Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.


:ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial cells (rAC-VECs) without transitioning through a pluripotent state. Transient ETV2 expression in ACs generates immature rAC-VECs, whereas coexpression with FLI1/ERG1 endows rAC-VECs with a vascular repertoire and morphology matching mature endothelial cells (ECs). Brief TGFβ-inhibition functionalizes VEGFR2 signaling, augmenting specification of ACs into rAC-VECs. Genome-wide transcriptional analyses showed that rAC-VECs are similar to adult ECs in which vascular-specific genes are expressed and nonvascular genes are silenced. Functionally, rAC-VECs form stable vasculature in Matrigel plugs and regenerating livers. Therefore, short-term ETV2 expression and TGFβ inhibition with constitutive ERG1/FLI1 coexpression reprogram mature ACs into durable rAC-VECs with clinical-scale expansion potential. Banking of HLA-typed rAC-VECs establishes a vascular inventory for treatment of diverse disorders.






Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S




Has Abstract


2012-10-26 00:00:00
















  • Stringent regulation of stably integrated chloramphenicol acetyl transferase genes by E. coli lac repressor in monkey cells.

    abstract::Monkey cell lines that constitutively synthesize 38.6 kd lac repressor protein and bear stably integrated chloramphenicol acetyl transferase (CAT) genes linked to a lac operator-containing SV40 early promoter-enhancer were generated. When grown in medium containing isopropyl beta-D-thiogalactoside (IPTG), these cells ...


    pub_type: 杂志文章


    authors: Figge J,Wright C,Collins CJ,Roberts TM,Livingston DM

    更新日期:1988-03-11 00:00:00

  • Epigenetics of reprogramming to induced pluripotency.

    abstract::Reprogramming to induced pluripotent stem cells (iPSCs) proceeds in a stepwise manner with reprogramming factor binding, transcription, and chromatin states changing during transitions. Evidence is emerging that epigenetic priming events early in the process may be critical for pluripotency induction later. Chromatin ...


    pub_type: 杂志文章,评审


    authors: Papp B,Plath K

    更新日期:2013-03-14 00:00:00

  • A cell that dies during wild-type C. elegans development can function as a neuron in a ced-3 mutant.

    abstract::Mutations in the C. elegans gene ced-3 prevent almost all programmed cell deaths, so that in a ced-3 mutant there are many extra cells. We show that the pharyngeal neuron M4 is essential for feeding in wild-type worms, but in a ced-3 mutant, one of the extra cells, probably MSpaaaaap (the sister of M4), can sometimes ...


    pub_type: 杂志文章


    authors: Avery L,Horvitz HR

    更新日期:1987-12-24 00:00:00

  • An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene.

    abstract::Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected ...


    pub_type: 杂志文章


    authors: Choi KH,Chen CJ,Kriegler M,Roninson IB

    更新日期:1988-05-20 00:00:00

  • Comparative analysis of the structural organization of two closely related vitellogenin genes in X. laevis.

    abstract::The structural organization of the two closely related vitellogenin genes A1 and A2 has been determined and compared by electron microscopy. In both genes the mRNA-coding sequence of 6 kb is interrupted 33 times, leading to a total gene length of 21 kb for gene A1 and 16 kb for gene A2. Thus both genes have a mean exo...


    pub_type: 杂志文章


    authors: Wahli W,Dawid IB,Wyler T,Weber R,Ryffel GU

    更新日期:1980-05-01 00:00:00

  • Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

    abstract::Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a hig...


    pub_type: 杂志文章


    authors: Cheung P,Vallania F,Warsinske HC,Donato M,Schaffert S,Chang SE,Dvorak M,Dekker CL,Davis MM,Utz PJ,Khatri P,Kuo AJ

    更新日期:2018-05-31 00:00:00

  • 3D Genome of macaque fetal brain reveals evolutionary innovations during primate corticogenesis.

    abstract::Elucidating the regulatory mechanisms of human brain evolution is essential to understanding human cognition and mental disorders. We generated multi-omics profiles and constructed a high-resolution map of 3D genome architecture of rhesus macaque during corticogenesis. By comparing the 3D genomes of human, macaque, an...


    pub_type: 杂志文章


    authors: Luo X,Liu Y,Dang D,Hu T,Hou Y,Meng X,Zhang F,Li T,Wang C,Li M,Wu H,Shen Q,Hu Y,Zeng X,He X,Yan L,Zhang S,Li C,Su B

    更新日期:2021-01-22 00:00:00

  • G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase.

    abstract::The S. cerevisiae CLN genes encode cyclin homologs essential for progression from G1 to S phase. The CLN2 gene encodes a 62 kd polypeptide that accumulates periodically, peaking during G1 and decreasing rapidly thereafter, and is rapidly lost following exposure of cells to mating pheromone. Cln2 abundance can be expla...


    pub_type: 杂志文章


    authors: Wittenberg C,Sugimoto K,Reed SI

    更新日期:1990-07-27 00:00:00

  • Organization and structure of an E. coli tRNA operon containing seven tRNA genes.

