Abstract:
:The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast Sec61p complexes in detergent form cylindrical oligomers with a diameter of approximately 85 A and a central pore of approximately 20 A. Each oligomer contains 3-4 heterotrimers. Similar ring structures are seen in reconstituted proteoliposomes and native membranes. Oligomer formation by the reconstituted Sec61p complex is stimulated by its association with ribosomes or the Sec62/63p complex. We propose that these cylindrical oligomers represent protein-conducting channels of the ER, formed by ligands specific for co- and posttranslational transport.
journal_name
Celljournal_title
Cellauthors
Hanein D,Matlack KE,Jungnickel B,Plath K,Kalies KU,Miller KR,Rapoport TA,Akey CWdoi
10.1016/s0092-8674(00)81391-4subject
Has Abstractpub_date
1996-11-15 00:00:00pages
721-32issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81391-4journal_volume
87pub_type
杂志文章相关文献
CELL文献大全abstract::The peripheral nervous system has remarkable regenerative capacities in that it can repair a fully cut nerve. This requires Schwann cells to migrate collectively to guide regrowing axons across a 'bridge' of new tissue, which forms to reconnect a severed nerve. Here we show that blood vessels direct the migrating cord...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.07.021
更新日期:2015-08-27 00:00:00
abstract::We here attempt to achieve an integrated understanding of the structure and dynamics of a number of higher-order assemblies, including amyloids, various kinds of signalosomes, and cellular granules. We propose that the synergy between folded domains, linear motifs, and intrinsically disordered regions regulates the fo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.05.004
更新日期:2016-05-19 00:00:00
abstract::Brefeldin A (BFA) is a fungal metabolite that causes a redistribution of the stacked cisternae of the Golgi complex into the endoplasmic reticulum by inhibiting anterograde transport. We report that BFA also causes membrane tubules derived from the trans-Golgi network (TGN) to fuse with early endosomes. In the presenc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90533-5
更新日期:1991-11-01 00:00:00
abstract::Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.050
更新日期:2012-01-20 00:00:00
abstract::The neurofibromatosis type 1 (NF1) protein contains a region of significant sequence similarity to ras p21 GTPase-activating protein (GAP) and the yeast IRA1 gene product. A fragment of NF1 cDNA encoding the GAP-related domain (NF1 GRD) was expressed, immunoaffinity purified, and assayed for effects on N-ras p21 GTPas...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90150-d
更新日期:1990-11-16 00:00:00
abstract::Two independent approaches to understanding the molecular mechanism of cytokinesis have converged on the gene spaghetti-squash (sqh). A genetic screen for mitotic mutants identified sqh1, a mutation that disrupts cytokinesis, which was then cloned by transposon tagging. Independently, the gene that encodes the regulat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90013-o
更新日期:1991-06-28 00:00:00
abstract::Microbes that cause persistent infections (e.g., herpes viruses) do so by switching from fast-growing lytic states to slow-growing latent states. Waldman et al. have identified a single transcription factor that governs the switch between the lytic and latent forms of Toxoplasma gondii, a parasite that causes a persis...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.12.038
更新日期:2020-01-23 00:00:00
abstract::Signaling proteins from the same family can have markedly different roles in a given cellular context. Here, we show that expression of one hundred constitutively active human small GTPases induced cell morphologies that fell into nine distinct classes. We developed an algorithm for pairs of classes that predicted ami...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00315-5
更新日期:2003-05-02 00:00:00
abstract::Fast-spiking interneurons (FSIs) are a prominent class of forebrain GABAergic cells implicated in two seemingly independent network functions: gain control and network plasticity. Little is known, however, about how these roles interact. Here, we use a combination of cell-type-specific ablation, optogenetics, electrop...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.01.005
更新日期:2018-02-08 00:00:00
abstract::In extracts from rat and calf uterus, the steroid hormone 17 beta-estradiol stimulates the binding of its specific receptor protein to DNA. This interaction appears to be of low affinity (half of the estradiol-activated, 5S receptor bound at 300-400 mug/ml DNA) and nonspecific with respect to DNA base sequence. No bin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90150-6
更新日期:1975-04-01 00:00:00
abstract::The advent of H7N9 in early 2013 is of concern for a number of reasons, including its capability to infect humans, the lack of clarity in the etiology of infection, and because the human population does not have pre-existing immunity to the H7 subtype. Earlier sequence analyses of H7N9 hemagglutinin (HA) point to amin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.05.034
更新日期:2013-06-20 00:00:00
abstract::In budding yeast, septins are involved in the morphogenesis checkpoint and the DNA damage checkpoint, both of which regulate cell-cycle progression. In this issue of Cell, Kremer et al. (2007) link septins to DNA damage in mammalian cells by identifying a new signaling pathway that includes the adaptors SOCS7 and NCK....
