Abstract：
:The general inhibition in transcriptional activity during mitosis abolishes the stress-inducible expression of the human hsp70 gene. Among the four transcription factors that bind to the human hsp70 promoter, the DNA-binding activities of three (C/EBP, GBP, and HSF1) were normal, while Sp1 showed reduced binding activity in mitotic cell extracts. In vivo footprinting and immunocytochemical analyses revealed that all of the sequence-specific transcription factors were displaced from promoter sequences as well as from bulk chromatin during mitosis. The correlation of transcription factor displacement with chromatin condensation suggests an involvement of chromatin structure in mitotic repression. However, retention of DNase I hypersensitivity suggests that the hsp70 promoter was not organized in a canonical nucleosome structure in mitotic chromatin. Displacement of transcription factors from mitotic chromosomes could present another window in the cell cycle for resetting transcriptional programs.
journal_name
Celljournal_title
Cellauthors
Martínez-Balbás MA,Dey A,Rabindran SK,Ozato K,Wu Cdoi
10.1016/0092-8674(95)90231-7subject
Has Abstractpub_date
1995-10-06 00:00:00pages
29-38issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(95)90231-7journal_volume
83pub_type
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