Abstract:
:Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
journal_name
Celljournal_title
Cellauthors
Stelzl U,Worm U,Lalowski M,Haenig C,Brembeck FH,Goehler H,Stroedicke M,Zenkner M,Schoenherr A,Koeppen S,Timm J,Mintzlaff S,Abraham C,Bock N,Kietzmann S,Goedde A,Toksöz E,Droege A,Krobitsch S,Korn B,Birchmeier W,doi
10.1016/j.cell.2005.08.029subject
Has Abstractpub_date
2005-09-23 00:00:00pages
957-68issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(05)00866-4journal_volume
122pub_type
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