Abstract：
:We have isolated three alleles of a novel Drosophila clock gene, double-time (dbt). Short- (dbtS) and long-period (dbtL) mutants alter both behavioral rhythmicity and molecular oscillations from previously identified clock genes, period and timeless. A third allele, dbtP, causes pupal lethality and eliminates circadian cycling of per and tim gene products in larvae. In dbtP mutants, PER proteins constitutively accumulate, remain hypophosphorylated, and no longer depend on TIM proteins for their accumulation. We propose that the normal function of DOUBLETIME protein is to reduce the stability and thus the level of accumulation of monomeric PER proteins. This would promote a delay between per/tim transcription and PER/TIM complex function, which is essential for molecular rhythmicity.
journal_name
Celljournal_title
Cellauthors
Price JL,Blau J,Rothenfluh A,Abodeely M,Kloss B,Young MWdoi
10.1016/s0092-8674(00)81224-6subject
Has Abstractpub_date
1998-07-10 00:00:00pages
83-95issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81224-6journal_volume
94pub_type
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