Abstract:
:Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational symmetry are present in each of the sites. Superimposed on each operator is an E. coli RNA polymerase recognition site (promoter). Similarities in the sequences of the two lambda promoters, a lac promoter, and an E. coli RNA polymerase recognition site in SV40 DNA are noted.
journal_name
Celljournal_title
Cellauthors
Maniatis T,Ptashne M,Backman K,Kield D,Flashman S,Jeffrey A,Maurer Rdoi
10.1016/0092-8674(75)90018-5subject
Has Abstractpub_date
1975-06-01 00:00:00pages
109-13issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(75)90018-5journal_volume
5pub_type
杂志文章相关文献
CELL文献大全abstract::Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to development...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00003-5
更新日期:2003-01-24 00:00:00
abstract::We studied the chromatin structure and methylation of ribosomal RNA genes (rDNA) in hybrids between Xenopus laevis and Xenopus borealis. S1-nuclease protection experiments showed that 97%-98% of the rRNA precursor in hybrid tadpoles was of the X. laevis type. Preferential expression of the laevis rDNA was correlated w...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90105-2
更新日期:1982-05-01 00:00:00
abstract::The rapid cell proliferation characteristic of early animal embryos is accomplished with an abbreviated cell cycle and no DNA replication checkpoint. Blythe and Wieschaus provide evidence that nascent zygotic transcription precedes—and may trigger—this checkpoint at the midblastula transition. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.02.051
更新日期:2015-03-12 00:00:00
abstract::We have previously cloned lethal(2)giant larvae, a tumor-suppressor gene of Drosophila that normally controls cell proliferation and/or differentiation in the optic centers of the brain and the imaginal discs. Here we describe the structure of the l(2)gl genes as determined by sequencing genomic and cDNA clones. The s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90217-0
更新日期:1987-07-17 00:00:00
abstract::p11, through unknown mechanisms, is required for behavioral and cellular responses to selective serotonin reuptake inhibitors (SSRIs). We show that SMARCA3, a chromatin-remodeling factor, is a target for the p11/annexin A2 heterotetrameric complex. Determination of the crystal structure indicates that SMARCA3 peptide ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.01.014
更新日期:2013-02-14 00:00:00
abstract::The JAK/STAT signaling pathway, renowned for its effects on cell proliferation and survival, is constitutively active in various human cancers, including ovarian. We have found that JAK and STAT are required to convert the border cells in the Drosophila ovary from stationary, epithelial cells to migratory, invasive ce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00607-9
更新日期:2001-12-28 00:00:00
abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.014
更新日期:2015-06-04 00:00:00
abstract::Invasive lobular carcinoma (ILC) is the second most prevalent histologic subtype of invasive breast cancer. Here, we comprehensively profiled 817 breast tumors, including 127 ILC, 490 ductal (IDC), and 88 mixed IDC/ILC. Besides E-cadherin loss, the best known ILC genetic hallmark, we identified mutations targeting PTE...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.09.033
更新日期:2015-10-08 00:00:00
abstract::Reactive oxygen species (ROS) and mitochondrial defects in neurons are implicated in neurodegenerative disease. Here, we find that a key consequence of ROS and neuronal mitochondrial dysfunction is the accumulation of lipid droplets (LD) in glia. In Drosophila, ROS triggers c-Jun-N-terminal Kinase (JNK) and Sterol Reg...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.12.019
更新日期:2015-01-15 00:00:00
abstract::Retrograde transport, in which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions. Mechanistic studies that explore the molecular machinery involved in this retrograde trafficking route are sheddin...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2008.12.009
更新日期:2008-12-26 00:00:00
abstract::Neurotrophic factors are essential for neuronal survival and function. Recent data have demonstrated that the product of the tyrosine kinase trk proto-oncogene binds NGF and is a component of the high affinity NGF receptor. Analysis of the trkB gene product, gp145trkB, in NIH 3T3 cells indicates that this tyrosine kin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90396-g
更新日期:1991-05-31 00:00:00
abstract::Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles. It has been postulated to dock vesicles to their release sites, to act as the Ca2+ sensor for the release process, and to be a fusion protein during exocytosis. To clarify the function of this protein, we have undertaken a genetic analy...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90356-u
更新日期:1993-07-02 00:00:00
abstract::Gold conjugates have been used to quantitate human transferrin receptors (hTfnRs) on transfected chick embryo fibroblasts. No relationship could be found between the number of hTfnRs and the number of clathrin-coated pits. However, hTfnRs are also associated with flat clathrin lattices that lie outside invaginated pit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90094-f
更新日期:1991-05-17 00:00:00
abstract::Loss of cell cycle control and acquisition of chromosomal rearrangements such as gene amplification often occur during tumor progression, suggesting that they may be correlated. We show here that the wild-type p53 allele is lost when fibroblasts from patients with the Li-Fraumeni syndrome (LFS) are passaged in vitro. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90244-7
