Abstract：
:The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct interaction between Msl5p and both Mud2p and the U1 snRNP protein Prp40p. This defines a bridge between the two ends of the intron. Msl5p (renamed BBP for branchpoint bridging protein) has a mammalian ortholog, the splicing factor SF1. Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals.
journal_name
Celljournal_title
Cellauthors
Abovich N,Rosbash Mdoi
10.1016/s0092-8674(00)80221-4subject
Has Abstractpub_date
1997-05-02 00:00:00pages
403-12issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80221-4journal_volume
89pub_type
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