Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.

abstract：:Previous work from our laboratory has demonstrated that a subclass of the aminoglycoside antibiotics, those containing the drug fragment paromamine, stimulates mistranslation in cell-free protein-synthesizing systems derived from eucaryotic cells. We report here experiments which show that the ciliate Tetrahymena ther...

journal_title：Cell

authors： Palmer E,Wilhelm JM

更新日期：1978-02-01 00:00:00

abstract：:Large numbers of inbred laboratory rat strains have been developed for a range of complex disease phenotypes. To gain insights into the evolutionary pressures underlying selection for these phenotypes, we sequenced the genomes of 27 rat strains, including 11 models of hypertension, diabetes, and insulin resistance, al...

journal_title：Cell

authors： Atanur SS,Diaz AG,Maratou K,Sarkis A,Rotival M,Game L,Tschannen MR,Kaisaki PJ,Otto GW,Ma MC,Keane TM,Hummel O,Saar K,Chen W,Guryev V,Gopalakrishnan K,Garrett MR,Joe B,Citterio L,Bianchi G,McBride M,Dominiczak A

更新日期：2013-08-01 00:00:00

abstract：:Chromosomal translocations between antigen receptor loci and oncogenes are a hallmark of lymphoid cancers. Several new studies now reveal that programmed DNA breaks created during assembly of antigen receptor genes can be channeled into an alternative DNA end-joining pathway that is implicated in the chromosomal trans...

journal_title：Cell

authors： Nussenzweig A,Nussenzweig MC

更新日期：2007-10-19 00:00:00

abstract：:Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that have been primed for release by SNARE-complex assembly. Besides synaptotagmin 1, fast Ca(2+)-triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their ...

journal_title：Cell

authors： Tang J,Maximov A,Shin OH,Dai H,Rizo J,Südhof TC

更新日期：2006-09-22 00:00:00

abstract：:Mobile DNAs have had a central role in shaping our genome. More than half of our DNA is comprised of interspersed repeats resulting from replicative copy and paste events of retrotransposons. Although most are fixed, incapable of templating new copies, there are important exceptions to retrotransposon quiescence. De n...

journal_title：Cell

authors： Burns KH,Boeke JD

更新日期：2012-05-11 00:00:00

abstract：:We mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (neor) gene was introduced into an exon of a cloned fragment of the Hprt gene and used to transfect ES cells. Among the G418r col...

journal_title：Cell

authors： Thomas KR,Capecchi MR

更新日期：1987-11-06 00:00:00

abstract：:Reactive oxygen species (ROS) and mitochondrial defects in neurons are implicated in neurodegenerative disease. Here, we find that a key consequence of ROS and neuronal mitochondrial dysfunction is the accumulation of lipid droplets (LD) in glia. In Drosophila, ROS triggers c-Jun-N-terminal Kinase (JNK) and Sterol Reg...

journal_title：Cell

authors： Liu L,Zhang K,Sandoval H,Yamamoto S,Jaiswal M,Sanz E,Li Z,Hui J,Graham BH,Quintana A,Bellen HJ

更新日期：2015-01-15 00:00:00

abstract：:We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine resi...

journal_title：Cell

authors： Rijsewijk F,Schuermann M,Wagenaar E,Parren P,Weigel D,Nusse R

更新日期：1987-08-14 00:00:00

abstract：:DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2)...

journal_title：Cell

authors： Bohr VA,Smith CA,Okumoto DS,Hanawalt PC

更新日期：1985-02-01 00:00:00

abstract：:Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...

journal_title：Cell

authors： Ginsburg D

更新日期：2011-09-30 00:00:00

abstract：:Initiator tRNAs from the cytoplasm of salmon testes and salmon liver have been purified. The nucleotide sequence of these initiator tRNAs has been determined and found identical to that of initiator tRNA from mammalian cytoplasm. The only difference is the extent of modification of the nuceloside located between the d...

journal_title：Cell

authors： Gillum AM,Urquhart N,Smith M,RajBhandary UL

更新日期：1975-11-01 00:00:00

abstract：:Computational modeling and the theory of nonlinear dynamical systems allow one to not simply describe the events of the cell cycle, but also to understand why these events occur, just as the theory of gravitation allows one to understand why cannonballs fly in parabolic arcs. The simplest examples of the eukaryotic ce...

journal_title：Cell

authors： Ferrell JE Jr,Tsai TY,Yang Q

更新日期：2011-03-18 00:00:00

abstract：:Proteolysis is a key regulatory process that promotes the (in)activation, translocation, and/or degradation of proteins. As such, there is considerable interest in methods to comprehensively characterize proteolytic pathways in biological systems. Here, we describe a robust and versatile proteomic platform that enable...

journal_title：Cell

authors： Dix MM,Simon GM,Cravatt BF

更新日期：2008-08-22 00:00:00

abstract：:Tissue homeostasis requires maintenance of functional integrity under stress. A central source of stress is mechanical force that acts on cells, their nuclei, and chromatin, but how the genome is protected against mechanical stress is unclear. We show that mechanical stretch deforms the nucleus, which cells initially ...

journal_title：Cell

authors： Nava MM,Miroshnikova YA,Biggs LC,Whitefield DB,Metge F,Boucas J,Vihinen H,Jokitalo E,Li X,García Arcos JM,Hoffmann B,Merkel R,Niessen CM,Dahl KN,Wickström SA

更新日期：2020-05-14 00:00:00

abstract：:We have used cloned chicken cDNA sequences for alpha- and beta-tubulin to investigate tubulin gene organization in Drosophila melanogaster. Experiments using genomic Drosophila DNA from several sources indicate that there are at least four copies each of the alpha-tubulin gene and the beta-tubulin gene. In situ hybrid...

