Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation.

Abstract:

:The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast Sec61p complexes in detergent form cylindrical oligomers with a diameter of approximately 85 A and a central pore of approximately 20 A. Each oligomer contains 3-4 heterotrimers. Similar ring structures are seen in reconstituted proteoliposomes and native membranes. Oligomer formation by the reconstituted Sec61p complex is stimulated by its association with ribosomes or the Sec62/63p complex. We propose that these cylindrical oligomers represent protein-conducting channels of the ER, formed by ligands specific for co- and posttranslational transport.

journal_name

Cell

journal_title

Cell

authors

Hanein D,Matlack KE,Jungnickel B,Plath K,Kalies KU,Miller KR,Rapoport TA,Akey CW

doi

10.1016/s0092-8674(00)81391-4

subject

Has Abstract

pub_date

1996-11-15 00:00:00

pages

721-32

issue

4

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(00)81391-4

journal_volume

87

pub_type

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