Abstract:
:Cellular compartments that cannot be biochemically isolated are challenging to characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that exist at both excitatory and inhibitory synapses. Normal brain function relies on the careful balance of these opposing neural connections, and understanding how this balance is achieved relies on knowledge of their protein compositions. Using a spatially restricted enzymatic tagging strategy, we mapped the proteomes of two of the most common excitatory and inhibitory synaptic clefts in living neurons. These proteomes reveal dozens of synaptic candidates and assign numerous known synaptic proteins to a specific cleft type. The molecular differentiation of each cleft allowed us to identify Mdga2 as a potential specificity factor influencing Neuroligin-2's recruitment of presynaptic neurotransmitters at inhibitory synapses.
journal_name
Celljournal_title
Cellauthors
Loh KH,Stawski PS,Draycott AS,Udeshi ND,Lehrman EK,Wilton DK,Svinkina T,Deerinck TJ,Ellisman MH,Stevens B,Carr SA,Ting AYdoi
10.1016/j.cell.2016.07.041subject
Has Abstractpub_date
2016-08-25 00:00:00pages
1295-1307.e21issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(16)30991-6journal_volume
166pub_type
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