Abstract:
:Members of the myocyte enhancer factor-2 (MEF2) family of MADS domain transcription factors cannot induce myogenesis in transfected fibroblasts, but when coexpressed with the myogenic basic-helix-loop-helix (bHLH) proteins MyoD or myogenin they dramatically increase the extent of myogenic conversion above that seen with either myogenic bHLH factor alone. This cooperativity required direct interactions between the DNA-binding domains of MEF2 and the myogenic bHLH factors, but only one of the factors needed a transactivation domain, and only one of the factors needed to be bound to DNA. These interactions allow either factor to activate transcription through the other's binding site and reveal a novel mechanism for indirect activation of gene expression via protein-protein interactions between the DNA-binding domains of heterologous classes of transcription factors.
journal_name
Celljournal_title
Cellauthors
Molkentin JD,Black BL,Martin JF,Olson ENdoi
10.1016/0092-8674(95)90139-6subject
Has Abstractpub_date
1995-12-29 00:00:00pages
1125-36issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(95)90139-6journal_volume
83pub_type
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