Abstract:
:Drosophila bicoid mRNA is synthesized in the nurse cells and transported to the oocyte where microtubules and Exuperantia protein mediate localization to the anterior pole. Fluorescent bicoid mRNA injected into the oocyte displays nonpolar microtubule-dependent transport to the closest cortical surface, and the oocyte microtubule cytoskeleton lacks clear axial asymmetry. Nonetheless, bicoid mRNA injected into the nurse cell cytoplasm, withdrawn, and injected into a second oocyte shows microtubule-dependent transport to the anterior cortex. Nurse cells require microtubules and Exuperantia to support anterior transport of bicoid mRNA, and microtubules are required for bicoid mRNA-Exuperantia particle coassembly. We propose that microtubule-dependent Exuperantia-bicoid mRNA complex formation in the nurse cell cytoplasm allows anterior-specific transport on a grossly nonpolar oocyte microtubule network.
journal_name
Celljournal_title
Cellauthors
Cha BJ,Koppetsch BS,Theurkauf WEdoi
10.1016/s0092-8674(01)00419-6subject
Has Abstractpub_date
2001-07-13 00:00:00pages
35-46issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(01)00419-6journal_volume
106pub_type
杂志文章相关文献
CELL文献大全abstract::Polyglycylation is a posttranslational modification that generates glycine side chains on proteins. Here we identify a family of evolutionarily conserved glycine ligases that modify tubulin using different enzymatic mechanisms. In mammals, two distinct enzyme types catalyze the initiation and elongation steps of polyg...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.05.020
更新日期:2009-06-12 00:00:00
abstract::Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.06.036
更新日期:2006-07-28 00:00:00
abstract::Interactions between similar but not identical (homeologous) DNA sequences play an important biological role in the evolution of genes and genomes. To gain insight into the underlying molecular mechanism(s) of genetic recombination, we have studied inter- and intramolecular homeologous recombination in S. cerevisiae d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90434-e
更新日期:1992-08-21 00:00:00
abstract::Most mutations in cancer genomes are thought to be acquired after the initiating event, which may cause genomic instability and drive clonal evolution. However, for acute myeloid leukemia (AML), normal karyotypes are common, and genomic instability is unusual. To better understand clonal evolution in AML, we sequenced...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.06.023
更新日期:2012-07-20 00:00:00
abstract::In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.057
更新日期:2018-11-15 00:00:00
abstract::We have determined the complete 9202 nucleotide sequence of the visna lentivirus. The deduced genetic organization most closely resembles that of the AIDS retrovirus in that there is a novel central region separating pol and env. Moreover, there is a close phylogenetic relationship between the conserved reverse transc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80132-x
更新日期:1985-08-01 00:00:00
abstract::The synthesis of histones and DNA was examined in BHK cells arrested in G1 by isoleucine starvation and in cells progressing into the S phase upon isoleucine refeeding. Approximately 2-3% of the cells were not arrested in G1 and synthesized DNA. The rate of synthesis of DNA and nucleosomal histones observed in cells s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90092-2
更新日期:1978-09-01 00:00:00
abstract::The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81517-2
更新日期:1999-09-17 00:00:00
abstract::In many organisms, meiotic chromosomes are bundled at their telomeres to form a "bouquet" arrangement. The bouquet formation plays an important role in homologous chromosome pairing and therefore progression of meiosis. As meiotic telomere clustering occurs in response to mating pheromone signaling in fission yeast, w...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.01.048
更新日期:2006-04-07 00:00:00
abstract::Negative feedback is a widespread feature of signaling pathways. In an unexpected twist described in this issue, He and colleagues identify a membrane-tethered metalloprotease named Tiki that inhibits Wnt signaling by removing an essential eight-residue fragment from Wnt itself. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.06.007
更新日期:2012-06-22 00:00:00
abstract::At early times after adenovirus infection, transcription from all early viral promoters requires the expression of a 289 amino acid phosphoprotein encoded in the pre-early transcription unit E1A. Mutations in E1A result in a marked delay in the induction of early viral transcription. To distinguish between possible mo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90011-9
更新日期:1983-07-01 00:00:00
abstract::Polycomb group (PcG) proteins form large multimeric complexes (PcG bodies) which are involved in the stable repression of gene expression. The human PcG protein, Pc2, has been shown to recruit the transcriptional corepressor, CtBP, to PcG bodies. We show that CtBP is sumoylated at a single lysine. In vitro, CtBP sumoy...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00159-4
更新日期:2003-04-04 00:00:00
abstract::Rat liver interphase chromosomes have telomeres 20-100 kb in length. Micrococcal nuclease digestion of nuclei cleaves telomeres with a uniform 157 bp periodicity, producing soluble particles that sediment in sucrose gradients exactly like oligonucleosomes. The monomeric telomere particles comigrate with nucleosome cor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90256-p
更新日期:1993-05-21 00:00:00
