Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli.

abstract：:New biological tools provide new techniques to probe fundamental biological processes. Here we describe the burgeoning field of proteolysis-targeting chimeras (PROTACs), which are capable of modulating protein concentrations at a post-translational level by co-opting the ubiquitin-proteasome system. We describe the PR...

journal_title：Cell

authors： Burslem GM,Crews CM

更新日期：2020-04-02 00:00:00

abstract：SUMMARY:Disruption of the cyclin-dependent kinase-inhibitory domain of p27 enhances growth of mice. Growth is attributed to an increase in cell number, due to increased cell proliferation, most obviously in tissues that ordinarily express p27 at the highest levels. Disruption of p27 function leads to nodular hyperplasi...

journal_title：Cell

authors： Kiyokawa H,Kineman RD,Manova-Todorova KO,Soares VC,Hoffman ES,Ono M,Khanam D,Hayday AC,Frohman LA,Koff A

更新日期：1996-05-31 00:00:00

abstract：:Hunger and thirst have distinct goals but control similar ingestive behaviors, and little is known about neural processes that are shared between these behavioral states. We identify glutamatergic neurons in the peri-locus coeruleus (periLCVGLUT2 neurons) as a polysynaptic convergence node from separate energy-sensiti...

journal_title：Cell

authors： Gong R,Xu S,Hermundstad A,Yu Y,Sternson SM

更新日期：2020-09-17 00:00:00

abstract：:The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...

journal_title：Cell

authors： Chistol G,Walter JC

更新日期：2015-04-23 00:00:00

abstract：:On each side of the midline of the Drosophila CNS, axons are organized into a series of parallel pathways. Here we show that the midline repellent Slit, previously identified as a short-range signal that regulates midline crossing, also functions at long range to pattern these longitudinal pathways. In this long-range...

journal_title：Cell

authors： Rajagopalan S,Vivancos V,Nicolas E,Dickson BJ

更新日期：2000-12-22 00:00:00

abstract：:Proteins with dibasic retention motifs are subject to retrograde transport to endoplasmic reticulum (ER) by COPI-coated vesicles. As forward transport requires escape from ER retention, general release mechanisms have been expected. Here, KCNK3 potassium channels are shown to bear two cytoplasmic trafficking motifs: a...

journal_title：Cell

authors： O'Kelly I,Butler MH,Zilberberg N,Goldstein SA

更新日期：2002-11-15 00:00:00

abstract：:Understanding how transcriptional enhancers control over 20,000 protein-coding genes to maintain cell-type-specific gene expression programs in all human cells is a fundamental challenge in regulatory biology. Recent studies suggest that gene regulatory elements and their target genes generally occur within insulated ...

journal_title：Cell

authors： Hnisz D,Day DS,Young RA

更新日期：2016-11-17 00:00:00

abstract：:Recently, more than 1000 large intergenic noncoding RNAs (lincRNAs) have been reported. These RNAs are evolutionarily conserved in mammalian genomes and thus presumably function in diverse biological processes. Here, we report the identification of lincRNAs that are regulated by p53. One of these lincRNAs (lincRNA-p21...

journal_title：Cell

authors： Huarte M,Guttman M,Feldser D,Garber M,Koziol MJ,Kenzelmann-Broz D,Khalil AM,Zuk O,Amit I,Rabani M,Attardi LD,Regev A,Lander ES,Jacks T,Rinn JL

更新日期：2010-08-06 00:00:00

abstract：:A family of novel substrates was designed to enable the efficient accumulation of intermediates in site-specific recombination. Strategically placed nicks allow these "suicide substrates" to initiate the reaction but prevent its completion or reversal. Consequently, it has been possible to determine that lambda site-s...

journal_title：Cell

authors： Nunes-Düby SE,Matsumoto L,Landy A

更新日期：1987-08-28 00:00:00

abstract：:The subcellular localization of the mouse Ltk transmembrane protein tyrosine kinase was studied in transfected COS cells, a mature B lymphocyte line, and a low expressing transfected lymphocyte clone. Indirect immunofluorescence and immunogold staining of COS transfectants and endoglycosidase analysis of both COS tran...

journal_title：Cell

authors： Bauskin AR,Alkalay I,Ben-Neriah Y

更新日期：1991-08-23 00:00:00

abstract：:The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amoun...

journal_title：Cell

authors： Kurilla MG,Keene JD

更新日期：1983-10-01 00:00:00

abstract：:Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites i...

journal_title：Cell

authors： Reed R,Maniatis T

更新日期：1986-08-29 00:00:00

abstract：:The JAK/STAT signaling pathway, renowned for its effects on cell proliferation and survival, is constitutively active in various human cancers, including ovarian. We have found that JAK and STAT are required to convert the border cells in the Drosophila ovary from stationary, epithelial cells to migratory, invasive ce...

journal_title：Cell

authors： Silver DL,Montell DJ

更新日期：2001-12-28 00:00:00

abstract：:Steroid hormones induce transcription from the mouse mammary tumor virus (MMTV) promoter by complex mechanisms requiring binding of the hormone receptors to the hormone responsive element (HRE) of the long terminal repeat region. Here we show that the MMTV promoter contains two degenerated octamer motifs immediately u...

journal_title：Cell

authors： Brüggemeier U,Kalff M,Franke S,Scheidereit C,Beato M

更新日期：1991-02-08 00:00:00

abstract：:In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...

