Abstract:
:Transcription hinders replication fork progression and stability. The ATR checkpoint and specialized DNA helicases assist DNA synthesis across transcription units to protect genome integrity. Combining genomic and genetic approaches together with the analysis of replication intermediates, we searched for factors coordinating replication with transcription. We show that the Sen1/Senataxin DNA/RNA helicase associates with forks, promoting their progression across RNA polymerase II (RNAPII)-transcribed genes. sen1 mutants accumulate aberrant DNA structures and DNA-RNA hybrids while forks clash head-on with RNAPII transcription units. These replication defects correlate with hyperrecombination and checkpoint activation in sen1 mutants. The Sen1 function at the forks is separable from its role in RNA processing. Our data, besides unmasking a key role for Senataxin in coordinating replication with transcription, provide a framework for understanding the pathological mechanisms caused by Senataxin deficiencies and leading to the severe neurodegenerative diseases ataxia with oculomotor apraxia type 2 and amyotrophic lateral sclerosis 4.
journal_name
Celljournal_title
Cellauthors
Alzu A,Bermejo R,Begnis M,Lucca C,Piccini D,Carotenuto W,Saponaro M,Brambati A,Cocito A,Foiani M,Liberi Gdoi
10.1016/j.cell.2012.09.041subject
Has Abstractpub_date
2012-11-09 00:00:00pages
835-846issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(12)01244-5journal_volume
151pub_type
杂志文章相关文献
CELL文献大全abstract::The genetic structure of Mason-Pfizer monkey virus (MPMV), a D-type retrovirus, has been determined. In addition to the viral gag, pol, and env genes is an ORF overlapping both gag and pol and that encodes the viral protease. Surprisingly, the MPMV env protein is highly homologous to that of the avian C-type virus, re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90323-5
更新日期:1986-05-09 00:00:00
abstract::Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90401-9
更新日期:1982-11-01 00:00:00
abstract::The in vitro folding pathway of bovine pancreatic trypsin inhibitor (BPTI) has been described previously in terms of the disulfide-bonded intermediates that accumulate during folding of the protein. Folding is slow, occurring in hours at pH 7.3, 25 degrees C. In addition, approximately half of the BPTI molecules becom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90559-u
更新日期:1992-11-27 00:00:00
abstract::I have identified an activity in crude HeLa cell extracts that satisfies the requirements for a human telomere terminal transferase or telomerase. It catalyzes the addition of a 6 nucleotide repeating pattern to oligonucleotide primers containing human or nonhuman telomeric repeat sequences. Direct sequence analyses o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90035-4
更新日期:1989-11-03 00:00:00
abstract::A gene, bcl-3, is found on chromosome 19 adjacent to the breakpoints in the translocation t(14;19)(q32;q13.1), which occurs in some cases of chronic lymphocytic leukemia. Sequence analysis of the human bcl-3 gene predicts a protein containing seven tandem copies of the SWI6/cdc10 motif. This motif was previously ident...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90347-h
更新日期:1990-03-23 00:00:00
abstract::TOR complex 1 (TORC1) is a potent anabolic regulator of cellular growth and metabolism. When cells have sufficient amino acids, TORC1 is active due to its lysosomal localization mediated via the Rag GTPases. Upon amino acid removal, the Rag GTPases release TORC1, causing it to become cytoplasmic and inactive. We show ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.01.024
更新日期:2014-02-13 00:00:00
abstract::Gene silencing is instrumental to interrogate gene function and holds promise for therapeutic applications. Here, we repurpose the endogenous retroviruses' silencing machinery of embryonic stem cells to stably silence three highly expressed genes in somatic cells by epigenetics. This was achieved by transiently expres...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.09.006
更新日期:2016-09-22 00:00:00
abstract::A key aspect of genomic medicine is to make individualized clinical decisions from personal genomes. We developed a machine-learning framework to integrate personal genomes and electronic health record (EHR) data and used this framework to study abdominal aortic aneurysm (AAA), a prevalent irreversible cardiovascular ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.07.021
更新日期:2018-09-06 00:00:00
abstract::Natural killer (NK) cells play a key role in innate immunity by detecting alterations in self and non-self ligands via paired NK cell receptors (NKRs). Despite identification of numerous NKR-ligand interactions, physiological ligands for the prototypical NK1.1 orphan receptor remain elusive. Here, we identify a viral ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.03.002
更新日期:2017-03-23 00:00:00
abstract::The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90095-8
更新日期:1983-12-01 00:00:00
abstract::Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova tel...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81720-1
更新日期:1998-12-23 00:00:00
abstract::Using a cloned cDNA complementary to a portion of the mRNA for the 50 kd human epidermal keratin, we have screened a human genomic library and have isolated and sequenced the gene encoding this keratin. A comparison of the keratin gene with the very distantly related vimentin gene has enabled us to explore the relatio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90456-2
更新日期:1984-12-01 00:00:00
abstract::The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued to preserve post-translational modifications, including protein phosphorylation a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.10.036
更新日期:2020-11-25 00:00:00
