Abstract:
:c-Abl is a tyrosine kinase localized primarily in the nucleus. Previous assays for abl function rely on cellular transformation by abl mutants, which are cytoplasmic. Using a conditional overexpression strategy, we have developed a functional assay for c-abl. Overexpression of c-abl inhibits growth by causing cell cycle arrest. Growth suppression requires tyrosine kinase activity, nuclear localization, and an intact SH2 domain. Overexpression of dominant negative c-abl disrupts cell cycle control and enhances transformation by tyrosine kinases, G proteins, and transcription factor oncogenes. These findings suggest that c-abl acts as a negative regulator of cell growth. This growth suppressive activity is functionally similar to that of tumor suppressor genes such as p53 and Rb.
journal_name
Celljournal_title
Cellauthors
Sawyers CL,McLaughlin J,Goga A,Havlik M,Witte Odoi
10.1016/0092-8674(94)90240-2subject
Has Abstractpub_date
1994-04-08 00:00:00pages
121-31issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(94)90240-2journal_volume
77pub_type
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