The spliceosome: design principles of a dynamic RNP machine.

abstract：:Short DNA chains, isolated from in vitro pulse-labeled replicating polyoma DNA, exhibit some degree of self-complementarity (28% resistance to S1 nuclease after self-annealing to plateau levels). This level of self-annealing is not increased if short DNA chains present as free single-stranded DNA after extraction are ...

journal_title：Cell

authors： Francke B,Vogt M

更新日期：1975-06-01 00:00:00

abstract：:In an era marked by the increasing prevalence of obesity, diabetes, and cardiovascular disease, the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) has emerged as a transcriptional regulator of metabolism whose activity can be modulated by direct binding of small molecules. As the master ...

journal_title：Cell

authors： Lehrke M,Lazar MA

更新日期：2005-12-16 00:00:00

abstract：:Clinical benefits of cytokine blockade in ileal Crohn's disease (iCD) are limited to a subset of patients. Here, we applied single-cell technologies to iCD lesions to address whether cellular heterogeneity contributes to treatment resistance. We found that a subset of patients expressed a unique cellular module in inf...

journal_title：Cell

authors： Martin JC,Chang C,Boschetti G,Ungaro R,Giri M,Grout JA,Gettler K,Chuang LS,Nayar S,Greenstein AJ,Dubinsky M,Walker L,Leader A,Fine JS,Whitehurst CE,Mbow ML,Kugathasan S,Denson LA,Hyams JS,Friedman JR,Desai PT,Ko

更新日期：2019-09-05 00:00:00

abstract：:Pure primary mesenchymal cells from definitive streak stage chick embryos have been prepared free of epiblast and hypoblast cells. These cells have the potential in culture to differentiate into erythroid cells, beating heart muscle tissue, chondrocytes and epithelial cells. Transformation in vitro of pure primary mes...

journal_title：Cell

authors： Keane RW,Lindblad PC,Pierik LT,Ingram VM

更新日期：1979-08-01 00:00:00

abstract：:A nutritious diet low in calories improves the health and extends the life span of rodents. Recent studies identified a gene, SIR2, which encodes an NAD-dependent deacetylase and may mediate the effects of calorie restriction. In this review, we discuss SIR2 genes and calorie restriction in the lower organisms yeast a...

journal_title：Cell

authors： Guarente L,Picard F

更新日期：2005-02-25 00:00:00

abstract：:The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma. Binding of the initiator to the direct repeats induced a marked bending of the region of gamma replication origin. Binding of the ...

journal_title：Cell

authors： Mukherjee S,Patel I,Bastia D

更新日期：1985-11-01 00:00:00

abstract：:Proteotypes, like genotypes, have been found to vary between individuals in several studies, but consistent molecular functional traits across studies remain to be quantified. In a meta-analysis of 11 proteomics datasets from humans and mice, we use co-variation of proteins in known functional modules across datasets ...

journal_title：Cell

authors： Romanov N,Kuhn M,Aebersold R,Ori A,Beck M,Bork P

更新日期：2019-05-16 00:00:00

abstract：:Telomeres of Oxytricha nova macronuclear chromosomes consist of a repeated T4G4 sequence, single-stranded at the 3' terminus, bound by a heterodimeric protein. The cloning of genes for the two polypeptides and their separate expression in E. coli have enabled evaluation of their individual contributions to DNA binding...

journal_title：Cell

authors： Gray JT,Celander DW,Price CM,Cech TR

更新日期：1991-11-15 00:00:00

abstract：:The 5' terminal sequences of several adenovirus 2 (Ad2) mRNAs, isolated late in infection, are complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed. This has been observed by forming RNA displacement loops (R loops) between Ad2 D...

journal_title：Cell

authors： Chow LT,Gelinas RE,Broker TR,Roberts RJ

更新日期：1977-09-01 00:00:00

abstract：:CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that d...

journal_title：Cell

authors： Pawluk A,Amrani N,Zhang Y,Garcia B,Hidalgo-Reyes Y,Lee J,Edraki A,Shah M,Sontheimer EJ,Maxwell KL,Davidson AR

更新日期：2016-12-15 00:00:00

abstract：:DNA repair is an essential process for preserving genome integrity in all organisms. In eukaryotes, recombinational repair is choreographed by multiprotein complexes that are organized into centers (foci). Here, we analyze the cellular response to DNA double-strand breaks (DSBs) and replication stress in Saccharomyces...

journal_title：Cell

authors： Lisby M,Barlow JH,Burgess RC,Rothstein R

更新日期：2004-09-17 00:00:00

abstract：:In the nucleus of eukaryotic cells, RNA decay in the 3'-5' direction is carried out by a complex of exonucleases called the exosome. Surprisingly, the purified exosome shows only weak activity in vitro. Two papers in this issue of Cell, by LaCava et al. (2005) and Wyers et al. (2005), and a third report by Vanacova et...

journal_title：Cell

authors： Jensen TH,Moore C

更新日期：2005-06-03 00:00:00

abstract：:We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...

journal_title：Cell

authors： Woodland HR,Flynn JM,Wyllie AJ

更新日期：1979-09-01 00:00:00

abstract：:Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...

journal_title：Cell

authors： Selzer G,Som T,Itoh T,Tomizawa J

更新日期：1983-01-01 00:00:00

abstract：:Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...

