Abstract:
:Monkey cell lines that constitutively synthesize 38.6 kd lac repressor protein and bear stably integrated chloramphenicol acetyl transferase (CAT) genes linked to a lac operator-containing SV40 early promoter-enhancer were generated. When grown in medium containing isopropyl beta-D-thiogalactoside (IPTG), these cells acquired a CAT+ phenotype. In contrast, when grown in parallel in medium lacking IPTG, the cells remained CAT-. Maximum induction of CAT activity occurred after 4 days of IPTG exposure. Three days after removal of IPTG, induced cells had reverted to CAT-. Specific CAT activity increased up to 60-fold after induction, while background activity in uninduced cells was similar to or only slightly above that of parental, CAT- cells. CAT activity increased stepwise over a wide range of IPTG concentrations. Thus lac repressor-operator complexes can form on primate chromosomes and stringently block transcription from an adjoining promoter.
journal_name
Celljournal_title
Cellauthors
Figge J,Wright C,Collins CJ,Roberts TM,Livingston DMdoi
10.1016/0092-8674(88)90409-6subject
Has Abstractpub_date
1988-03-11 00:00:00pages
713-22issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(88)90409-6journal_volume
52pub_type
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