Abstract:
:In contrast to uninfected chicken embryo fibroblasts (CEFs), CEFs infected with a retroviral vector that carries the v-erbA gene of avian erythroblastosis virus displayed new properties. These included limited anchorage-independent growth in soft agar, growth without latency in serum-supplemented medium, ability to overcome quiescence induced by serum deprivation, growth at low cell density, and an extended life span in vitro. Furthermore, when explanted in vivo onto the chorioallantoic membrane of chicken embryo, the transformed CEFs expressing v-erbA in addition to v-erbB exhibited a high proliferative rate, giving rise to fibrosarcoma tumors that were ten times larger than those developed from transformed CEFs expressing v-erbB alone. All these data show that CEFs expressing the v-erbA oncogene display activated growth and suggest that the v-erbA product interferes with the mechanisms regulating the growth and/or differentiation of primary CEFs.
journal_name
Celljournal_title
Cellauthors
Gandrillon O,Jurdic P,Benchaibi M,Xiao JH,Ghysdael J,Samarut Jdoi
10.1016/0092-8674(87)90545-9subject
Has Abstractpub_date
1987-06-05 00:00:00pages
687-97issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(87)90545-9journal_volume
49pub_type
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