abstract::Hsp33, a member of a newly discovered heat shock protein family, was found to be a very potent molecular chaperone. Hsp33 is distinguished from all other known molecular chaperones by its mode of functional regulation. Its activity is redox regulated. Hsp33 is a cytoplasmically localized protein with highly reactive c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80547-4
更新日期:1999-02-05 00:00:00
abstract::We describe the purification and characterization of ACF, an ATP-utilizing chromatin assembly and remodeling factor. ACF is a multisubunit factor that contains ISWI protein and is distinct from NURF, another ISWI-containing factor. In chromatin assembly, purified ACF and a core histone chaperone (such as NAP-1 or CAF-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80321-9
更新日期:1997-07-11 00:00:00
abstract::The recognition of specific DNA-binding sites by transcription factors is a critical yet poorly understood step in the control of gene expression. Members of the Hox family of transcription factors bind DNA by making nearly identical major groove contacts via the recognition helices of their homeodomains. In vivo spec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.09.024
更新日期:2007-11-02 00:00:00
abstract::The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90229-5
更新日期:1983-11-01 00:00:00
abstract::ROAM mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT alpha diploid strains which cannot conjugate than in haplolid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproductio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90353-0
更新日期:1980-11-01 00:00:00
abstract::The double-stranded RNA-activated protein kinase (PKR) was originally identified as a sensor of virus infection, but its function in the brain remains unknown. Here, we report that the lack of PKR enhances learning and memory in several behavioral tasks while increasing network excitability. In addition, loss of PKR i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.029
更新日期:2011-12-09 00:00:00
abstract::Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.11.018
更新日期:2018-01-25 00:00:00
abstract::Nuclear receptors undergo ligand-dependent conformational changes that are required for corepressor-coactivator exchange, but whether there is an actual requirement for specific epigenetic landmarks to impose ligand dependency for gene activation remains unknown. Here we report an unexpected and general strategy that ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.12.038
更新日期:2007-02-09 00:00:00
abstract::How is the characteristic shape of an organelle generated? Recent work has provided insight into how the tubular network of the endoplasmic reticulum (ER) is formed. The tubules themselves are shaped by the reticulons and DP1/Yop1p, whereas their fusion into a network is brought about by membrane-bound GTPases that in...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.11.022
更新日期:2011-12-09 00:00:00
abstract::Human evolution is characterized by a dramatic increase in brain size and complexity. To probe its genetic basis, we examined the evolution of genes involved in diverse aspects of nervous system biology. We found that these genes display significantly higher rates of protein evolution in primates than in rodents. Impo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.040
更新日期:2004-12-29 00:00:00
abstract::Investigating the family tree of a tumor to identify its cellular origins is a daunting task. Liu et al. (2011) now use an elegant lineage tracing technique (MADM) to visualize glioma from its earliest stages. They show that mutations originally induced in neural stem cells lie dormant and only trigger malignant trans...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2011.06.047
更新日期:2011-07-22 00:00:00
abstract::The HIV-1 matrix (MA) protein contains two subcellular localization signals with opposing effects. A myristoylated N-terminus governs particle assembly at the plasma membrane, and a nucleophilic motif facilitates import of the viral preintegration complex into the nucleus of nondividing cells. Here, we show that myris...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90488-3
更新日期:1995-02-10 00:00:00
abstract::Axonal transport has been intensively examined as a good model for studying the mechanism of organelle transport in cells, but it is still unclear how different types of membrane organelles are transported through the nerve axon. To elucidate the function of this mechanism, we have cloned KIF1A, a novel neuron-specifi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90538-3
更新日期:1995-06-02 00:00:00
abstract::Sirtuins are thought to form crucial links between energy levels and cellular metabolism. Libert et al. now provide evidence that SIRT1 activity in the brain modifies mammalian emotional behavior via monoamine signaling and that changes in this pathway might contribute to human affective disorders. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2011.11.052
更新日期:2011-12-23 00:00:00
abstract::Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00986-3
更新日期:2003-12-26 00:00:00
abstract::Enduring forms of synaptic plasticity and memory require new protein synthesis, but little is known about the underlying regulatory mechanisms. Here, we investigate the role of MAPK signaling in these processes. Conditional expression of a dominant-negative form of MEK1 in the postnatal murine forebrain inhibited ERK ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(04)00115-1
更新日期:2004-02-06 00:00:00
abstract::The advent of H7N9 in early 2013 is of concern for a number of reasons, including its capability to infect humans, the lack of clarity in the etiology of infection, and because the human population does not have pre-existing immunity to the H7 subtype. Earlier sequence analyses of H7N9 hemagglutinin (HA) point to amin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.05.034
更新日期:2013-06-20 00:00:00