journal_title：Cell

authors： Sánchez F,Natzle JE,Cleveland DW,Kirschner MW,McCarthy BJ

更新日期：1980-12-01 00:00:00

abstract：:Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...

journal_title：Cell

authors： Eyler CE,Wu Q,Yan K,MacSwords JM,Chandler-Militello D,Misuraca KL,Lathia JD,Forrester MT,Lee J,Stamler JS,Goldman SA,Bredel M,McLendon RE,Sloan AE,Hjelmeland AB,Rich JN

更新日期：2011-07-08 00:00:00

abstract：:Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea ...

journal_title：Cell

authors： Matute DR,Butler IA,Coyne JA

更新日期：2009-12-11 00:00:00

abstract：:We have devised a genetic strategy to isolate the target of acidic activation domains of transcriptional activators based on toxicity in yeast cells of the chimeric activator, GAL4-VP16. Toxicity required the integrity of both the VP16 acidic activation domain and the GAL4 DNA-binding domain, suggesting that inhibitio...

journal_title：Cell

authors： Berger SL,Piña B,Silverman N,Marcus GA,Agapite J,Regier JL,Triezenberg SJ,Guarente L

更新日期：1992-07-24 00:00:00

abstract：:Major depressive disorder (MDD) patients display a common but often variable set of symptoms making successful, sustained treatment difficult to achieve. Separate depressive symptoms may be encoded by differential changes in distinct circuits in the brain, yet how discrete circuits underlie behavioral subsets of depre...

journal_title：Cell

authors： Knowland D,Lilascharoen V,Pacia CP,Shin S,Wang EH,Lim BK

更新日期：2017-07-13 00:00:00

abstract：:A model of hematopoietic development wherein multipotentiality is conserved until segregation of myeloid and lymphoid potential has recently been challenged, proposing that megakaryocyte/erythrocyte (MegE) potential is lost in Flk2/Flt3-expressing early progenitors. Here, we used sensitive in vivo approaches to quanti...

journal_title：Cell

authors： Forsberg EC,Serwold T,Kogan S,Weissman IL,Passegué E

更新日期：2006-07-28 00:00:00

abstract：:The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...

journal_title：Cell

authors： Shirayoshi Y,Okada TS,Takeichi M

更新日期：1983-12-01 00:00:00

abstract：:Actomyosin retrograde flow underlies the contraction essential for cell motility. Retrograde flow in both lamellipodia and lamella is required for membrane protrusion and for force generation by coupling to cell adhesion. We report that the Rac/Cdc42-binding kinase MRCK and myosin II-related MYO18A linked by the adapt...

journal_title：Cell

authors： Tan I,Yong J,Dong JM,Lim L,Leung T

更新日期：2008-10-03 00:00:00

abstract：:The Ras GTPase links extracellular mitogens to intracellular mechanisms that control cell proliferation. To understand how Ras regulates proliferation in vivo, we activated or inactivated Ras in cell clones in the developing Drosophila wing. Cells lacking Ras were smaller, had reduced growth rates, accumulated in G1, ...

journal_title：Cell

authors： Prober DA,Edgar BA

更新日期：2000-02-18 00:00:00

abstract：:Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis...

journal_title：Cell

authors： Flecken M,Wang H,Popilka L,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2020-10-15 00:00:00

abstract：:We have used confocal videomicroscopy in real time to delineate the adhesive interactions supporting platelet thrombus formation on biologically relevant surfaces. Type I collagen fibrils exposed to flowing blood adsorb von Willebrand factor (vWF), to which platelets become initially tethered with continuous surface t...

journal_title：Cell

authors： Savage B,Almus-Jacobs F,Ruggeri ZM

更新日期：1998-09-04 00:00:00

abstract：:Every day we make decisions critical for adaptation and survival. We repeat actions with known consequences. But we also draw on loosely related events to infer and imagine the outcome of entirely novel choices. These inferential decisions are thought to engage a number of brain regions; however, the underlying neuron...

journal_title：Cell

authors： Barron HC,Reeve HM,Koolschijn RS,Perestenko PV,Shpektor A,Nili H,Rothaermel R,Campo-Urriza N,O'Reilly JX,Bannerman DM,Behrens TEJ,Dupret D

更新日期：2020-10-01 00:00:00

abstract：:Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesives selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The re...

journal_title：Cell

authors： Nose A,Tsuji K,Takeichi M

更新日期：1990-04-06 00:00:00

abstract：:In contrast to uninfected chicken embryo fibroblasts (CEFs), CEFs infected with a retroviral vector that carries the v-erbA gene of avian erythroblastosis virus displayed new properties. These included limited anchorage-independent growth in soft agar, growth without latency in serum-supplemented medium, ability to ov...

journal_title：Cell

authors： Gandrillon O,Jurdic P,Benchaibi M,Xiao JH,Ghysdael J,Samarut J

更新日期：1987-06-05 00:00:00

abstract：:The glucocorticoid receptor is a signal transducer that interacts both with the signal and with the genes it regulates. We showed previously that nuclear localization of the receptor requires hormone binding. We have now constructed recombinant receptors that relieve hormonal control of nuclear localization, and we de...

journal_title：Cell

authors： Picard D,Salser SJ,Yamamoto KR

更新日期：1988-09-23 00:00:00

abstract：:The specificity of target gene activation by steroid receptors is encoded within a small, cysteine-rich domain that is believed to form two zinc-coordinated fingers. Here we show that the ability of glucocorticoid and estrogen receptors to discriminate between their closely related response elements resides in the two...

journal_title：Cell

authors： Danielsen M,Hinck L,Ringold GM

更新日期：1989-06-30 00:00:00