Abstract:
:Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesions while single-stranded binding proteins did not bind. Gel retardation assays showed that p53 formed highly stable complexes when the DNA contained the IDL mismatches, but only unstable complexes when the DNA lacked lesions (but did contain free ends). The highly stable complexes had a half-life of > 2 hr, suggesting that upon encountering lesions, p53 may recruit other proteins to the site, providing a signal for DNA damage.
journal_name
Celljournal_title
Cellauthors
Lee S,Elenbaas B,Levine A,Griffith Jdoi
10.1016/s0092-8674(05)80006-6subject
Has Abstractpub_date
1995-06-30 00:00:00pages
1013-20issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(05)80006-6journal_volume
81pub_type
杂志文章相关文献
CELL文献大全abstract::Treating B16 mouse melanoma cells with monoclonal antibody NORM-2 reduces cell growth in tissue culture, agar, and syngeneic mice. We show that the NORM-2 antibody recognizes an integral 83 kd glycoprotein that is mobile in the plane of the plasma membrane of B16 melanoma cells. Expression of the glycoprotein is reduc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90510-6
更新日期:1986-12-05 00:00:00
abstract::Five years into the human postgenomic era, we are gaining considerable knowledge about host-pathogen interactions through host genomes. This "infectogenomics" approach should yield further insights into both diagnostic and therapeutic advances, as well as normal cellular function. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.02.003
更新日期:2006-02-24 00:00:00
abstract::How is the characteristic shape of an organelle generated? Recent work has provided insight into how the tubular network of the endoplasmic reticulum (ER) is formed. The tubules themselves are shaped by the reticulons and DP1/Yop1p, whereas their fusion into a network is brought about by membrane-bound GTPases that in...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.11.022
更新日期:2011-12-09 00:00:00
abstract::Type III secretion systems mediate export of virulence proteins and flagellar assembly subunits in Gram-negative bacteria. Chaperones specific to each class of secreted protein are believed to prevent degradation of the secreted substrates. We show that an additional role of chaperones may be to regulate translation o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00053-2
更新日期:2000-08-18 00:00:00
abstract::c-Abl is a tyrosine kinase localized primarily in the nucleus. Previous assays for abl function rely on cellular transformation by abl mutants, which are cytoplasmic. Using a conditional overexpression strategy, we have developed a functional assay for c-abl. Overexpression of c-abl inhibits growth by causing cell cyc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90240-2
更新日期:1994-04-08 00:00:00
abstract::Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.051
更新日期:2015-07-02 00:00:00
abstract::The fluid-mosaic model posits a liquid-like plasma membrane, which can flow in response to tension gradients. It is widely assumed that membrane flow transmits local changes in membrane tension across the cell in milliseconds, mediating long-range signaling. Here, we show that propagation of membrane tension occurs qu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.054
更新日期:2018-12-13 00:00:00
abstract::The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may have an active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptor-like functio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00191-x
更新日期:2001-01-12 00:00:00
abstract::A nutritious diet low in calories improves the health and extends the life span of rodents. Recent studies identified a gene, SIR2, which encodes an NAD-dependent deacetylase and may mediate the effects of calorie restriction. In this review, we discuss SIR2 genes and calorie restriction in the lower organisms yeast a...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.01.029
更新日期:2005-02-25 00:00:00
abstract::Inconsistent with the view that hair follicle stem cells reside in the matrix area of the hair bulb, we found that label-retaining cells exist exclusively in the bulge area of the mouse hair follicle. The bulge consists of a subpopulation of outer root sheath cells located in the midportion of the follicle at the arre...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90696-c
更新日期:1990-06-29 00:00:00
abstract::In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.06.010
更新日期:2010-07-09 00:00:00
abstract::An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90379-3
更新日期:1983-09-01 00:00:00
abstract::X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90379-9
更新日期:1984-06-01 00:00:00
abstract::Induction of pluripotency in somatic cells has been achieved by myriad combinations of transcription factors that belong to the core pluripotency circuitry. In this issue, Shu et al. report reprogramming with lineage specifiers, lending support to the view of the pluripotent state as a fine balance between competing d...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.04.051
更新日期:2013-05-23 00:00:00
