Transcription of the unrearranged mouse C kappa locus: sequence of the initiation region and comparison of activity with a rearranged V kappa-C kappa gene.


:In cells of the B-lymphocyte lineage, 8.4 kb transcripts are constitutively produced from unrearranged kappa constant region (kappa 0) loci. To help elucidate the molecular basis of this phenomenon, we have determined the nucleotide sequence surrounding the site of transcriptional initiation. The kappa 0 transcripts are initiated within a unique Eco RI fragment located about 8 kb upstream from the C kappa gene. The start site is about 36 nucleotides downstream from a Hogness consensus sequence (TGTAAAT) and nearly 200 nucleotides upstream from a sequence that is similar to those encoding the signal peptides of kappa light chains. These features, which are usually found in the 5' flanking regions of kappa variable region genes, suggest that the kappa 0 initiation sequence may be an evolutionary relic of some common ancestral 5' element. In contrast, there is no discernible V kappa-encoding element in 780 nucleotides of sequence downstream from the initiation site. From pulse-chase-labeling experiments with a pre-B-cell hybridoma line and direct measurements of transcriptional activity in isolated nuclei, we have estimated that the rate of transcription of the kappa 0 locus is significantly lower than that of a rearranged V kappa-C kappa gene. This result, together with the fact that unrearranged V kappa genes are transcriptionally silent, suggests that structural features of both the V kappa and C kappa loci contribute to the overall transcriptional efficiency of a rearranged V kappa-C kappa gene. The 8.4 kb transcripts are not processed into any stable RNA products, despite the fact that they contain some apparently normal splice junctions; rather, they are degraded within the nucleus at about half the rate with which a kappa mRNA precursor is processed. Conceivably, the transcriptional activity of the kappa 0 locus might be a prerequisite for its recombinatorial activity.






Van Ness BG,Weigert M,Coleclough C,Mather EL,Kelley DE,Perry RP




Has Abstract


1981-12-01 00:00:00




3 Pt 2












  • Methotrexate Chemotherapy Induces Persistent Tri-glial Dysregulation that Underlies Chemotherapy-Related Cognitive Impairment.

    abstract::Chemotherapy results in a frequent yet poorly understood syndrome of long-term neurological deficits. Neural precursor cell dysfunction and white matter dysfunction are thought to contribute to this debilitating syndrome. Here, we demonstrate persistent depletion of oligodendrocyte lineage cells in humans who received...


    pub_type: 杂志文章


    authors: Gibson EM,Nagaraja S,Ocampo A,Tam LT,Wood LS,Pallegar PN,Greene JJ,Geraghty AC,Goldstein AK,Ni L,Woo PJ,Barres BA,Liddelow S,Vogel H,Monje M

    更新日期:2019-01-10 00:00:00

  • Going Public in Support of Science.

    abstract::Scientists are stepping up like never before to support science in the public arena. In big and small ways, scientists are adopting creative ideas to promote science. ...


    pub_type: 杂志文章


    authors: Matheson S

    更新日期:2017-04-06 00:00:00

  • A myristoyl/phosphotyrosine switch regulates c-Abl.

    abstract::The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...


    pub_type: 杂志文章


    authors: Hantschel O,Nagar B,Guettler S,Kretzschmar J,Dorey K,Kuriyan J,Superti-Furga G

    更新日期:2003-03-21 00:00:00

  • Rac-GAP alpha-chimerin regulates motor-circuit formation as a key mediator of EphrinB3/EphA4 forward signaling.

    abstract::The ephrin/Eph system plays a central role in neuronal circuit formation; however, its downstream effectors are poorly understood. Here we show that alpha-chimerin Rac GTPase-activating protein mediates ephrinB3/EphA4 forward signaling. We discovered a spontaneous mouse mutation, miffy (mfy), which results in a rabbit...


    pub_type: 杂志文章


    authors: Iwasato T,Katoh H,Nishimaru H,Ishikawa Y,Inoue H,Saito YM,Ando R,Iwama M,Takahashi R,Negishi M,Itohara S

