Longitudinal Analysis of the Human B Cell Response to Ebola Virus Infection.

abstract：:The genetic dependencies of human cancers widely vary. Here, we catalog this heterogeneity and use it to identify functional gene interactions and genotype-dependent liabilities in cancer. By using genome-wide CRISPR-based screens, we generate a gene essentiality dataset across 14 human acute myeloid leukemia (AML) ce...

journal_title：Cell

authors： Wang T,Yu H,Hughes NW,Liu B,Kendirli A,Klein K,Chen WW,Lander ES,Sabatini DM

更新日期：2017-02-23 00:00:00

abstract：:Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM Na...

journal_title：Cell

authors： Thoma F,Koller T

更新日期：1977-09-01 00:00:00

abstract：:One of the important events in synapse formation is the accumulation of neurotransmitter receptors beneath the presynaptic nerve terminal. Agrin is a component of the synaptic basal lamina that induces the clustering of acetylcholine receptors when bath-applied to muscle fibers in culture. When a cDNA encoding a putat...

journal_title：Cell

authors： Campanelli JT,Hoch W,Rupp F,Kreiner T,Scheller RH

更新日期：1991-11-29 00:00:00

abstract：:Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipop...

journal_title：Cell

authors： Parnas O,Jovanovic M,Eisenhaure TM,Herbst RH,Dixit A,Ye CJ,Przybylski D,Platt RJ,Tirosh I,Sanjana NE,Shalem O,Satija R,Raychowdhury R,Mertins P,Carr SA,Zhang F,Hacohen N,Regev A

更新日期：2015-07-30 00:00:00

abstract：:Type I spiral ganglion neurons (SGNs) transmit sound information from cochlear hair cells to the CNS. Using transcriptome analysis of thousands of single neurons, we demonstrate that murine type I SGNs consist of subclasses that are defined by the expression of subsets of transcription factors, cell adhesion molecules...

journal_title：Cell

authors： Sun S,Babola T,Pregernig G,So KS,Nguyen M,Su SM,Palermo AT,Bergles DE,Burns JC,Müller U

更新日期：2018-08-23 00:00:00

abstract：:Two dimensional gel electrophoresis (triton-acid-urea followed by SDS) has been used to resolve two previously uncharacterized, quantitatively minor histone variants in acid extracts from macronuclei of Tetrahymena thermophila. Utilizing techniques which allow characterization of these variants without purifying them ...

journal_title：Cell

authors： Allis CD,Glover CV,Bowen JK,Gorovsky MA

更新日期：1980-07-01 00:00:00

abstract：:We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...

journal_title：Cell

authors： Woodland HR,Flynn JM,Wyllie AJ

更新日期：1979-09-01 00:00:00

abstract：:Mutations in four nuclear genes, kar1 cdc4, 28, and 37, block or impair nuclear fusion during conjugation of Saccharomyces cerevisiae. Mutations in all four genes are recessive for the caryogamy defect; in matings between diploid cells both of which are heterozygous for any one of the four mutations (-/+ X -/+), caryo...

journal_title：Cell

authors： Dutcher SK,Hartwell LH

更新日期：1983-05-01 00:00:00

abstract：:The hsp 70 gene of Drosophila contains three domains to which a heat shock gene specific transcription factor (HSTF) binds. In addition to the previously described 55 bp binding domain proximal to the TAT homology, there are two 25 bp binding sites farther upstream. Footprinting studies with 5' and 3' deletion mutatio...

journal_title：Cell

authors： Topol J,Ruden DM,Parker CS

更新日期：1985-09-01 00:00:00

abstract：:After translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in posttermination complexes (post-TCs), which must therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subunits. Recycling of bacterial post-TCs requires elongation factor EF-G ...

journal_title：Cell

authors： Pisarev AV,Hellen CU,Pestova TV

更新日期：2007-10-19 00:00:00

abstract：:A basic B-cell-specific protein of 35,000 daltons (XM1) is expressed at a high rate--comparable to actin--in resting splenic B lymphocytes of various species. Splenic or thymic T cells or fibroblasts do not express this protein. Upon polyclonal stimulation of B lymphocytes or in proliferating B-lymphocyte-derived cell...

journal_title：Cell

authors： Rahmsdorf HJ,Mallick U,Ponta H,Herrlich P

更新日期：1982-06-01 00:00:00

abstract：:Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...

journal_title：Cell

authors： Macnab RM,Han DP

更新日期：1983-01-01 00:00:00

abstract：:Mitochondrial DNA from bovine tissue contains heterogeneous sequences located within an evolutionary conserved cytosine homopolymer sequence near the 5' end of the D-loop region. This part of the mammalian mitochondrial genome is known to contain the origin of heavy strand DNA synthesis and the major transcriptional p...

journal_title：Cell

authors： Hauswirth WW,Van de Walle MJ,Laipis PJ,Olivo PD

更新日期：1984-07-01 00:00:00

abstract：:Solute carrier (SLC) membrane transport proteins control essential physiological functions, including nutrient uptake, ion transport, and waste removal. SLCs interact with several important drugs, and a quarter of the more than 400 SLC genes are associated with human diseases. Yet, compared to other gene families of s...