journal_title：Cell

authors： Guo Y,Li D,Zhang S,Yang Y,Liu JJ,Wang X,Liu C,Milkie DE,Moore RP,Tulu US,Kiehart DP,Hu J,Lippincott-Schwartz J,Betzig E,Li D

更新日期：2018-11-15 00:00:00

abstract：:Reprogramming to induced pluripotent stem cells (iPSCs) proceeds in a stepwise manner with reprogramming factor binding, transcription, and chromatin states changing during transitions. Evidence is emerging that epigenetic priming events early in the process may be critical for pluripotency induction later. Chromatin ...

journal_title：Cell

authors： Papp B,Plath K

更新日期：2013-03-14 00:00:00

abstract：:The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by lon...

journal_title：Cell

authors： Campuzano S,Carramolino L,Cabrera CV,Ruíz-Gómez M,Villares R,Boronat A,Modolell J

更新日期：1985-02-01 00:00:00

abstract：:The mRNAs from two yeast mitochondrial genes cob-box (cytochrome b) and oxi-3 (cytochrome oxidase 40,000 dalton subunit) are processed from large (7-10 kb) precursors. Certain mutations in each gene block the maturation of the RNAs from both genes at a variety of specific steps. The pleiotropic cytochrome b mutants se...

journal_title：Cell

authors： Church GM,Slonimski PP,Gilbert W

更新日期：1979-12-01 00:00:00

abstract：:Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed i...

journal_title：Cell

authors： Schaffer JE,Lodish HF

更新日期：1994-11-04 00:00:00

abstract：:The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...

journal_title：Cell

authors： Zhang YZ,Holmes EC

更新日期：2020-04-16 00:00:00

abstract：:Cell lines from rhabdomyosarcomas, which are tumors of muscle origin, have been used as models of CD4-independent HIV infection. These cell lines can be induced to differentiate in vitro. We report here that the vpr gene of HIV1 is sufficient for the differentiation of the human rhabdomyosarcoma cell line TE671. Diffe...

journal_title：Cell

authors： Levy DN,Fernandes LS,Williams WV,Weiner DB

更新日期：1993-02-26 00:00:00

abstract：:I have defined conditions under which RepFIC plasmid DNA can be maintained in a state of lowered helical density. In exponentially growing cultures, the DNA of lowered helical density is present in small amounts but never totally absent, suggesting that it is a normal variant of plasmid maintenance. It is fully methyl...

journal_title：Cell

authors： Maas R

更新日期：2001-06-29 00:00:00

abstract：:Prion-like domains have emerged as important drivers of neurodegenerative disease. Now, Boulay et al. establish that the translocated prion-like domain of the oncogenic EWS-FLI1 fusion protein enables phase-separation events, which inappropriately recruit chromatin-remodeling factors to elicit the aberrant transcripti...

journal_title：Cell

authors： Shorter J

更新日期：2017-09-21 00:00:00

abstract：:TET-mediated 5-methyl cytosine (5mC) oxidation acts in epigenetic regulation, stem cell development, and cancer. Hu et al. now determine the crystal structure of the TET2 catalytic domain bound to DNA, shedding light on 5mC-DNA substrate recognition and the catalytic mechanism of 5mC oxidation. ...

journal_title：Cell

authors： Tsai CL,Tainer JA

更新日期：2013-12-19 00:00:00

abstract：:Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...

journal_title：Cell

authors： Eyler CE,Wu Q,Yan K,MacSwords JM,Chandler-Militello D,Misuraca KL,Lathia JD,Forrester MT,Lee J,Stamler JS,Goldman SA,Bredel M,McLendon RE,Sloan AE,Hjelmeland AB,Rich JN

更新日期：2011-07-08 00:00:00

abstract：:The differentiation of body-wall muscle cells was studied in the nematode Caenorhabditis elegans. Specific antibodies to myosin and paramyosin, major protein constituents of differentiated muscle, react with mesodermal cells in wild-type embryos towards the end of the first half of embryogenesis. Immunoreactive cells ...

journal_title：Cell

authors： Gossett LA,Hecht RM,Epstein HF

更新日期：1982-08-01 00:00:00

abstract：:We have compared the chromatin structure in the active and inactive states at loci encoding the major heat shock protein in Drosophila. DNAase I and micrococcal nuclease were used as probes of higher order organization and nucleosomal integrity. Such integrity is gauged here by the characteristic pattern of discrete D...

journal_title：Cell

authors： Wu C,Wong YC,Elgin SC

更新日期：1979-04-01 00:00:00

abstract：:Meiotic recombination occurs preferentially between homologous nonsister chromatids rather than between sisters, opposite to the bias of mitotic recombinational repair. We have examined formation of joint molecule recombination intermediates (JMs) between homologs and between sisters in yeast strains lacking the meiot...

journal_title：Cell

authors： Schwacha A,Kleckner N

更新日期：1997-09-19 00:00:00

abstract：:Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesion...

journal_title：Cell

authors： Lee S,Elenbaas B,Levine A,Griffith J

更新日期：1995-06-30 00:00:00

abstract：:Francis' office window (at the Salk) commanded a panorama of the Pacific. "This grand natural scene was a physical correlate of Francis's intellectual world: wide-ranging, brilliantly lit, a little overawing, but also immensely inviting and above all an exciting place to be." (Mitchison, 2004). ...

journal_title：Cell

authors： Lawrence PA

更新日期：2016-12-01 00:00:00