Abstract：
:Signaling by the epidermal growth factor receptor requires an allosteric interaction between the kinase domains of two receptors, whereby one activates the other. We show that the intracellular juxtamembrane segment of the receptor, known to potentiate kinase activity, is able to dimerize the kinase domains. The C-terminal half of the juxtamembrane segment latches the activated kinase domain to the activator, and the N-terminal half of this segment further potentiates dimerization, most likely by forming an antiparallel helical dimer that engages the transmembrane helices of the activated receptor. Our data are consistent with a mechanism in which the extracellular domains block the intrinsic ability of the transmembrane and cytoplasmic domains to dimerize and activate, with ligand binding releasing this block. The formation of the activating juxtamembrane latch is prevented by the C-terminal tails in a structure of an inactive kinase domain dimer, suggesting how alternative dimers can prevent ligand-independent activation.
journal_name
Celljournal_title
Cellauthors
Jura N,Endres NF,Engel K,Deindl S,Das R,Lamers MH,Wemmer DE,Zhang X,Kuriyan Jdoi
10.1016/j.cell.2009.04.025subject
Has Abstractpub_date
2009-06-26 00:00:00pages
1293-307issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(09)00450-4journal_volume
137pub_type
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