journal_title：Cell

authors： César-Razquin A,Snijder B,Frappier-Brinton T,Isserlin R,Gyimesi G,Bai X,Reithmeier RA,Hepworth D,Hediger MA,Edwards AM,Superti-Furga G

更新日期：2015-07-30 00:00:00

abstract：:Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms ...

journal_title：Cell

authors： Makhnevych T,Lusk CP,Anderson AM,Aitchison JD,Wozniak RW

更新日期：2003-12-26 00:00:00

abstract：:The sites at which topoisomerase I interacts with the transcriptionally active ribosomal chromatin of Xenopus oocytes were mapped by treating oocyte nuclei first with camptothecin to stabilize topoisomerase I-rDNA reaction intermediates, and then with SDS to resolve these adducts as protein-linked nicks in the DNA. St...

journal_title：Cell

authors： Culotta V,Sollner-Webb B

更新日期：1988-02-26 00:00:00

abstract：:Lambda integrative recombination depends on supercoiling of the phage attachment site, attP. Using dimethylsulfate protection and indirect end-labeling, the interaction of the recombination proteins Int and IHF with supercoiled and linear attP has been studied. Supercoiling enhances the binding of Int to attP, but not...

journal_title：Cell

authors： Richet E,Abcarian P,Nash HA

更新日期：1986-09-26 00:00:00

abstract：:The mTOR Complex 1 (mTORC1) pathway regulates cell growth in response to numerous cues, including amino acids, which promote mTORC1 translocation to the lysosomal surface, its site of activation. The heterodimeric RagA/B-RagC/D GTPases, the Ragulator complex that tethers the Rags to the lysosome, and the v-ATPase form...

journal_title：Cell

authors： Bar-Peled L,Schweitzer LD,Zoncu R,Sabatini DM

更新日期：2012-09-14 00:00:00

abstract：:The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...

journal_title：Cell

authors： Arturo EC,Saphire EO

更新日期：2020-10-01 00:00:00

abstract：:ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...

journal_title：Cell

authors： Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

更新日期：2012-10-26 00:00:00

abstract：:During the postpartum period, the brain's inhibitory GABAA receptors may not recover in time following their reduced numbers during pregnancy. This is likely the cause of postpartum depression prevalent in ∼12% of childbearing women. A new therapy for this condition consists of administering a synthetic neurosteroid d...

journal_title：Cell

authors： Mody I

更新日期：2019-01-10 00:00:00

abstract：:Histone DNA of Psammechinus miliaris was obtained in an enriched form by buoyant density gradient centrifugation and was cleaved into 6 kb repeat units (Birnstiel et al., 1975a) by the action of the specific endonucleases EcoRI and HindIII. Since it was suspected that the 6 kb unit harbored all five histone-coding seq...

journal_title：Cell

authors： Schaffner W,Gross K,Telford J,Birnstiel M

更新日期：1976-08-01 00:00:00

abstract：:The organization of neuronal systems is often dependent on activity and competition between cells. In olfaction, the X-linked OCNC1 channel subunit is subject to random inactivation and is essential for odorant-evoked activity. Reporter-tagged OCNC1 mutant mice permit the visualization of OCNC1-deficient olfactory neu...

journal_title：Cell

authors： Zhao H,Reed RR

更新日期：2001-03-09 00:00:00

abstract：:Organ cultures of liver from untreated male Xenopus respond to 17 beta-estradiol in the culture medium by synthesizing and secreting the yolk protein precursor vitellogenin. Vitellogenin synthesis, as a primary response, is first detectable on the fourth day of culture, and comprises up to 12% of the protein synthesiz...

journal_title：Cell

authors： Green CD,Tata JR

更新日期：1976-01-01 00:00:00

abstract：:The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 A resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making exte...

journal_title：Cell

authors： Chai J,Shiozaki E,Srinivasula SM,Wu Q,Datta P,Alnemri ES,Shi Y

更新日期：2001-03-09 00:00:00

abstract：:The phenotype of the autosomal recessive mutation scat includes severe intermittent bleeding, depletion of platelets, and circulating anti-platelet antibodies. In this study, we have mapped the scat mutation to mouse chromosome 8 and shown that the immune component is a secondary consequence of the gene defect. Surpri...

journal_title：Cell

authors： Peters LL,Barker JE

更新日期：1993-07-16 00:00:00

abstract：:The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...

journal_title：Cell

authors： O'Connor CM,Gard DL,Lazarides E

更新日期：1981-01-01 00:00:00

abstract：:A unique DNA structural intermediate associated with termination of replication in Bacillus subtilis has been identified. This was achieved by probing for the Bam HI DNA fragment within which the terminus of the chromosome (terC) resides. The intermediate migrates more slowly than the bulk of digested chromosomal DNA ...

journal_title：Cell

authors： Weiss AS,Wake RG

更新日期：1984-12-01 00:00:00

abstract：:CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that d...

journal_title：Cell

authors： Pawluk A,Amrani N,Zhang Y,Garcia B,Hidalgo-Reyes Y,Lee J,Edraki A,Shah M,Sontheimer EJ,Maxwell KL,Davidson AR

更新日期：2016-12-15 00:00:00

abstract：:The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and...

journal_title：Cell

authors： Waksman G,Shoelson SE,Pant N,Cowburn D,Kuriyan J

更新日期：1993-03-12 00:00:00