Abstract：
:Transposon mutagenesis and complementation analysis of the photosynthesis genes in Rhodopseudomonas capsulata is presented utilizing Tn5.7 mutagenized R-primes. The R-prime pRPS404 contains many of the genes necessary for the differentiation of the photosynthetic apparatus. Utilizing homologous recombination, 30 independent copies of Tn5.7 were inserted into the R. capsulata chromosome with subsequent deletion of wild-type alleles. Mutants were characterized by absorption spectroscopy, SDS-PAGE, and determination of capability for photosynthetic growth. Many mutations in the bacteriochlorophyll and carotenoid biosynthetic pathways were isolated. A regulatory mutation was isolated affecting reaction-center synthesis as well as a 44 kd heme-containing polypeptide. Complementation analysis using various pRPR404::Tn5.7 plasmids has led to the postulation of transcriptional units.
journal_name
Celljournal_title
Cellauthors
Zsebo KM,Hearst JEdoi
10.1016/0092-8674(84)90428-8subject
Has Abstractpub_date
1984-07-01 00:00:00pages
937-47issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(84)90428-8journal_volume
37pub_type
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