    更新日期:2007-08-24 00:00:00

  • Origins and Proliferative States of Human Oligodendrocyte Precursor Cells.

    abstract::Human cerebral cortex size and complexity has increased greatly during evolution. While increased progenitor diversity and enhanced proliferative potential play important roles in human neurogenesis and gray matter expansion, the mechanisms of human oligodendrogenesis and white matter expansion remain largely unknown....


    pub_type: 杂志文章


    authors: Huang W,Bhaduri A,Velmeshev D,Wang S,Wang L,Rottkamp CA,Alvarez-Buylla A,Rowitch DH,Kriegstein AR

    更新日期:2020-08-06 00:00:00

  • Bcl-xL deamidation is a critical switch in the regulation of the response to DNA damage.

    abstract::The therapeutic value of DNA-damaging antineoplastic agents is dependent upon their ability to induce tumor cell apoptosis while sparing most normal tissues. Here, we show that a component of the apoptotic response to these agents in several different types of tumor cells is the deamidation of two asparagines in the u...


    pub_type: 杂志文章


    authors: Deverman BE,Cook BL,Manson SR,Niederhoff RA,Langer EM,Rosová I,Kulans LA,Fu X,Weinberg JS,Heinecke JW,Roth KA,Weintraub SJ

    更新日期:2002-10-04 00:00:00

  • A washable macromolecule from Fv2rr marrow negatively regulates DNA synthesis in erythropoietic progenitor cells BFU-E.

    abstract::The proportion of BFU-E normally engaged in DNA synthesis is low in adult B6 (C57BL/6) mice of genotype Fv2rr (resistant to Friend erythroleukemia virus), as shown by 3H-thymidine or hydroxyurea "cell suicide" experiments in vivo and vitro. When bone marrow cells from these mice were subjected to a single wash in alph...


    pub_type: 杂志文章


    authors: Axelrad AA,Croizat H,Eskinazi D

    更新日期:1981-10-01 00:00:00

  • LIMP-2 is a receptor for lysosomal mannose-6-phosphate-independent targeting of beta-glucocerebrosidase.

    abstract::beta-glucocerebrosidase, the enzyme defective in Gaucher disease, is targeted to the lysosome independently of the mannose-6-phosphate receptor. Affinity-chromatography experiments revealed that the lysosomal integral membrane protein LIMP-2 is a specific binding partner of beta-glucocerebrosidase. This interaction in...


    pub_type: 杂志文章


    authors: Reczek D,Schwake M,Schröder J,Hughes H,Blanz J,Jin X,Brondyk W,Van Patten S,Edmunds T,Saftig P

    更新日期:2007-11-16 00:00:00

  • From microbes to prions the final proof of the prion hypothesis.

    abstract::Much like the "microbe hypothesis" put forth over 150 years ago, the "prion hypothesis" can be definitely proven only if a prion disease is engendered in a natural host from an infectious prion produced in vitro. In this issue of Cell, come very close to accomplishing this goal by producing a prion disease in a natura...


    pub_type: 评论,杂志文章,评审


    authors: Zou WQ,Gambetti P

    更新日期:2005-04-22 00:00:00

  • A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.

    abstract::Endoplasmic reticulum (ER) membrane contact sites (MCSs) mark positions where endosomes undergo fission for cargo sorting. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to cargo-sorting domains on endosome buds. This strategy identified the ER membrane protein TMCC1, a member of ...


    pub_type: 杂志文章


    authors: Hoyer MJ,Chitwood PJ,Ebmeier CC,Striepen JF,Qi RZ,Old WM,Voeltz GK

    更新日期:2018-09-20 00:00:00

  • Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

    abstract::We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule...


    pub_type: 杂志文章


    authors: Lee JO,Rieu P,Arnaout MA,Liddington R

    更新日期:1995-02-24 00:00:00

  • Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP.

    abstract::RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome...