更新日期:1992-09-18 00:00:00
abstract::We propose a mechanism for the priming of influenza viral RNA transcription by capped RNAs in which specific 5'-terminal fragments are cleaved from the capped RNAs by a virion-associated endonuclease. These fragments would serve as the actual primers for the initiation of transcription by the initial incorporation by ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90449-9
更新日期:1981-03-01 00:00:00
abstract::The 5' terminal sequences of several adenovirus 2 (Ad2) mRNAs, isolated late in infection, are complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed. This has been observed by forming RNA displacement loops (R loops) between Ad2 D...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90180-5
更新日期:1977-09-01 00:00:00
abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90221-5
更新日期:1982-03-01 00:00:00
abstract::Cardiomyocytes, the cells of the heart muscle, lose nearly all of their proliferative capacity after birth, limiting the heart's ability to regenerate. Naqvi et al. now identify a transient burst of cardiomyocyte proliferation during preadolescence, driven by a thyroid hormone surge, with therapeutic implications for ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.04.025
更新日期:2014-05-08 00:00:00
abstract::In ciliates, small RNAs have been shown to target foreign sequences for silencing via elimination from the somatic genome. Fang et al. now reveal a set of Piwi-interacting RNAs (piRNAs) in Oxytricha trifallax that likewise enable genomic self versus nonself discrimination, this time by specifying self sequences for ge...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.11.032
更新日期:2012-12-07 00:00:00
abstract::In 1981, the acquired immunodeficiency syndrome (AIDS) appeared insidiously and mystified doctors and scientists alike. No one could have predicted then that it would become, arguably, the worst plague in human history. Today, 33 million persons are living with infection by the human immunodeficiency virus type 1 (HIV...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.05.003
更新日期:2008-05-16 00:00:00
abstract::Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.01.015
更新日期:2010-01-22 00:00:00
abstract::In this issue of Cell, Korber et al. found that a SARS-CoV-2 variant in the spike protein D614G rapidly became dominant around the world. Although clinical and in vitro data suggest that D614G changes the virus phenotype, the impact of the mutation on transmission, disease, and vaccine and therapeutic development are ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2020.06.040
更新日期:2020-08-20 00:00:00
abstract::The molecular basis for membrane association of pp60v-src, the transforming protein of Rous sarcoma virus, was investigated in a cell-free system. Newly synthesized pp60v-src polypeptide, produced by in vitro translation of src mRNA, rapidly bound to plasma membranes. Binding was saturable and dependent on the presenc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90842-8
更新日期:1989-07-28 00:00:00
abstract::The emergence of SARS-CoV-2 has driven a global research effort to identify medical countermeasures at an unprecedented pace. In this issue of Cell, Cao et al. identify thousands of SARS-CoV-2 neutralizing antibodies from convalescent donors. The authors improve our understanding of immunity against the coronavirus sp...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2020.06.017
更新日期:2020-07-09 00:00:00
abstract::We have obtained the complete sequence of cloned full-length DNA (NS DNA) derived from influenza virus gene 8, which codes for two unique polypeptides, NS1 and NS2, and the sequence of the NS2 mRNA. Previously we showed that the mRNA for NS1 (approximately 860 nucleotides) is colinear with the viral RNA and maps from ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90484-5
更新日期:1980-09-01 00:00:00
abstract::Nonapoptotic forms of cell death may facilitate the selective elimination of some tumor cells or be activated in specific pathological states. The oncogenic RAS-selective lethal small molecule erastin triggers a unique iron-dependent form of nonapoptotic cell death that we term ferroptosis. Ferroptosis is dependent up...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.042
更新日期:2012-05-25 00:00:00
abstract::It has been puzzling that mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TIN2 have no recognized orthologs in budding yeast. Here, we describe a human protein, hRap1, that is an ortholog of the yeast telomeric protein, scRap1p. hRap1 has three conserved sequence motifs in common with scRap1, is loc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80858-2
更新日期:2000-05-26 00:00:00
abstract::We have studied the interaction of highly purified Int protein with DNA restriction fragments from the lambda phage attachment site (attP) region. Two different DNA sequences are protected by bound Int protein against partial digestion by either pancreatic DNAase or neocarzinostatin. One Int binding site includes the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90049-7
更新日期:1979-10-01 00:00:00
abstract::Twitter needs no introduction. This "microblogging" service has gained tremendous popularity in the 2 years since its launch. Yet, most scientists are steering clear of it. Laura Bonetta speaks to some who have found value in tweeting. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.017
更新日期:2009-10-30 00:00:00
abstract::The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.12.031
更新日期:2011-02-04 00:00:00