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.08.022
更新日期:2007-09-07 00:00:00
abstract::The Clock mutation lengthens periodicity and reduces amplitude of circadian rhythms in mice. The effects of Clock are cell intrinsic and can be observed at the level of single neurons in the suprachiasmatic nucleus. To address how cells of contrasting genotype functionally interact in vivo to control circadian behavio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00294-x
更新日期:2001-04-06 00:00:00
abstract::Morphogen gradients control body pattern by differentially regulating cellular behavior. Here, we analyze the molecular events underlying the primary response to the Dpp/BMP morphogen in Drosophila. Throughout development, Dpp transduction causes the graded transcriptional downregulation of the brinker (brk) gene. We ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00241-1
更新日期:2003-04-18 00:00:00
abstract::Establishment and maintenance of the blood system relies on self-renewing hematopoietic stem cells (HSCs) that normally reside in small numbers in the bone marrow niche of adult mammals. This Review describes the developmental origins of HSCs and the molecular mechanisms that regulate lineage-specific differentiation....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.01.025
更新日期:2008-02-22 00:00:00
abstract::Two papers published in 1984 by the Varshavsky laboratory revealed that the ubiquitin/proteasome pathway is the principal system for degradation of short-lived proteins in mammalian cells, setting the stage for future demonstrations of this pathway's many regulatory roles. This perspective discusses the impact of thos...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s0092-8674(03)01074-2
更新日期:2004-01-23 00:00:00
abstract::The topology of E. coli inner membrane proteins depends primarily on the distribution of positively charged residues in the molecule. We have constructed model proteins with four potential transmembrane stretches and have systematically explored the topological effects of lysines placed in the loops connecting the tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90155-4
更新日期:1994-05-06 00:00:00
abstract::Excessive accumulation of neurofilaments in the cell bodies and proximal axons of motor neurons is a major pathological hallmark during the early stages of many human motor neuron diseases. To test directly the consequence of overexpression of the major neurofilament subunit NF-L, we produced transgenic mice that accu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90157-l
更新日期:1993-04-09 00:00:00
abstract::The expression of the E2A transcription unit of the adenovirus E1A deletion mutant dl312 in murine embryonal carcinoma (EC) stem cells suggests that they contain an activity that will complement viral E1A. We have prepared from these cells in vitro transcription extracts that use E1A-inducible promoters more efficient...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90453-3
更新日期:1987-05-22 00:00:00
abstract::We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine resi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90038-9
更新日期:1987-08-14 00:00:00
abstract::The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.027
更新日期:2020-10-01 00:00:00
abstract::Yeast heat shock protein 104 (Hsp104), the only known eukaryotic disaggregase, remodels both disordered protein aggregates and cross-β sheet amyloids. To handle this diverse clientele, DeSantis et al. report that Hsp104 hexamers use distinct mechanisms-individual subunits are able to dissolve disordered aggregates, bu...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.10.033
更新日期:2012-11-09 00:00:00
abstract::The global rise in the prevalence of obesity and associated co-morbidities such as type 2 diabetes, cardiovascular disease, and cancer represents a major public health concern. The biological response to increased consumption of palatable foods or a reduction in energy expenditure is highly variable between individual...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2015.03.008
更新日期:2015-03-26 00:00:00
abstract::An approach to identify backbone conformational changes underlying nicotinic acetylcholine receptor (nAChR) gating was developed. Specific backbone peptide bonds were replaced with an ester, which disrupts backbone hydrogen bonds at the site of mutation. At a conserved proline residue (alphaPro221) in the first transm...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80962-9
更新日期:1999-01-08 00:00:00
abstract::Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies, but their subclass composition changed over time, with IgG1 persisting, IgG3 r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.036
更新日期:2019-05-30 00:00:00
abstract::From the approximately 1,200 odorant receptor (OR) genes in the mouse genome, an olfactory sensory neuron is thought to express only one gene. The mechanisms of OR gene choice are not understood. A 2.1 kilobase region (the H element) adjacent to a cluster of seven OR genes has been proposed as a trans- and pan-enhance...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.06.023
更新日期:2007-07-27 00:00:00
abstract::Using a combination of classical genetics, serology, and molecular genetics, we have mapped as many as possible of the MHC components in t haplotypes. Results indicate that the whole MHC is included in a simple inversion with the relative positions of its genes being intact. Furthermore, some of the t-lethal mutations...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90464-1
更新日期:1984-12-01 00:00:00
abstract::We have isolated spontaneous temperature-resistant revertants of a temperature-sensitive mutation (rpoD800) in the sigma subunit of E. coli K12 RNA polymerase. These revertants still contained the rpoD800 allele. They were mucoid, and sensitive to ultraviolet light and the radiomimetic agent nitrofurantoin, which are ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90505-6
更新日期:1983-01-01 00:00:00
abstract::How is the characteristic shape of an organelle generated? Recent work has provided insight into how the tubular network of the endoplasmic reticulum (ER) is formed. The tubules themselves are shaped by the reticulons and DP1/Yop1p, whereas their fusion into a network is brought about by membrane-bound GTPases that in...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.11.022
更新日期:2011-12-09 00:00:00
abstract::A recent comparative analysis of the sequenced genomes of 12 Drosophila species (Drosophila 12 Genomes Consortium, 2007; Stark et al., 2007) reveals a comprehensive picture of the evolution of small animal genomes and greatly improves computational predictions of functional elements in the D. melanogaster reference se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.12.003
更新日期:2007-12-28 00:00:00