Abstract：
:Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms must also function during mitosis. We have uncovered a mechanism for regulating transport that is controlled by M phase specific molecular rearrangements in the nuclear pore complex (NPC). These changes allow a transport inhibitory nucleoporin, Nup53p, to bind the karyopherin Kap121p specifically during mitosis, slowing its movement through the NPC and inducing cargo release. Yeast strains that possess defects in the function of Kap121p or the fidelity of the inhibitory pathway are delayed in mitosis. We propose that fluctuations in Kap121p transport mediated by the NPC contribute to controlling the subcellular distribution of molecules that direct progression through mitosis.
journal_name
Celljournal_title
Cellauthors
Makhnevych T,Lusk CP,Anderson AM,Aitchison JD,Wozniak RWdoi
10.1016/s0092-8674(03)00986-3subject
Has Abstractpub_date
2003-12-26 00:00:00pages
813-23issue
7eissn
0092-8674issn
1097-4172pii
S0092867403009863journal_volume
115pub_type
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