    pub_type: 杂志文章


    authors: Hafner M,Landthaler M,Burger L,Khorshid M,Hausser J,Berninger P,Rothballer A,Ascano M Jr,Jungkamp AC,Munschauer M,Ulrich A,Wardle GS,Dewell S,Zavolan M,Tuschl T

    更新日期:2010-04-02 00:00:00

  • The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector.

    abstract::We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells wit...


    pub_type: 杂志文章


    authors: Spaete RR,Frenkel N

    更新日期:1982-08-01 00:00:00

  • Phosphorylation of intermediate filament proteins by cAMP-dependent protein kinases.

    abstract::The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...


    pub_type: 杂志文章


    authors: O'Connor CM,Gard DL,Lazarides E

    更新日期:1981-01-01 00:00:00

  • An Autonomous Oscillation Times and Executes Centriole Biogenesis.

    abstract::The accurate timing and execution of organelle biogenesis is crucial for cell physiology. Centriole biogenesis is regulated by Polo-like kinase 4 (Plk4) and initiates in S-phase when a daughter centriole grows from the side of a pre-existing mother. Here, we show that a Plk4 oscillation at the base of the growing cent...


    pub_type: 杂志文章


    authors: Aydogan MG,Steinacker TL,Mofatteh M,Wilmott ZM,Zhou FY,Gartenmann L,Wainman A,Saurya S,Novak ZA,Wong SS,Goriely A,Boemo MA,Raff JW

    更新日期:2020-06-25 00:00:00

  • Heterozygous familial hypercholesterolemia: failure of normal allele to compensate for mutant allele at a regulated genetic locus.

    abstract::In normal human fibroblasts, the synthesis of a cell surface receptor for plasma low density lipoprotein (LDL) is regulated by a sensitive system of feedback suppression. The number of functional LDL receptors declines by more than 20 fold when cellular stores of esterified cholesterol are increased by incubation of c...


    pub_type: 杂志文章


    authors: Goldstein JL,Sobhani MK,Faust JR,Brown MS

    更新日期:1976-10-01 00:00:00

  • Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death.

    abstract::We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...


    pub_type: 杂志文章


    authors: Klein R,Smeyne RJ,Wurst W,Long LK,Auerbach BA,Joyner AL,Barbacid M

    更新日期:1993-10-08 00:00:00

  • Determination of the amount of homology required for recombination in bacteriophage T4.

    abstract::Homology is an important feature of recombination. We have used the rll cistrons of bacteriophage T4 to determine the extent of homology required for recombination. We varied the amount of homologous DNA available for recombination in both marker rescue experiments and deletion-by-deletion crosses. Our results suggest...


    pub_type: 杂志文章


    authors: Singer BS,Gold L,Gauss P,Doherty DH

    更新日期:1982-11-01 00:00:00

  • Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis.

    abstract::RGS proteins are GTPase activators for heterotrimeric G proteins. We report here the 2.8 A resolution crystal structure of the RGS protein RGS4 complexed with G(i alpha1)-Mg2+-GDP-AlF4 . Only the core domain of RGS4 is visible in the crystal. The core domain binds to the three switch regions of G(i alpha1), but does n...


    pub_type: 杂志文章


    authors: Tesmer JJ,Berman DM,Gilman AG,Sprang SR

    更新日期:1997-04-18 00:00:00

  • Maternally transmitted resistance to lymphoma development in mice of reciprocal crosses of the RF/J and AKR/J strains.

    abstract::Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...


    pub_type: 杂志文章


    authors: Mayer A,Struuck FD,Duran-Reynals ML,Lilly F

    更新日期:1980-02-01 00:00:00

  • The three-dimensional structure of HLA-B27 at 2.1 A resolution suggests a general mechanism for tight peptide binding to MHC.

    abstract::Cell surface complexes of class I MHC molecules and bound peptide antigens serve as specific recognition elements controlling the cytotoxic immune response. The 2.1 A structure of the human class I MHC molecule HLA-B27 provides a detailed composite image of a co-crystallized collection of HLA-B27-bound peptides, indic...