    abstract::The structure and organization on the Escherichia coli chromosome of the gene cluster coding for two methionine tRNAs (tRNAmMet), four glutamine tRNAs (two tRNA1Gln and two tRNA2Gln), and a previously unidentified tRNA (called tRNAx) have been studied by restriction enzyme analysis and DNA sequencing, utilizing a spec...


    pub_type: 杂志文章


    authors: Nakajima N,Ozeki H,Shimura Y

    更新日期:1981-01-01 00:00:00

  • Bone and Muscle Endocrine Functions: Unexpected Paradigms of Inter-organ Communication.

    abstract::Most physiological functions originate with the communication between organs. Mouse genetics has revived this holistic view of physiology through the identification of inter-organ communications that are unanticipated, functionally important, and would have been difficult to uncover otherwise. This Review highlights t...


    pub_type: 杂志文章,评审


    authors: Karsenty G,Olson EN

    更新日期:2016-03-10 00:00:00

  • Cellular motility driven by assembly and disassembly of actin filaments.

    abstract::Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...


    pub_type: 杂志文章,评审


    authors: Pollard TD,Borisy GG

    更新日期:2003-02-21 00:00:00

  • Structure of the 3' hairpin termini of four rodent parvovirus genomes: nucleotide sequence homology at origins of DNA replication.

    abstract::The nucleotide sequences of the 3' termini of the DNA from four autonomous rodent parvoviruses have been determined. The terminus of each genome exists as a Y-shaped hairpin structure involving 115 or 116 nucleotides. The sequence of this region of DNA is highly conserved and shows no evidence of internal sequence het...


    pub_type: 杂志文章


    authors: Astell CR,Smith M,Chow MB,Ward DC

    更新日期:1979-07-01 00:00:00

  • Deconvolving the recognition of DNA shape from sequence.

    abstract::Protein-DNA binding is mediated by the recognition of the chemical signatures of the DNA bases and the 3D shape of the DNA molecule. Because DNA shape is a consequence of sequence, it is difficult to dissociate these modes of recognition. Here, we tease them apart in the context of Hox-DNA binding by mutating residues...


    pub_type: 杂志文章


    authors: Abe N,Dror I,Yang L,Slattery M,Zhou T,Bussemaker HJ,Rohs R,Mann RS

    更新日期:2015-04-09 00:00:00

  • Alternative splicing accounts for the four forms of myelin basic protein.

    abstract::We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...


    pub_type: 杂志文章


    authors: de Ferra F,Engh H,Hudson L,Kamholz J,Puckett C,Molineaux S,Lazzarini RA

    更新日期:1985-12-01 00:00:00

  • Membrane hemifusion: crossing a chasm in two leaps.

    abstract::During membrane fusion, the outer leaflets of the two membranes merge first, whereas the distal membrane leaflets remain separate until the opening of a fusion pore. This intermediate stage, called hemifusion, is a critical event shared by exocytosis, protein trafficking, and viral entry. ...


    pub_type: 杂志文章,评审


    authors: Chernomordik LV,Kozlov MM

    更新日期:2005-11-04 00:00:00

  • Displacement of sequence-specific transcription factors from mitotic chromatin.

    abstract::The general inhibition in transcriptional activity during mitosis abolishes the stress-inducible expression of the human hsp70 gene. Among the four transcription factors that bind to the human hsp70 promoter, the DNA-binding activities of three (C/EBP, GBP, and HSF1) were normal, while Sp1 showed reduced binding activ...


    pub_type: 杂志文章


    authors: Martínez-Balbás MA,Dey A,Rabindran SK,Ozato K,Wu C

    更新日期:1995-10-06 00:00:00

  • An Akt/beta-arrestin 2/PP2A signaling complex mediates dopaminergic neurotransmission and behavior.

    abstract::Dopamine plays an important role in the etiology of schizophrenia, and D2 class dopamine receptors are the best-established target of antipsychotic drugs. Here we show that D2 class-receptor-mediated Akt regulation involves the formation of signaling complexes containing beta-arrestin 2, PP2A, and Akt. beta-arrestin 2...


    pub_type: 杂志文章


    authors: Beaulieu JM,Sotnikova TD,Marion S,Lefkowitz RJ,Gainetdinov RR,Caron MG

    更新日期:2005-07-29 00:00:00

  • Solving mysteries of DNA replication and frog cloning.

    abstract::Compared to sperm nuclei, nuclei from adult somatic cells replicate inefficiently in frog egg extract. In this issue of Cell, Lemaitre et al. (2005) show that pre-exposure of erythrocyte nuclei to a mitotic extract removes this difference, reorganizes the chromatin into shorter loops, and allows replication at much sh...


    pub_type: 评论,杂志文章


    authors: Laskey R

    更新日期:2005-12-02 00:00:00

  • Function of E. coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing.

    abstract::The sigma factor sigma 70 of E. coli RNA polymerase acts not only in initiation, but also at an early stage of elongation to induce a transcription pause, and simultaneously to allow the phage lambda gene Q transcription antiterminator to act. We identify the signal in DNA that induces early pausing to be a version of...


