Abstract:
:The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-parenchymal cell lineages emerge. Our data detail divergence of vascular and sinusoidal endothelia, including a distinct transcriptional profile for sinusoidal endothelial specification by E8.75. We characterize two distinct mesothelial cell types as well as early hepatic stellate cells and reveal distinct spatiotemporal distributions for these populations. We capture transcriptional profiles for hepatoblast specification and migration, including the emergence of a hepatomesenchymal cell type and evidence for hepatoblast collective cell migration. Further, we identify cell-cell interactions during the organization of the primitive sinusoid. This study provides a comprehensive atlas of liver lineage establishment from the endoderm and mesoderm through to the organization of the primitive sinusoid at single-cell resolution.
journal_name
Celljournal_title
Cellauthors
Lotto J,Drissler S,Cullum R,Wei W,Setty M,Bell EM,Boutet SC,Nowotschin S,Kuo YY,Garg V,Pe'er D,Church DM,Hadjantonakis AK,Hoodless PAdoi
10.1016/j.cell.2020.09.012subject
Has Abstractpub_date
2020-10-29 00:00:00pages
702-716.e14issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(20)31153-3journal_volume
183pub_type
杂志文章相关文献
CELL文献大全abstract::Small nucleolar RNAs represent an abundant, evolutionarily ancient group of noncoding RNAs which possess impressively diverse functions ranging from 2'-O-methylation and pseudouridylation of various classes of RNAs, through nucleolytic processing of rRNAs to the synthesis of telomeric DNA. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)00718-3
更新日期:2002-04-19 00:00:00
abstract::Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.11.017
更新日期:2020-12-23 00:00:00
abstract::IFN-γ-activated macrophages attempt to limit pathogen growth through tryptophan starvation. Zhang et al. demonstrate that Mycobacterium tuberculosis responds to this threat by synthesizing its own tryptophan. Genetic and chemical inhibition of this pathway reduces mycobacterial survival, revealing a new immune-depende...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.11.015
更新日期:2013-12-05 00:00:00
abstract::Mammals can taste a wide repertoire of chemosensory stimuli. Two unrelated families of receptors (T1Rs and T2Rs) mediate responses to sweet, amino acids, and bitter compounds. Here, we demonstrate that knockouts of TRPM5, a taste TRP ion channel, or PLCbeta2, a phospholipase C selectively expressed in taste tissue, ab...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/s0092-8674(03)00071-0
更新日期:2003-02-07 00:00:00
abstract::Mouse embryonic stem (ES) cells grown in serum exhibit greater heterogeneity in morphology and expression of pluripotency factors than ES cells cultured in defined medium with inhibitors of two kinases (Mek and GSK3), a condition known as "2i" postulated to establish a naive ground state. We show that the transcriptom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.026
更新日期:2012-04-27 00:00:00
abstract::The pattern of histone modifications, called the histone code, influences transitions between chromatin states and the regulation of transcriptional activity. Four recent papers describe how plant homeodomain (PHD) finger proteins read part of this code. The PHD finger may promote both gene expression and repression t...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.06.028
更新日期:2006-07-14 00:00:00
abstract::Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.06.014
更新日期:2011-07-22 00:00:00
abstract::Trigger factor (TF) is a molecular chaperone that binds to bacterial ribosomes where it contacts emerging nascent chains, but TF is also abundant free in the cytosol where its activity is less well characterized. In vitro studies show that TF promotes protein refolding. We find here that ribosome-free TF stably associ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.07.044
更新日期:2009-09-04 00:00:00
abstract::Protein function is often regulated by posttranslational modifications (PTMs), and recent advances in mass spectrometry have resulted in an exponential increase in PTM identification. However, the functional significance of the vast majority of these modifications remains unknown. To address this problem, we compiled ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.05.036
更新日期:2012-07-20 00:00:00
abstract::In this issue, Stetson et al. (2008) report a mechanism by which host cells avert an autoimmune response to self-nucleic acids. They show that the nuclease Trex1 prevents the accumulation of DNA derived from endogenous retroelements that, if left unchecked, trigger elevated production of type I interferons leading to ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.08.010
更新日期:2008-08-22 00:00:00
abstract::The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.10.032
更新日期:2010-11-12 00:00:00
abstract::The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80875-2
更新日期:2000-06-09 00:00:00
abstract::A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes de...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90620-9
更新日期:1987-06-19 00:00:00
