Abstract：
:The glucocorticoid receptor is a signal transducer that interacts both with the signal and with the genes it regulates. We showed previously that nuclear localization of the receptor requires hormone binding. We have now constructed recombinant receptors that relieve hormonal control of nuclear localization, and we demonstrate that the DNA binding/transcriptional regulatory functions of the receptor are also regulated directly by hormone. Surprisingly, regulation by the steroid binding domain appears to be relatively independent of protein structure. For example, regulation is maintained when the steroid binding region is repositioned from the C-terminus to the N-terminus of the receptor. Furthermore, the activity of an unrelated protein, the adenovirus E1A gene product, becomes hormone regulated upon fusion to the steroid binding domain. We speculate that the inhibitory effect of the unliganded steroid binding domain may be mediated by heat shock protein hsp90, which binds selectively to the unliganded receptor.
journal_name
Celljournal_title
Cellauthors
Picard D,Salser SJ,Yamamoto KRdoi
10.1016/0092-8674(88)90122-5subject
Has Abstractpub_date
1988-09-23 00:00:00pages
1073-80issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(88)90122-5journal_volume
54pub_type
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