Abstract：
:Actomyosin retrograde flow underlies the contraction essential for cell motility. Retrograde flow in both lamellipodia and lamella is required for membrane protrusion and for force generation by coupling to cell adhesion. We report that the Rac/Cdc42-binding kinase MRCK and myosin II-related MYO18A linked by the adaptor protein LRAP35a form a functional tripartite complex, which is responsible for the assembly of lamellar actomyosin bundles and of a subnuclear actomyosin network. LRAP35a binds independently to MYO18A and MRCK. This binding leads to MRCK activation and its phosphorylation of MYO18A, independently of ROK and MLCK. The MRCK complex moves in concert with the retrograde flow of actomyosin bundles, with MRCK being able to influence other flow components such as MYO2A. The promotion of persistent protrusive activity and inhibition of cell motility by the respective expression of wild-type and dominant-negative mutant components of the MRCK complex show it to be crucial to cell protrusion and migration.
journal_name
Celljournal_title
Cellauthors
Tan I,Yong J,Dong JM,Lim L,Leung Tdoi
10.1016/j.cell.2008.09.018subject
Has Abstractpub_date
2008-10-03 00:00:00pages
123-36issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(08)01140-9journal_volume
135pub_type
杂志文章相关文献
CELL文献大全abstract：:The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.10.009
更新日期：2008-10-31 00:00:00
abstract：:Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.10.008
更新日期：2015-11-05 00:00:00
abstract：:Apoptosis and autophagy are both tightly regulated biological processes that play a central role in tissue homeostasis, development, and disease. The anti-apoptotic protein, Bcl-2, interacts with the evolutionarily conserved autophagy protein, Beclin 1. However, little is known about the functional significance of thi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.07.002
更新日期：2005-09-23 00:00:00
abstract：:Mitochondrial DNA is compacted into nucleoprotein complexes denoted mitochondrial nucleoids, the focus of this SnapShot. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2017.12.039
更新日期：2018-01-11 00:00:00
abstract：:At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.03.023
更新日期：2012-04-13 00:00:00
abstract：:In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to date, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Over...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81819-x
更新日期：1996-12-27 00:00:00
abstract：:The elucidation of the genetic code remains among the most influential discoveries in biology. While innumerable studies have validated the general universality of the code and its value in predicting and analyzing protein coding sequences, established and emerging work has also suggested that full genome decryption m...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.09.022
更新日期：2016-12-15 00:00:00
abstract：:A large (approximately 200 kd) subunit of cellular RNA polymerase II (Pol II) and a virus-encoded subunit of rabbit poxvirus (RPV) RNA polymerase (137 kd) react with common monoclonal antibodies. Hybridization studies with viral and cellular DNA clones confirm that the viral and cellular proteins are related. Followin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90268-0
更新日期：1986-02-28 00:00:00
abstract：:The copy number of yeast histone genes was increased by inserting an extra H2A,H2B gene pair into the haploid genome by the technique of yeast transformation. The presence of this extra gene copy has no detectable effect on cell growth. The steady-state levels of histone H2A,H2B mRNAs are not elevated in transformed s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90327-5
更新日期：1981-05-01 00:00:00
abstract：:Proteins, particularly viral proteins, can be multifunctional, but the mechanisms behind multifunctionality are not fully understood. Here, we illustrate through multiple crystal structures, biochemistry, and cellular microscopy that VP40 rearranges into different structures, each with a distinct function required for...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.07.015
更新日期：2013-08-15 00:00:00
abstract：:Elements controlling tissue-specific expression of the rat albumin gene reside within roughly 150 bp upstream of the transcriptional initiation site. We show here by DNAase I footprinting assays that at least four distinct factors present in extracts derived from cells expressing albumin interact with these sequences....