    pub_type: 杂志文章


    authors: Ring BZ,Yarnell WS,Roberts JW

    更新日期:1996-08-09 00:00:00

  • Histone acetyltransferase complexes stabilize swi/snf binding to promoter nucleosomes.

    abstract::To investigate the function of SWI/SNF in site-specific chromatin remodeling at promoters, we have used a purified system to analyze its distribution, function, and retention following recruitment by a sequence-specific transcription activator. Activator recruitment of SWI/SNF bound the complex to promoter proximal nu...


    pub_type: 杂志文章


    authors: Hassan AH,Neely KE,Workman JL

    更新日期:2001-03-23 00:00:00

  • Binding of a high affinity phosphotyrosyl peptide to the Src SH2 domain: crystal structures of the complexed and peptide-free forms.

    abstract::The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and...


    pub_type: 杂志文章


    authors: Waksman G,Shoelson SE,Pant N,Cowburn D,Kuriyan J

    更新日期:1993-03-12 00:00:00

  • Lipid-Sorting Specificity Encoded in K-Ras Membrane Anchor Regulates Signal Output.

    abstract::K-Ras is targeted to the plasma membrane by a C-terminal membrane anchor that comprises a farnesyl-cysteine-methyl-ester and a polybasic domain. We used quantitative spatial imaging and atomistic molecular dynamics simulations to examine molecular details of K-Ras plasma membrane binding. We found that the K-Ras ancho...


    pub_type: 杂志文章


    authors: Zhou Y,Prakash P,Liang H,Cho KJ,Gorfe AA,Hancock JF

    更新日期:2017-01-12 00:00:00

  • A dynamically localized protease complex and a polar specificity factor control a cell cycle master regulator.

    abstract::Regulated proteolysis is essential for cell cycle progression in both prokaryotes and eukaryotes. We show here that the ClpXP protease, responsible for the degradation of multiple bacterial proteins, is dynamically localized to specific cellular positions in Caulobacter where it degrades colocalized proteins. The CtrA...


    pub_type: 杂志文章


    authors: McGrath PT,Iniesta AA,Ryan KR,Shapiro L,McAdams HH

    更新日期:2006-02-10 00:00:00

  • HIV-1 at 25.

    abstract::In 1981, the acquired immunodeficiency syndrome (AIDS) appeared insidiously and mystified doctors and scientists alike. No one could have predicted then that it would become, arguably, the worst plague in human history. Today, 33 million persons are living with infection by the human immunodeficiency virus type 1 (HIV...


    pub_type: 杂志文章


    authors: Ho DD,Bieniasz PD

    更新日期:2008-05-16 00:00:00

  • Location of sequences on the adenovirus genome coding for the 5.5S RNA.

    abstract::The origin of a low molecular weight virus-associated RNA (VA-RNA) was mapped by hybridization of VA-RNA to specific fragments of adenovirus type 2 DNA, obtained after cleavage with three different restriction endonucleases. VA-RNA was found to hybridize exculsively to the l-strand [strand with low buoyant density in ...


    pub_type: 杂志文章


    authors: Pettersson U,Philipson L

    更新日期:1975-09-01 00:00:00

  • Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.

    abstract::We present evidence that the genes encoding U3 snRNA in plants are transcribed by RNA polymerase III (pol III) and not by RNA polymerase II (pol II) as in vertebrates or lower eukaryotes. The U3 gene is the only known example of a gene transcribed by different polymerases in different organisms. It is possible to conv...


    pub_type: 杂志文章


    authors: Kiss T,Marshallsay C,Filipowicz W

    更新日期:1991-05-03 00:00:00

  • Breast cancer stem cells: eradication by differentiation therapy?

    abstract::During metastasis, migrating breast cancer stem cells undergo a loss of polarity leading to an epithelial-to-mesenchymal transition (EMT). Gupta et al. (2009) use this attribute of cancer stem cells to develop a high-throughput screen, which successfully identifies small molecules that specifically inhibit cancer stem...


    pub_type: 评论,杂志文章


    authors: Beug H

    更新日期:2009-08-21 00:00:00

  • Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.

    abstract::A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...


    pub_type: 杂志文章


    authors: Zuber P,Losick R

    更新日期:1983-11-01 00:00:00

  • Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

    abstract::Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...


    pub_type: 杂志文章


    authors: Brady J,Radonovich M,Vodkin M,Natarajan V,Thoren M,Das G,Janik J,Salzman NP

    更新日期:1982-12-01 00:00:00

  • No-nonsense functions for long noncoding RNAs.

    abstract::The mysterious secrets of long noncoding RNAs, often referred to as the Dark Matter of the genome, are gradually coming to light. Several recent papers dig deep to reveal surprisingly complex and diverse functions of these enigmatic molecules. ...


    pub_type: 杂志文章,评审


    authors: Nagano T,Fraser P

    更新日期:2011-04-15 00:00:00