abstract::The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. We have solved the X-ray crystal structure to 1.9 A resolution of an autoinhibited, unphosphorylated form of EphB2 comprised of the juxtamembrane region and the kinase domain. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00496-2
更新日期:2001-09-21 00:00:00
abstract::A study in this issue of Cell illustrates the power of applying genomic approaches with model systems to characterize the biological activity of small molecules and to identify their cellular targets, which can clarify the mode of action of human therapeutics. ...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s0092-8674(03)01070-5
更新日期:2004-01-09 00:00:00
abstract::Highly virulent influenza virus infection results in excessive cytokine production, recruitment of leukocytes, and immune-mediated pulmonary injury. Teijaro et al. (2011) now demonstrate that sphingosine-1-phosphate receptor 1 ligands suppress all features of flu-inflicted pathological inflammation and place the endot...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2011.08.027
更新日期:2011-09-16 00:00:00
abstract::Synaptotagmin is an abundant synaptic vesicle-associated protein proposed to be involved in calcium-mediated neurotransmitter release. Our molecular and genetic results demonstrate that, although synaptotagmin is required for the proper function of the presynaptic nerve terminal in C. elegans, some neurotransmitter re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90357-v
更新日期:1993-07-02 00:00:00
abstract::Reactive oxygen species (ROS) and mitochondrial defects in neurons are implicated in neurodegenerative disease. Here, we find that a key consequence of ROS and neuronal mitochondrial dysfunction is the accumulation of lipid droplets (LD) in glia. In Drosophila, ROS triggers c-Jun-N-terminal Kinase (JNK) and Sterol Reg...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.12.019
更新日期:2015-01-15 00:00:00
abstract::Microtubule-dependent motor, murine KIF3B, was disrupted by gene targeting. The null mutants did not survive beyond midgestation, exhibiting growth retardation, pericardial sac ballooning, and neural tube disorganization. Prominently, the left-right asymmetry was randomized in the heart loop and the direction of embry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81705-5
更新日期:1998-12-11 00:00:00
abstract::Poly(A)-containing RNA, isolated from rat ventral prostate, has been analyzed for its base sequence complexity. The kinetics of hybridization of total cellular poly(A)-containing RNA with its complementary DNA (cDNA) from normal and castrated animals are markedly different. RNA from normal animals consists of three ab...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90116-7
更新日期:1977-10-01 00:00:00
abstract::Following DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumpti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.08.008
更新日期:2013-08-29 00:00:00
abstract::Actomyosin retrograde flow underlies the contraction essential for cell motility. Retrograde flow in both lamellipodia and lamella is required for membrane protrusion and for force generation by coupling to cell adhesion. We report that the Rac/Cdc42-binding kinase MRCK and myosin II-related MYO18A linked by the adapt...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.09.018
更新日期:2008-10-03 00:00:00
abstract::Escherichia coli and Micrococcus luteus DNA topoisomerase I are found to promote catenation of double-stranded DNA rings. At low DNA concentration dimeric catenanes are the major catenated products; at high DNA concentration or when spermidine is present, catenanes containing more than two rings are formed. There is n...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90174-9
更新日期:1980-11-01 00:00:00
abstract::Members of the E2F transcription factor family (E2F-1-E2F-5) are believed to be critical positive regulators of cell cycle progression in eukaryotes although the in vivo functions of the individual E2Fs have not been elucidated. Mice were generated that lack E2F-1 and, surprisingly, these mice develop and reproduce no...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81255-6
更新日期:1996-05-17 00:00:00
abstract::The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90604-1
更新日期:1989-12-01 00:00:00
abstract::The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81732-8
更新日期:1998-09-18 00:00:00
abstract::Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90197-m
更新日期:1990-06-01 00:00:00
abstract::Dopamine plays an important role in the etiology of schizophrenia, and D2 class dopamine receptors are the best-established target of antipsychotic drugs. Here we show that D2 class-receptor-mediated Akt regulation involves the formation of signaling complexes containing beta-arrestin 2, PP2A, and Akt. beta-arrestin 2...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.05.012
更新日期:2005-07-29 00:00:00
abstract::Clones containing five non-adult beta-globin genes were isolated from a library of BALB/c DNA. Together, the newly cloned regions comprise a contiguous block of 32 kb of the mouse genome. Restriction mapping of genomic DNA established the physical linkage of these non-adult genes to the two adult beta-globin genes as ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90123-3
更新日期:1980-08-01 00:00:00
abstract::Using a combination of classical genetics, serology, and molecular genetics, we have mapped as many as possible of the MHC components in t haplotypes. Results indicate that the whole MHC is included in a simple inversion with the relative positions of its genes being intact. Furthermore, some of the t-lethal mutations...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90464-1
更新日期:1984-12-01 00:00:00