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90036-5
更新日期：1987-08-14 00:00:00
abstract：:Monkey cell lines that constitutively synthesize 38.6 kd lac repressor protein and bear stably integrated chloramphenicol acetyl transferase (CAT) genes linked to a lac operator-containing SV40 early promoter-enhancer were generated. When grown in medium containing isopropyl beta-D-thiogalactoside (IPTG), these cells ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90409-6
更新日期：1988-03-11 00:00:00
abstract：:We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90432-x
更新日期：1984-07-01 00:00:00
abstract：:The 2.2 kb mRNA of the Dictyostelium discoideum prespore gene EB4-PSV is constitutively transcribed during growth and development, but the message is only accumulated when cells form aggregates and establish the prespore-prestalk pattern. Disruption of the pattern by mechanical disaggregation results in a rapid loss o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90130-5
更新日期：1992-04-03 00:00:00
abstract：:The in vitro folding pathway of bovine pancreatic trypsin inhibitor (BPTI) has been described previously in terms of the disulfide-bonded intermediates that accumulate during folding of the protein. Folding is slow, occurring in hours at pH 7.3, 25 degrees C. In addition, approximately half of the BPTI molecules becom...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90559-u
更新日期：1992-11-27 00:00:00
abstract：:Neuroinflammation is being increasingly recognized as a potential mediator of cognitive impairments in various neurological conditions. Habbas et al. demonstrate that the pro-inflammatory cytokine tumor necrosis factor alpha signals through astrocytes to alter synaptic transmission and impair cognition in a mouse mode...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.12.001
更新日期：2015-12-17 00:00:00
abstract：:Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90670-a
更新日期：1990-02-23 00:00:00
abstract：:The membrane-bound form of immunoglobulin serves as an antigen-specific receptor for B cells mediating signal transduction and antigen presentation. We have developed an assay that reconstitutes both these physiologic responses with respect to the antigen phosphorylcholine. By introducing specific mutations in the hum...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90171-a
更新日期：1990-10-19 00:00:00
abstract：:Accumulating evidence for nascent-peptide-mediated regulation of translation suggests that all nascent peptides do not necessarily interact with the ribosome in a similar manner. Recent studies have helped to elucidate the exit route of the nascent chain and its interactions with the ribosome. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00669-4
更新日期：2002-03-08 00:00:00
abstract：:The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by lon...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90147-3
更新日期：1985-02-01 00:00:00
abstract：:The molecular mediator and functional significance of meal-associated brown fat (BAT) thermogenesis remains elusive. Here, we identified the gut hormone secretin as a non-sympathetic BAT activator mediating prandial thermogenesis, which consequentially induces satiation, thereby establishing a gut-secretin-BAT-brain a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.016
更新日期：2018-11-29 00:00:00
abstract：:Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.06.022
更新日期：2020-08-06 00:00:00
abstract：:Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized pr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90425-7
更新日期：1983-06-01 00:00:00
abstract：:Erythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81785-7
更新日期：1998-10-02 00:00:00
abstract：:Activation of the transcription factor NF-kappaB by tumor necrosis factor (TNF) and interleukin-1 (IL-1) requires the NF-kappaB-inducing kinase (NIK). In a yeast two-hybrid screen for NIK-interacting proteins, we have identified a protein kinase previously known as CHUK. Overexpression of CHUK activates a NF-kappaB-de...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80344-x
更新日期：1997-07-25 00:00:00
abstract：:We have shown that lariat formation during in vitro splicing of several RNA precursors, from Drosophila to man, occurs at a unique and identifiable but weakly conserved site, 18 to 37 nucleotides proximal to the 3' splice site. Lariat formation within an artificial intron lacking a normal branch-point sequence occurs ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90064-9
更新日期：1985-05-01 00:00:00
abstract：:In Drosophila melanogaster males, the sex chromosomes pair during meiosis in the centric X heterochromatin and at the base of the short arm of the Y (YS), in the vicinity of the nucleolus organizers. X chromosomes deficient for the pairing region segregate randomly from the Y. In this report we show that a single ribo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90215-z
更新日期：1990-04-06 00:00:00
abstract：:D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. We tested the requirement for D-cyclins in mouse development and in proliferation by generating mice lacking all D-cyclins. We found that these cyclin D1(-/-)D2(-/-)D3(-/-) mice develop u...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.07.025
更新日期：2004-08-20 00:00:00
abstract：:The murine major histocompatibility complex I region encodes two class II antigens, I-A and I-E. From a mouse spleen DNA cosmid library of the b haplotype, we isolated a clone containing the entire I-A beta gene and a separate exon encoding a beta-chain second domain (A beta 2). The A beta gene, encompassing more than...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90148-4
更新日期：1983-08-01 00:00:00
abstract：:Immune responses involve coordination across cell types and tissues. However, studies in cancer immunotherapy have focused heavily on local immune responses in the tumor microenvironment. To investigate immune activity more broadly, we performed an organism-wide study in genetically engineered cancer models using mass...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.12.022
更新日期：2017-01-26 00:00:00
