Abstract：
:The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and the isoleucine are tightly bound by two well-defined pockets on the protein surface, resulting in a complex that resembles a two-pronged plug engaging a two-holed socket. The glutamate residues are in solvent-exposed environments in the vicinity of basic side chains of the SH2 domain, and the two N-terminal residues cap the phosphotyrosine-binding site. The crystal structure of Src SH2 in the absence of peptide has been determined at 2.5 A resolution, and comparison with the structure of the high affinity complex reveals only localized and relatively small changes.
journal_name
Celljournal_title
Cellauthors
Waksman G,Shoelson SE,Pant N,Cowburn D,Kuriyan Jdoi
10.1016/0092-8674(93)90405-fsubject
Has Abstractpub_date
1993-03-12 00:00:00pages
779-90issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(93)90405-Fjournal_volume
72pub_type
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