Abstract：
:We have constructed an actin-HIS4 gene fusion, such that expression of HIS4 requires proper splicing of the actin intron. Using this chimeric gene in an in vivo screen for splicing mutations, we have isolated a G to A transition in the fifth position of the yeast 5' consensus sequence/GTAPyGT. This mutation still allows the junction to be recognized by the splicing machinery, albeit inefficiently. Surprisingly, the fidelity of the 5' endonucleolytic cleavage is also reduced. This results in an incorrect cleavage 6 nucleotides 5' of the 5' junction, at the dinucleotide/AT. Cleavage at this abnormal site does not lead to the production of mature mRNA, although this species appears to be in a lariat structure. The behavior of this mutant argues that recognition of the 5' junction and subsequent cleavage are separable events and, furthermore, that requirements for 3' endonucleolytic cleavage may be more complex than previously imagined.
journal_name
Celljournal_title
Cellauthors
Parker R,Guthrie Cdoi
10.1016/0092-8674(85)90065-0subject
Has Abstractpub_date
1985-05-01 00:00:00pages
107-18issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(85)90065-0journal_volume
41pub_type
杂志文章相关文献
CELL文献大全abstract：:The complete sequence of a hemagglutinin (HA) gene of a recent human influenza A strain, A/Victoria/3/75, is 1768 nucleotides long and contains the information for 567 amino acids. It codes for a signal peptide of 16 amino acids, the HA1 chain of the mature hemagglutinin of 329 amino acids, a connecting region between...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(80)80045-6
更新日期：1980-03-01 00:00:00
abstract：:Among its multiple functions, p53 is a critical regulator of TGF-beta responses. Sasai et al. (2008) now identify a new p53 inhibitory protein, XFDL156. During embryonic development, this factor is expressed in the ectoderm germ layer and maintains the pluripotency of ectodermal cells by inhibiting TGF-beta target gen...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.05.013
更新日期：2008-05-30 00:00:00
abstract：:Embryonal carcinoma cell lines present strong developmental analogies with early embryonic cells. Previous studies have shown that treatment of F9 teratocarcinoma cells with retinoic acid induces the expression of the classical transplantation antigens which are indispensable for effective interactions between cells. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90179-4
更新日期：1983-12-01 00:00:00
abstract：:Organ cultures of liver from untreated male Xenopus respond to 17 beta-estradiol in the culture medium by synthesizing and secreting the yolk protein precursor vitellogenin. Vitellogenin synthesis, as a primary response, is first detectable on the fourth day of culture, and comprises up to 12% of the protein synthesiz...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90263-4
更新日期：1976-01-01 00:00:00
abstract：:Mutations in the C. elegans gene ced-3 prevent almost all programmed cell deaths, so that in a ced-3 mutant there are many extra cells. We show that the pharyngeal neuron M4 is essential for feeding in wild-type worms, but in a ced-3 mutant, one of the extra cells, probably MSpaaaaap (the sister of M4), can sometimes ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90593-9
更新日期：1987-12-24 00:00:00
abstract：:Tissue homeostasis requires maintenance of functional integrity under stress. A central source of stress is mechanical force that acts on cells, their nuclei, and chromatin, but how the genome is protected against mechanical stress is unclear. We show that mechanical stretch deforms the nucleus, which cells initially ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.03.052
更新日期：2020-05-14 00:00:00
abstract：:The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. We have solved the X-ray crystal structure to 1.9 A resolution of an autoinhibited, unphosphorylated form of EphB2 comprised of the juxtamembrane region and the kinase domain. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00496-2
更新日期：2001-09-21 00:00:00
abstract：:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90450-1
更新日期：1984-12-01 00:00:00
abstract：:The Drosophila transforming growth factor beta (TGF-beta) homolog Decapentaplegic (Dpp) acts as a morphogen that forms a long-range concentration gradient to direct the anteroposterior patterning of the wing. Both planar transcytosis initiated by Dynamin-mediated endocytosis and extracellular diffusion have been propo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.09.031
更新日期：2004-10-15 00:00:00
abstract：:Junctions that connect the endoplasmic reticulum (ER) and the plasma membrane (PM) are unique yet ubiquitous subcellular compartments. Giordano et al. now report that extended synaptotagmins (E-Syts) promote their formation, providing fundamental insight into the molecular machinery controlling ER and plasma membrane ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2013.05.054
更新日期：2013-06-20 00:00:00
abstract：:We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90098-j
更新日期：1991-05-17 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic itch and inflammatory disorder of the skin that affects one in ten people. Patients suffering from severe AD eventually progress to develop asthma and allergic rhinitis, in a process known as the "atopic march." Signaling between epithelial cells and innate immune cells via the cyto...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.08.057
更新日期：2013-10-10 00:00:00