journal_title：Cell

authors： Zhang X,Yan C,Hang J,Finci LI,Lei J,Shi Y

更新日期：2017-05-18 00:00:00

abstract：:We mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (neor) gene was introduced into an exon of a cloned fragment of the Hprt gene and used to transfect ES cells. Among the G418r col...

journal_title：Cell

authors： Thomas KR,Capecchi MR

更新日期：1987-11-06 00:00:00

abstract：:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains ...

journal_title：Cell

authors： McKnight GL,O'Hara PJ,Parker ML

更新日期：1986-07-04 00:00:00

abstract：:Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...

journal_title：Cell

authors： Beelman CA,Parker R

更新日期：1995-04-21 00:00:00

abstract：:Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscr...

journal_title：Cell

authors： Snyder M,Davis RW

更新日期：1988-09-09 00:00:00

abstract：:The yeast [PSI+] factor is inherited by a prion mechanism involving self-propagating Sup35p aggregates. We find that Sup35p prion function is conserved among distantly related yeasts. As with mammalian prions, a species barrier inhibits prion induction between Sup35p from different yeast species. This barrier is faith...

journal_title：Cell

authors： Santoso A,Chien P,Osherovich LZ,Weissman JS

更新日期：2000-01-21 00:00:00

abstract：:Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...

journal_title：Cell

authors： Brady J,Radonovich M,Vodkin M,Natarajan V,Thoren M,Das G,Janik J,Salzman NP

更新日期：1982-12-01 00:00:00

abstract：:The treatment of advanced prostate cancer has been transformed by novel antiandrogen therapies such as enzalutamide. Here, we identify induction of glucocorticoid receptor (GR) expression as a common feature of drug-resistant tumors in a credentialed preclinical model, a finding also confirmed in patient samples. GR s...

journal_title：Cell

authors： Arora VK,Schenkein E,Murali R,Subudhi SK,Wongvipat J,Balbas MD,Shah N,Cai L,Efstathiou E,Logothetis C,Zheng D,Sawyers CL

更新日期：2013-12-05 00:00:00

abstract：:We have sequenced HFL1 from D. melanogaster, the only cloned hobo element shown to have transposase activity. The 2959 bp HFL1 sequence predicts a 2.0 kb open reading frame (ORF1) with substantial amino acid similarity to the transposases of Activator (Ac) from maize (Zea mays) and Tam3 from snapdragon (Antirrhinum ma...

journal_title：Cell

authors： Calvi BR,Hong TJ,Findley SD,Gelbart WM

更新日期：1991-08-09 00:00:00

abstract：:We have shown that covalent conjugation of ubiquitin to proteins is temperature-sensitive in the mouse cell cycle mutant ts85 due to a specifically thermolabile ubiquitin-activating enzyme (accompanying paper). We show here that degradation of short-lived proteins is also temperature sensitive in ts85 , in contrast to...

journal_title：Cell

authors： Ciechanover A,Finley D,Varshavsky A

更新日期：1984-05-01 00:00:00

abstract：:The molecular basis for membrane association of pp60v-src, the transforming protein of Rous sarcoma virus, was investigated in a cell-free system. Newly synthesized pp60v-src polypeptide, produced by in vitro translation of src mRNA, rapidly bound to plasma membranes. Binding was saturable and dependent on the presenc...

journal_title：Cell

authors： Resh MD

更新日期：1989-07-28 00:00:00

abstract：:The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical prope...

journal_title：Cell

authors： Shimamoto Y,Maeda YT,Ishiwata S,Libchaber AJ,Kapoor TM

更新日期：2011-06-24 00:00:00

abstract：:Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted P...

journal_title：Cell

authors： Woodruff JB,Ferreira Gomes B,Widlund PO,Mahamid J,Honigmann A,Hyman AA

更新日期：2017-06-01 00:00:00

abstract：:As the second dimension to the genome, the epigenome contains key information specific to every type of cells. Thousands of human epigenome maps have been produced in recent years thanks to rapid development of high throughput epigenome mapping technologies. In this review, we discuss the current epigenome mapping too...

journal_title：Cell

authors： Rivera CM,Ren B

更新日期：2013-09-26 00:00:00

abstract：:Mitochondrial Ca(2+) (Ca(2+)(m)) uptake is mediated by an inner membrane Ca(2+) channel called the uniporter. Ca(2+) uptake is driven by the considerable voltage present across the inner membrane (ΔΨ(m)) generated by proton pumping by the respiratory chain. Mitochondrial matrix Ca(2+) concentration is maintained five ...

journal_title：Cell

authors： Mallilankaraman K,Doonan P,Cárdenas C,Chandramoorthy HC,Müller M,Miller R,Hoffman NE,Gandhirajan RK,Molgó J,Birnbaum MJ,Rothberg BS,Mak DO,Foskett JK,Madesh M

更新日期：2012-10-26 00:00:00

abstract：:Membrane subdomains have been implicated in T cell signaling, although their properties and mechanisms of formation remain controversial. Here, we have used single-molecule and scanning confocal imaging to characterize the behavior of GFP-tagged signaling proteins in Jurkat T cells. We show that the coreceptor CD2, th...

journal_title：Cell

authors： Douglass AD,Vale RD

更新日期：2005-06-17 00:00:00