    pub_type: 杂志文章


    authors: Madden DR,Gorga JC,Strominger JL,Wiley DC

    更新日期:1992-09-18 00:00:00

  • Identification and purification of a protein that binds the yeast ARS consensus sequence.

    abstract::We have identified a yeast protein that binds specifically to the ARS consensus sequence. By two-step chromatography we have purified the factor to apparent homogeneity as a single polypeptide of 67 kd. The purified ARS consensus-binding protein (ACBP) recognizes the ARS consensus of the four genomic ARS elements test...


    pub_type: 杂志文章


    authors: Hofmann JF,Gasser SM

    更新日期:1991-03-08 00:00:00

  • Back to the future with ubiquitin.

    abstract::Two papers published in 1984 by the Varshavsky laboratory revealed that the ubiquitin/proteasome pathway is the principal system for degradation of short-lived proteins in mammalian cells, setting the stage for future demonstrations of this pathway's many regulatory roles. This perspective discusses the impact of thos...


    pub_type: 评论,杂志文章,评审


    authors: Pickart CM

    更新日期:2004-01-23 00:00:00

  • The pro region of BPTI facilitates folding.

    abstract::The in vitro folding pathway of bovine pancreatic trypsin inhibitor (BPTI) has been described previously in terms of the disulfide-bonded intermediates that accumulate during folding of the protein. Folding is slow, occurring in hours at pH 7.3, 25 degrees C. In addition, approximately half of the BPTI molecules becom...


    pub_type: 杂志文章


    authors: Weissman JS,Kim PS

    更新日期:1992-11-27 00:00:00

  • It takes a (dysfunctional) village to raise a tumor.

    abstract::The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...


    pub_type: 评论,杂志文章


    authors: Reilly KM,Van Dyke T

    更新日期:2008-10-31 00:00:00

  • The LIM domain is a modular protein-binding interface.

    abstract::LIM domains are zinc-binding protein sequences that are found in a growing number of proteins, including certain transcriptional regulators, proto-oncogene products, and adhesion plaque constituents. Here we define the biological activity of the LIM domain through studies of an adhesion plaque protein called zyxin tha...


    pub_type: 杂志文章


    authors: Schmeichel KL,Beckerle MC

    更新日期:1994-10-21 00:00:00

  • The secret life of ACE2 as a receptor for the SARS virus.

    abstract::The membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ACE2) is an essential regulator of heart function. Now, Li at al. identify and characterize an unexpected second function of ACE2 as a partner of the SARS-CoV spike glycoprotein in mediating virus entry and cell fusion. ...


    pub_type: 杂志文章


    authors: Dimitrov DS

    更新日期:2003-12-12 00:00:00

  • Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein.

    abstract::Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed i...


    pub_type: 杂志文章


    authors: Schaffer JE,Lodish HF

    更新日期:1994-11-04 00:00:00

  • DNA sequence organization of the beta-globin complex in the BALB/c mouse.

    abstract::Clones containing five non-adult beta-globin genes were isolated from a library of BALB/c DNA. Together, the newly cloned regions comprise a contiguous block of 32 kb of the mouse genome. Restriction mapping of genomic DNA established the physical linkage of these non-adult genes to the two adult beta-globin genes as ...


    pub_type: 杂志文章


    authors: Jahn CL,Hutchison CA 3rd,Phillips SJ,Weaver S,Haigwood NL,Voliva CF,Edgell MH

    更新日期:1980-08-01 00:00:00

  • Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.

    abstract::Trypanosoma brucei variant surface glycoprotein (VSG) genes are activated either by duplicative (DA) transposition of the gene to a pre-activated expression site or by nonduplicative (NDA) activation of a previously silent telomeric gene. We have obtained a recombinant clone spanning the 5' barren region of the expres...


    pub_type: 杂志文章


    authors: Cully DF,Ip HS,Cross GA

    更新日期:1985-08-01 00:00:00