abstract：:Inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2 are upregulated in many cancer cells. It has been thought that small-molecule mimetics of Smac, an endogenous IAP antagonist, might potentiate apoptosis in cancer cells by promoting caspase activation. However, three recent papers, two in Cell (Vinc...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2007.10.042
更新日期：2007-11-16 00:00:00
abstract：:M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90099-4
更新日期：1981-06-01 00:00:00
abstract：:The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast S...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81391-4
更新日期：1996-11-15 00:00:00
abstract：:We have analyzed the structure of the Shrunken (Sh) locus in a strain containing an unstable recessive mutation, sh-m5933, caused by the transposable controlling element Dissociation (Ds). We have also analyzed nine spontaneous Sh revertant alleles. The sh-m5933 allele contains a 30 kb insertion at the Sh locus, as we...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90372-0
更新日期：1983-09-01 00:00:00
abstract：:CFTR, the protein associated with cystic fibrosis, is phosphorylated on serine residues in response to cAMP agonists. Serines 660, 737, 795, and 813 were identified as in vivo targets for phosphorylation by protein kinase A. The SPQ fluorescence assay revealed that mutagenesis of any one of these sites did not affect ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90446-6
更新日期：1991-09-06 00:00:00
abstract：:Glucose homeostasis is strictly controlled in all domains of life. Bacteria that are unable to balance intracellular sugar levels and deal with potentially toxic phosphosugars cease growth and risk being outcompeted. Here, we identify the conserved haloacid dehalogenase (HAD)-like enzyme YigL as the previously hypothe...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.03.003
更新日期：2013-04-11 00:00:00
abstract：:The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.10.009
更新日期：2008-10-31 00:00:00
abstract：:The functions of caveolae, the characteristic plasma membrane invaginations, remain debated. Their abundance in cells experiencing mechanical stress led us to investigate their role in membrane-mediated mechanical response. Acute mechanical stress induced by osmotic swelling or by uniaxial stretching results in a rapi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.12.031
更新日期：2011-02-04 00:00:00
abstract：:mRNA made in eukaryotic cells typically has a 3' poly(A) tail that is added posttranscriptionally. To investigate mechanisms by which 3' poly(A) is formed, we identified the genes for the two vaccina virus-encoded polypeptides, VP55 and VP39. Primer-dependent polyadenylation activity was associated exclusively with pu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90048-4
更新日期：1991-09-20 00:00:00
abstract：:247 independent events involving insertion of the TN3 transposable element into a 4 kb constructed plasmid (pTU4) of partially known DNA sequence were studied by restriction endonuclease mapping, and 65 of these insertion sites were examined further by DNA sequence analysis. Our results show that the previously propos...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90396-7
更新日期：1980-01-01 00:00:00
abstract：:Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90073-2
更新日期：1975-09-01 00:00:00
abstract：:Calmodulin is implicated as the primary transducer of the calcium signal in pancreatic beta cells, where it is present at very high concentrations. We have produced three lines of transgenic mice carrying a calmodulin minigene regulated by the rat insulin II promoter. Immunohistochemistry and hybridization analyses in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90505-9
更新日期：1989-09-22 00:00:00
abstract：:We have used cloned chicken cDNA sequences for alpha- and beta-tubulin to investigate tubulin gene organization in Drosophila melanogaster. Experiments using genomic Drosophila DNA from several sources indicate that there are at least four copies each of the alpha-tubulin gene and the beta-tubulin gene. In situ hybrid...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90561-9
更新日期：1980-12-01 00:00:00
abstract：:Short DNA chains, isolated from in vitro pulse-labeled replicating polyoma DNA, exhibit some degree of self-complementarity (28% resistance to S1 nuclease after self-annealing to plateau levels). This level of self-annealing is not increased if short DNA chains present as free single-stranded DNA after extraction are ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90028-8
更新日期：1975-06-01 00:00:00
abstract：:In target tissues for estrogen, including breast cancer cells, the synthesis of progesterone receptors (PRs) is controlled by estradiol acting through estrogen receptors (ERs). We describe studies with T47D human breast cancer cells, whose PRs are not regulated by estradiol, though present in extraordinary amounts (30...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90218-5
更新日期：1982-03-01 00:00:00
abstract：:Inflammation is associated with many neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. In this Review, we discuss inducers, sensors, transducers, and effectors of neuroinflammation that contribute to neuronal dysfunction and death. Al...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2010.02.016
更新日期：2010-03-19 00:00:00
abstract：:We report the identification and characterization of a class of IS10 transposase mutants that carry out only some of the steps required for transposition. These mutants were identified among transposition-defective mutants as a specific subclass that retains the wild-type ability to induce SOS functions in the presenc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90299-7
更新日期：1989-10-20 00:00:00
abstract：:In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.09.057
更新日期：2018-11-15 00:00:00