abstract::The redox-regulated chaperone Hsp33 protects organisms against oxidative stress that leads to protein unfolding. Activation of Hsp33 is triggered by the oxidative unfolding of its own redox-sensor domain, making Hsp33 a member of a recently discovered class of chaperones that require partial unfolding for full chapero...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.01.045
更新日期:2012-03-02 00:00:00
abstract::Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.09.013
更新日期:2011-09-30 00:00:00
abstract::Clathrin-mediated endocytosis is a key mechanism by which cells take up extracellular cargo. In this issue, Shimada et al. (2007) reveal the mode of action of the F-BAR domain, which deepens the initial membrane pit that forms during clathrin-mediated endocytosis. ...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2007.05.006
更新日期:2007-05-18 00:00:00
abstract::Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2018.12.035
更新日期:2019-01-24 00:00:00
abstract::Human cerebral cortex size and complexity has increased greatly during evolution. While increased progenitor diversity and enhanced proliferative potential play important roles in human neurogenesis and gray matter expansion, the mechanisms of human oligodendrogenesis and white matter expansion remain largely unknown....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.06.027
更新日期:2020-08-06 00:00:00
abstract::Cholera toxin is assembled from two subunits in the periplasm of Vibrio cholerae and disassembled in the analogous compartment of target cells, the lumen of the endoplasmic reticulum (ER), before a fragment of it, the A1 chain, is transported into the cytosol. We show that protein disulfide isomerase (PDI) in the ER l...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00289-6
更新日期:2001-03-23 00:00:00
abstract::Mitochondrial Ca(2+) (Ca(2+)(m)) uptake is mediated by an inner membrane Ca(2+) channel called the uniporter. Ca(2+) uptake is driven by the considerable voltage present across the inner membrane (ΔΨ(m)) generated by proton pumping by the respiratory chain. Mitochondrial matrix Ca(2+) concentration is maintained five ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.10.011
更新日期:2012-10-26 00:00:00
abstract::How transcription is silenced in early embryos has long been a mystery. In this issue, Guven-Ozkan et al. (2008) report that transcriptional repression during worm embryogenesis is mediated through sequestration of the general transcription factor TAF-4 and is regulated by mechanisms that orchestrate the transition be...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.09.021
更新日期:2008-10-03 00:00:00
abstract::We have used cloned chicken cDNA sequences for alpha- and beta-tubulin to investigate tubulin gene organization in Drosophila melanogaster. Experiments using genomic Drosophila DNA from several sources indicate that there are at least four copies each of the alpha-tubulin gene and the beta-tubulin gene. In situ hybrid...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90561-9
更新日期:1980-12-01 00:00:00
abstract::It was previously demonstrated that the 60,000 dalton (p60) precursor-like polyprotein containing murine p30 was a constituent of the feline leukemia virus pseudotype of Moloney sarcoma virus [m1MSV(FeLV)]. It is now shown that p60 is detected in cells of five mammalian species transformed by m1MSV, indicating that p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90142-8
更新日期:1977-01-01 00:00:00
abstract::xol-1 is the earliest-acting gene in the known hierarchy that controls C. elegans sex determination and dosage compensation. We show that the primary sex-determining signal (the X/A ratio) directs the choice of sexual fate by regulating xol-1 transcript levels: high xol-1 expression during gastrulation triggers male d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90452-2
更新日期:1995-01-13 00:00:00
abstract::Regulatory T cells (Tregs) play a pivotal role in the inhibition of anti-tumor immune responses. Understanding the mechanisms governing Treg homeostasis may therefore be important for development of effective tumor immunotherapy. We have recently demonstrated a key role for the canonical nuclear factor κB (NF-κB) subu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.08.004
更新日期:2017-09-07 00:00:00
abstract::c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90663-b
更新日期:1993-01-29 00:00:00
abstract::Gram-negative bacteria have an outer membrane (OM) that functions as a barrier to protect the cell from toxic compounds such as antibiotics and detergents. The OM is a highly asymmetric bilayer composed of phospholipids, glycolipids, and proteins. Assembly of this essential organelle occurs outside the cytoplasm in an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.02.015
更新日期:2005-04-22 00:00:00
abstract::During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dyna...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.002
更新日期:2011-11-23 00:00:00
abstract::E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.045
更新日期:2014-05-22 00:00:00
abstract::Nuclear pore complexes (NPCs) mediate transport across the nuclear envelope. In yeast, they also interact with active genes, attracting or retaining them at the nuclear periphery. In higher eukaryotes, some NPC components (nucleoporins) are also found in the nucleoplasm, with a so far unknown function. We have functio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.01.011
更新日期:2010-02-05 00:00:00