Specific and saturable binding of pp60v-src to plasma membranes: evidence for a myristyl-src receptor.


:The molecular basis for membrane association of pp60v-src, the transforming protein of Rous sarcoma virus, was investigated in a cell-free system. Newly synthesized pp60v-src polypeptide, produced by in vitro translation of src mRNA, rapidly bound to plasma membranes. Binding was saturable and dependent on the presence of myristate at the amino terminus of pp60v-src. Prior treatment of membranes with heat or trypsin greatly decreased subsequent binding of pp60v-src. Membrane binding of pp60v-src was competed by a myristylated peptide containing the first 11 amino acids of the mature src sequence, but not by non-myristylated src peptide or other myristylated peptides. The specificity, saturability, and competitive nature of pp60v-src binding provide evidence for the existence of a src receptor in the plasma membrane.






Resh MD




Has Abstract


1989-07-28 00:00:00
















  • Single-Molecule Visualization of MCM2-7 DNA Loading: Seeing Is Believing.

    abstract::The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...


    pub_type: 评论,杂志文章


    authors: Chistol G,Walter JC

    更新日期:2015-04-23 00:00:00

  • Nucleosome arrangement on tRNA genes of Xenopus laevis.

    abstract::We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly sp...


    pub_type: 杂志文章


    authors: Bryan PN,Hofstetter H,Birnstiel ML

    更新日期:1981-12-01 00:00:00

  • Human transposon tectonics.

    abstract::Mobile DNAs have had a central role in shaping our genome. More than half of our DNA is comprised of interspersed repeats resulting from replicative copy and paste events of retrotransposons. Although most are fixed, incapable of templating new copies, there are important exceptions to retrotransposon quiescence. De n...


    pub_type: 杂志文章,评审


    authors: Burns KH,Boeke JD

    更新日期:2012-05-11 00:00:00

  • Integrin alpha8beta1 is critically important for epithelial-mesenchymal interactions during kidney morphogenesis.

    abstract::We present genetic evidence that integrins regulate epithelial-mesenchymal interactions during organogenesis. Mice with a mutation in the alpha8 gene do not express the integrin alpha8 beta1 and exhibit profound deficits in kidney morphogenesis. In wild-type animals, inductive interactions between the ureteric epithel...


    pub_type: 杂志文章


    authors: Müller U,Wang D,Denda S,Meneses JJ,Pedersen RA,Reichardt LF

    更新日期:1997-03-07 00:00:00

  • Acetylation is indispensable for p53 activation.

    abstract::The activation of the tumor suppressor p53 facilitates the cellular response to genotoxic stress; however, the p53 response can only be executed if its interaction with its inhibitor Mdm2 is abolished. There have been conflicting reports on the question of whether p53 posttranslational modifications, such as phosphory...


    pub_type: 杂志文章


    authors: Tang Y,Zhao W,Chen Y,Zhao Y,Gu W

    更新日期:2008-05-16 00:00:00

  • GABAAR Modulator for Postpartum Depression.

    abstract::During the postpartum period, the brain's inhibitory GABAA receptors may not recover in time following their reduced numbers during pregnancy. This is likely the cause of postpartum depression prevalent in ∼12% of childbearing women. A new therapy for this condition consists of administering a synthetic neurosteroid d...


    pub_type: 杂志文章


    authors: Mody I

    更新日期:2019-01-10 00:00:00

  • IKKalpha at the crossroads of inflammation and metastasis.

    abstract::The development of solid tumors is regulated by dynamic interactions between evolving neoplastic cells and their microenvironment. Luo et al. (2007) recently demonstrated that tumor-infiltrating immune cells expressing RANKL induce activation and nuclear localization of IKKalpha in prostatic epithelial tumor cells. Th...


    pub_type: 杂志文章


    authors: Affara NI,Coussens LM

    更新日期:2007-04-06 00:00:00

  • Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.

    abstract::Histone DNA of Psammechinus miliaris was obtained in an enriched form by buoyant density gradient centrifugation and was cleaved into 6 kb repeat units (Birnstiel et al., 1975a) by the action of the specific endonucleases EcoRI and HindIII. Since it was suspected that the 6 kb unit harbored all five histone-coding seq...


    pub_type: 杂志文章


    authors: Schaffner W,Gross K,Telford J,Birnstiel M

    更新日期:1976-08-01 00:00:00

  • Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity.

    abstract::Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on t...


    pub_type: 杂志文章


    authors: Sen J,Goltz JS,Stevens L,Stein D

    更新日期:1998-11-13 00:00:00

  • Solving mysteries of DNA replication and frog cloning.

    abstract::Compared to sperm nuclei, nuclei from adult somatic cells replicate inefficiently in frog egg extract. In this issue of Cell, Lemaitre et al. (2005) show that pre-exposure of erythrocyte nuclei to a mitotic extract removes this difference, reorganizes the chromatin into shorter loops, and allows replication at much sh...


