Abstract:
:Two precursor cell subpopulations have been isolated from the spleen cells of nonimmune mice. The major B cell subpopulation binds high levels of the J11D monoclonal antibody and, upon T cell-dependent antigenic stimulation, gives rise to primary antibody-forming cell clones but not secondary B cells. A minority of the 10%-14% of Ia+ precursors that bind low levels of J11D (J11Dlo) also generate antibody-forming cell clones after primary stimulation. However, over 70% of J11Dlo precursors yield no primary antibody-forming cell clones but instead give rise to secondarily responsive B cells. The existence of a distinct precursor cell subpopulation that is responsible for the generation of B cell memory is further evidenced by the distribution of variable region clonotypes among J11Dlo primary precursors, which resembles the clonotype patterns of secondary B cells, and by the accumulation of somatic mutations in their clonal progeny.
journal_name
Celljournal_title
Cellauthors
Linton PJ L,Decker DJ,Klinman NRdoi
10.1016/0092-8674(89)90761-7subject
Has Abstractpub_date
1989-12-22 00:00:00pages
1049-59issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(89)90761-7journal_volume
59pub_type
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