abstract::The LIM domain protein rbtn2 is associated with T cell acute leukemias. We demonstrate that rbtn2 is a nuclear protein expressed in the erythroid lineage in vivo, and using homologous recombination, we show that it is essential for erythroid development in mice. The homozygous rbtn2 null mutation leads to failure of y...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90571-1
更新日期:1994-07-15 00:00:00
abstract::Electrophoretic and autoradiographic analyses of the incorporation of 35S--methionine into newly synthesized proteins during myogenesis reveal that presumptive chicken myoblasts synthesize primarily one intermediate filament protein: vimentin. Desmin synthesis is initiated at the onset of fusion. Synthesis rates of bo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90408-0
更新日期:1980-01-01 00:00:00
abstract::During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90076-b
更新日期:1991-11-15 00:00:00
abstract::22q11.2 microdeletions result in specific cognitive deficits and schizophrenia. Analysis of Df(16)A(+/-) mice, which model this microdeletion, revealed abnormalities in the formation of neuronal dendrites and spines, as well as altered brain microRNAs. Here, we show a drastic reduction of miR-185, which resides within...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.11.052
更新日期:2013-01-17 00:00:00
abstract::247 independent events involving insertion of the TN3 transposable element into a 4 kb constructed plasmid (pTU4) of partially known DNA sequence were studied by restriction endonuclease mapping, and 65 of these insertion sites were examined further by DNA sequence analysis. Our results show that the previously propos...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90396-7
更新日期:1980-01-01 00:00:00
abstract::Basal cells are by definition located on the basolateral side of several epithelia, and they have never been observed reaching the lumen. Using high-resolution 3D confocal imaging, we report that basal cells extend long and slender cytoplasmic projections that not only reach toward the lumen but can cross the tight ju...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.10.020
更新日期:2008-12-12 00:00:00
abstract::The genetic structure of Mason-Pfizer monkey virus (MPMV), a D-type retrovirus, has been determined. In addition to the viral gag, pol, and env genes is an ORF overlapping both gag and pol and that encodes the viral protease. Surprisingly, the MPMV env protein is highly homologous to that of the avian C-type virus, re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90323-5
更新日期:1986-05-09 00:00:00
abstract::Alternative splicing (AS) patterns have diverged rapidly during vertebrate evolution, yet the functions of most species- and lineage-specific splicing events are not known. We observe that mammalian-specific AS events are enriched in transcript sequences encoding intrinsically disordered regions (IDRs) of proteins, in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.06.037
更新日期:2017-07-13 00:00:00
abstract::Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.12.044
更新日期:2006-03-24 00:00:00
abstract::Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90016-4
更新日期:1986-03-28 00:00:00
abstract::Oncogenic activation of RAS genes via point mutations occurs in 20%-30% of human cancers. The development of effective RAS inhibitors has been challenging, necessitating new approaches to inhibit this oncogenic protein. Functional studies have shown that the switch region of RAS interacts with a large number of effect...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.03.045
更新日期:2016-04-21 00:00:00
abstract::This year, the Albert Lasker Basic Medical Research Award will be shared by Michael Sheetz, James Spudich, and Ronald Vale for discoveries concerning the biophysical actions of cytoskeletal motor-protein machines that move cargo within cells, contract muscles, and enable cell motility. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.08.031
更新日期:2012-09-14 00:00:00
abstract::The transition from the juvenile to the adult phase of shoot development in plants is accompanied by changes in vegetative morphology and an increase in reproductive potential. Here, we describe the regulatory mechanism of this transition. We show that miR156 is necessary and sufficient for the expression of the juven...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.06.031
更新日期:2009-08-21 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) binds to cells through interactions of viral glycoproteins gB and gC with heparan sulfate chains on cell surface proteoglycans. This binding is not sufficient for viral entry, which requires fusion between the viral envelope and cell membrane. Here, we show that heparan sulfate modi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80058-6
更新日期:1999-10-01 00:00:00
abstract::Epidermal lineage commitment occurs when multipotent stem cells are specified to three lineages: the epidermis, the hair follicle, and the sebaceous gland (SG). How and when a lineage becomes specified remains unknown. Here, we report the existence of a population of unipotent progenitor cells that reside in the SG an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.06.048
更新日期:2006-08-11 00:00:00
abstract::The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90229-5
更新日期:1983-11-01 00:00:00