    pub_type: 评论,杂志文章


    authors: Laskey R

    更新日期:2005-12-02 00:00:00

  • Neuronal Computation Underlying Inferential Reasoning in Humans and Mice.

    abstract::Every day we make decisions critical for adaptation and survival. We repeat actions with known consequences. But we also draw on loosely related events to infer and imagine the outcome of entirely novel choices. These inferential decisions are thought to engage a number of brain regions; however, the underlying neuron...


    pub_type: 杂志文章


    authors: Barron HC,Reeve HM,Koolschijn RS,Perestenko PV,Shpektor A,Nili H,Rothaermel R,Campo-Urriza N,O'Reilly JX,Bannerman DM,Behrens TEJ,Dupret D

    更新日期:2020-10-01 00:00:00

  • Need for Speed: From Human SARS-CoV-2 Samples to Protective and Efficacious Antibodies in Weeks.

    abstract::The emergence of SARS-CoV-2 has driven a global research effort to identify medical countermeasures at an unprecedented pace. In this issue of Cell, Cao et al. identify thousands of SARS-CoV-2 neutralizing antibodies from convalescent donors. The authors improve our understanding of immunity against the coronavirus sp...


    pub_type: 评论,杂志文章


    authors: Joyce MG,Wheatley AK,Modjarrad K

    更新日期:2020-07-09 00:00:00

  • Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene.

    abstract::In cells of the B-lymphocyte lineage, 8.4 kb transcripts are constitutively produced from unrearranged kappa constant region (kappa 0) loci. To help elucidate the molecular basis of this phenomenon, we have determined the nucleotide sequence surrounding the site of transcriptional initiation. The kappa 0 transcripts a...


    pub_type: 杂志文章


    authors: Van Ness BG,Weigert M,Coleclough C,Mather EL,Kelley DE,Perry RP

    更新日期:1981-12-01 00:00:00

  • Exposing p120 catenin's most intimate affair.

    abstract::p120-catenin regulates cell-cell adhesion by controlling cell surface retention of cadherin. In this issue, Ishiyama et al. (2010) present the first crystal structure of p120 in complex with cadherin, revealing molecular details of the functional interface and providing sophisticated new tools for dissecting p120's ro...


    pub_type: 评论,杂志文章


    authors: Reynolds AB

    更新日期:2010-04-02 00:00:00

  • Extended self-renewal capacity of pluripotent hemopoietic stem cells: association with persistent Friend spleen focus-forming virus.

    abstract::The pluripotential hemopoietic stem cells (CFU-S) of genetically resistant Fv2rr adult C57BL/6 (B6) mice can be persistently infected by Friend virus (FV) when exposed to defective spleen focus-forming virus (SFFV) and helper virus (LLV) at the time of transplantation into heavily irradiated syngeneic recipients (that...


    pub_type: 杂志文章


    authors: Eckner RJ,Hettrick KL,Greenberger JS,Bennett M

    更新日期:1982-12-01 00:00:00

  • Scrambled ribosomal RNA gene pieces in Chlamydomonas reinhardtii mitochondrial DNA.

    abstract::We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...


    pub_type: 杂志文章


    authors: Boer PH,Gray MW

    更新日期:1988-11-04 00:00:00

  • A nonpolymorphic class I gene in the murine major histocompatibility complex.

    abstract::DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/10 (H-2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library. Exon 5, in particular, has an unusual structure such that a polypeptide produc...


    pub_type: 杂志文章


    authors: Mellor AL,Weiss EH,Kress M,Jay G,Flavell RA

    更新日期:1984-01-01 00:00:00

  • Function of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing requires an idiosyncratic domain not found in other synthetases.

    abstract::Neurospora mitochondrial tyrosyl-tRNA synthetase (mt TyrRS), which is encoded by nuclear gene cyt-18, functions in splicing group I introns. Analysis of intragenic partial revertants of the cyt-18-2 mutant and in vitro mutants of the cyt-18 protein expressed in E. coli showed that splicing activity of the cyt-18 prote...


    pub_type: 杂志文章


    authors: Cherniack AD,Garriga G,Kittle JD Jr,Akins RA,Lambowitz AM

    更新日期:1990-08-24 00:00:00

  • A role for recombination junctions in the segregation of mitochondrial DNA in yeast.

    abstract::In S. cerevisiae, mitochondrial DNA (mtDNA) molecules, in spite of their high copy number, segregate as if there were a small number of heritable units. The rapid segregation of mitochondrial genomes can be analyzed using mtDNA deletion variants. These small, amplified genomes segregate preferentially from mixed zygot...


