Abstract:
:Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites is an important determinant in the selection of splice-sites. Deletion, substitution, or even subtle changes of exon sequences can alter the pattern of splice-site selection, and in many cases the splice site adjacent to the altered exon is not used. The relative use of the duplicated splice sites can also be altered by diluting the splicing extract, suggesting that factors involved in splice-site selection are limiting.
journal_name
Celljournal_title
Cellauthors
Reed R,Maniatis Tdoi
10.1016/0092-8674(86)90343-0subject
Has Abstractpub_date
1986-08-29 00:00:00pages
681-90issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(86)90343-0journal_volume
46pub_type
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