors： Pollard TD,Borisy GG

更新日期：2003-02-21 00:00:00

abstract：:Signaling by the epidermal growth factor receptor requires an allosteric interaction between the kinase domains of two receptors, whereby one activates the other. We show that the intracellular juxtamembrane segment of the receptor, known to potentiate kinase activity, is able to dimerize the kinase domains. The C-ter...

journal_title：Cell

authors： Jura N,Endres NF,Engel K,Deindl S,Das R,Lamers MH,Wemmer DE,Zhang X,Kuriyan J

更新日期：2009-06-26 00:00:00

abstract：:Most bacteria that colonize eukaryotes must bind directly to host cells to establish a replicative niche. In enteric bacteria, adhesion to host cells is often promoted by a lectin found on surface-localized pili. Some pili promote efficient adhesion only when they are subjected to shear stress, as found during the flo...

journal_title：Cell

authors： Isberg RR,Barnes P

更新日期：2002-07-12 00:00:00

abstract：:Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusi...

journal_title：Cell

authors： Corral J,Lavenir I,Impey H,Warren AJ,Forster A,Larson TA,Bell S,McKenzie AN,King G,Rabbitts TH

更新日期：1996-06-14 00:00:00

abstract：:The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, transcription. Here we describe the isolation and characterization of the first gene en...

journal_title：Cell

authors： Hoey T,Weinzierl RO,Gill G,Chen JL,Dynlacht BD,Tjian R

更新日期：1993-01-29 00:00:00

abstract：:Endophytic fungi are found within the roots of healthy plants, but their function is poorly understood. In this issue, Hiruma et al. demonstrate that, under phosphate-limiting conditions, the endophytic fungus, Colletotrichum tofieldiae, provides growth-promoting and fitness benefits to Arabidopsis, but the plant must...

journal_title：Cell

authors： Liberman LM,Benfey PN

更新日期：2016-04-07 00:00:00

abstract：:We have sequenced HFL1 from D. melanogaster, the only cloned hobo element shown to have transposase activity. The 2959 bp HFL1 sequence predicts a 2.0 kb open reading frame (ORF1) with substantial amino acid similarity to the transposases of Activator (Ac) from maize (Zea mays) and Tam3 from snapdragon (Antirrhinum ma...

journal_title：Cell

authors： Calvi BR,Hong TJ,Findley SD,Gelbart WM

更新日期：1991-08-09 00:00:00

abstract：:The hemagglutinin of influenza virus is synthesized as a monomeric subunit that is cotranslationally translocated across the membrane of the rough endoplasmic reticulum. We show that folding and assembly of hemagglutinin monomers into trimeric structures takes approximately 7-10 min and is completed before the protein...

journal_title：Cell

authors： Gething MJ,McCammon K,Sambrook J

更新日期：1986-09-12 00:00:00

abstract：:The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and somatic cell reprogramming. Here, we review the highly connected protein and transcriptional networks that maintain pluripotency and how they are intertwined with f...

journal_title：Cell

authors： Orkin SH,Hochedlinger K

更新日期：2011-06-10 00:00:00

abstract：:In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...

journal_title：Cell

authors： Guo Y,Li D,Zhang S,Yang Y,Liu JJ,Wang X,Liu C,Milkie DE,Moore RP,Tulu US,Kiehart DP,Hu J,Lippincott-Schwartz J,Betzig E,Li D

更新日期：2018-11-15 00:00:00

abstract：:The mechanism by which cells decide to skip mitosis to become polyploid is largely undefined. Here we used a high-content image-based screen to identify small-molecule probes that induce polyploidization of megakaryocytic leukemia cells and serve as perturbagens to help understand this process. Our study implicates fi...

journal_title：Cell

authors： Wen Q,Goldenson B,Silver SJ,Schenone M,Dancik V,Huang Z,Wang LZ,Lewis TA,An WF,Li X,Bray MA,Thiollier C,Diebold L,Gilles L,Vokes MS,Moore CB,Bliss-Moreau M,Verplank L,Tolliday NJ,Mishra R,Vemula S,Shi J,Wei L

更新日期：2012-08-03 00:00:00

abstract：:The gut epithelium self-renews every several days, providing an important innate defense system that limits bacterial colonization. Nevertheless, many bacterial pathogens, including Shigella, efficiently colonize the intestinal epithelium. Here, we show that the Shigella effector IpaB, when delivered into epithelial c...

journal_title：Cell

authors： Iwai H,Kim M,Yoshikawa Y,Ashida H,Ogawa M,Fujita Y,Muller D,Kirikae T,Jackson PK,Kotani S,Sasakawa C

更新日期：2007-08-24 00:00:00

abstract：:We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly sp...

journal_title：Cell

authors： Bryan PN,Hofstetter H,Birnstiel ML

更新日期：1981-12-01 00:00:00

abstract：:Secretory proteins are segregated from cytosolic proteins by their translocation into the endoplasmic reticulum (ER). A modified secretory protein trapped during translocation across the ER membrane can be crosslinked to two previously identified proteins, Sec61p and BiP (Kar2p). The dependence of this cross-linking u...

journal_title：Cell

authors： Sanders SL,Whitfield KM,Vogel JP,Rose MD,Schekman RW

更新日期：1992-04-17 00:00:00

abstract：:We describe a monoclonal antibody (anti-FC-1) that inhibits the attachment of chicken embryo fibroblasts to artificial substrates. In the immunofluorescence, anti-FC-1 also stained the focal contacts of the fibroblasts, organelles suggested to be involved in cell-substrate adhesion. This antibody was absorbed on the s...

journal_title：Cell

authors： Oesch B,Birchmeier W

更新日期：1982-12-01 00:00:00

abstract：:Seeing begins in the photoreceptors, where light is absorbed and signaled to the nervous system. Throughout the animal kingdom, photoreceptors are diverse in design and purpose. Nonetheless, phototransduction-the mechanism by which absorbed photons are converted into an electrical response-is highly conserved and base...

journal_title：Cell

authors： Yau KW,Hardie RC

更新日期：2009-10-16 00:00:00

abstract：:Treating B16 mouse melanoma cells with monoclonal antibody NORM-2 reduces cell growth in tissue culture, agar, and syngeneic mice. We show that the NORM-2 antibody recognizes an integral 83 kd glycoprotein that is mobile in the plane of the plasma membrane of B16 melanoma cells. Expression of the glycoprotein is reduc...

journal_title：Cell

authors： Wieland I,Müller G,Braun S,Birchmeier W

更新日期：1986-12-05 00:00:00

abstract：:Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...

journal_title：Cell

authors： Selzer G,Som T,Itoh T,Tomizawa J

更新日期：1983-01-01 00:00:00

abstract：:MicroRNAs are small endogenous noncoding RNAs involved in posttranscriptional gene regulation. During microRNA biogenesis, Drosha and Dicer process the primary transcript (pri-miRNA) through a precursor hairpin (pre-miRNA) to the mature miRNA. The miRNA is incorporated into the RNA-Induced Silencing Complex (RISC) wit...

journal_title：Cell

authors： Diederichs S,Haber DA

更新日期：2007-12-14 00:00:00

abstract：:Treatment of the kidney epithelial cell line MDCK with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a large increase in the permeability of tight junctions within 2-4 hr. This change in transepithelial permeability is accompanied by alterations in cell morphology that include the opening of ti...

journal_title：Cell

authors： Ojakian GK

更新日期：1981-01-01 00:00:00