    pub_type: 杂志文章


    authors: Lockshon D,Zweifel SG,Freeman-Cook LL,Lorimer HE,Brewer BJ,Fangman WL

    更新日期:1995-06-16 00:00:00

  • Hippo pathway activity influences liver cell fate.

    abstract::The Hippo-signaling pathway is an important regulator of cellular proliferation and organ size. However, little is known about the role of this cascade in the control of cell fate. Employing a combination of lineage tracing, clonal analysis, and organoid culture approaches, we demonstrate that Hippo pathway activity i...


    pub_type: 杂志文章


    authors: Yimlamai D,Christodoulou C,Galli GG,Yanger K,Pepe-Mooney B,Gurung B,Shrestha K,Cahan P,Stanger BZ,Camargo FD

    更新日期:2014-06-05 00:00:00

  • Translation silenced by fused pair of tRNA synthetases.

    abstract::In this issue of Cell, discover gene-specific translational silencing as a novel function of the fused glutamyl- and prolyl-tRNA synthetase (GluProRS). GluProRS is released from a multisynthetase translation complex in response to gamma-interferon and forms a four-protein GAIT complex that silences translation of ceru...


    pub_type: 评论,杂志文章


    authors: Schimmel P,Ewalt K

    更新日期:2004-10-15 00:00:00

  • "Wunder" F-BAR domains: going from pits to vesicles.

    abstract::Clathrin-mediated endocytosis is a key mechanism by which cells take up extracellular cargo. In this issue, Shimada et al. (2007) reveal the mode of action of the F-BAR domain, which deepens the initial membrane pit that forms during clathrin-mediated endocytosis. ...


    pub_type: 评论,杂志文章,评审


    authors: Fütterer K,Machesky LM

    更新日期:2007-05-18 00:00:00

  • Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

    abstract::Transfer RNAs (tRNAs) are primarily viewed as static contributors to gene expression. By developing a high-throughput tRNA profiling method, we find that specific tRNAs are upregulated in human breast cancer cells as they gain metastatic activity. Through loss-of-function, gain-of-function, and clinical-association st...


    pub_type: 杂志文章


    authors: Goodarzi H,Nguyen HCB,Zhang S,Dill BD,Molina H,Tavazoie SF

    更新日期:2016-06-02 00:00:00

  • Transcription initiation at its most basic level.

    abstract::The structure of the initiator binding protein from the primitive eukaryote Trichomonas vaginalis reveals how a single protein can orchestrate promoter recognition, RNA polymerase II recruitment, and start site selection, and provides an evolutionary link with transcription initiation in higher eukaryotes. ...


    pub_type: 评论,杂志文章


    authors: Marmorstein R

    更新日期:2003-11-14 00:00:00

  • The lck tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique amino-terminal domain.

    abstract::The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...


    pub_type: 杂志文章


    authors: Shaw AS,Amrein KE,Hammond C,Stern DF,Sefton BM,Rose JK

    更新日期:1989-11-17 00:00:00

  • Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

    abstract::We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule...


    pub_type: 杂志文章


    authors: Lee JO,Rieu P,Arnaout MA,Liddington R

    更新日期:1995-02-24 00:00:00

  • Ragulator is a GEF for the rag GTPases that signal amino acid levels to mTORC1.

    abstract::The mTOR Complex 1 (mTORC1) pathway regulates cell growth in response to numerous cues, including amino acids, which promote mTORC1 translocation to the lysosomal surface, its site of activation. The heterodimeric RagA/B-RagC/D GTPases, the Ragulator complex that tethers the Rags to the lysosome, and the v-ATPase form...


    pub_type: 杂志文章


    authors: Bar-Peled L,Schweitzer LD,Zoncu R,Sabatini DM

    更新日期:2012-09-14 00:00:00

  • The crystal structure of HaeIII methyltransferase convalently complexed to DNA: an extrahelical cytosine and rearranged base pairing.

    abstract::Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine-5)-methyltransferase (DCMtase), M. HaeIII, bound covalently to DNA. In this complex, the substrate cytosine is extruded from the DNA ...


    pub_type: 杂志文章


    authors: Reinisch KM,Chen L,Verdine GL,Lipscomb WN

    更新日期:1995-07-14 00:00:00

  • N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency.

    abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...


    pub_type: 杂志文章


    authors: Wang X,Zhao BS,Roundtree IA,Lu Z,Han D,Ma H,Weng X,Chen K,Shi H,He C

    更新日期:2015-06-04 00:00:00

  • The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations.

    abstract::The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains has been investigated. Seven independent mutations at the white locus were examined, and these fell into two classes on the basis of their genetic and structural properties. The five mutations comprising the f...


    pub_type: 杂志文章


    authors: Rubin GM,Kidwell MG,Bingham PM

    更新日期:1982-07-01 00:00:00