Abstract:
:Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM NaCl in 50 mM sodium phosphate buffer (pH 7.0) in the presence of the ion-exchange resin AG 50 W - X2, chromatin appears as "beads-on-a-string" with the nucleosomes separated from each other by distances of about 150-200 A. If chromatin is treated in the presence of the resin with NaCl at concentrations of 650 mM or more, the structural organization of the chromatin is decreased, yielding fibers of irregular appearance.
journal_name
Celljournal_title
Cellauthors
Thoma F,Koller Tdoi
10.1016/0092-8674(77)90188-xsubject
Has Abstractpub_date
1977-09-01 00:00:00pages
101-7issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(77)90188-Xjournal_volume
12pub_type
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