Abstract:
:In target tissues for estrogen, including breast cancer cells, the synthesis of progesterone receptors (PRs) is controlled by estradiol acting through estrogen receptors (ERs). We describe studies with T47D human breast cancer cells, whose PRs are not regulated by estradiol, though present in extraordinary amounts (300,000 sites per cell). These cells have no ERs sedimenting at 8S on sucrose density gradients, and no unfilled cytoplasmic or nuclear ERs; some apparently hormone-filled nuclear sites, with KD congruent to 0.7 nM, can be demonstrated by exchange. The nuclear ER sites are not processed after estradiol treatment. Nafoxidine, however, doubles nuclear estrogen binding in 6 hr, in a cycloheximide-insensitive step that may represent a reversal of processing. T47D cells are profoundly resistant to estrogens and antiestrogens; estradiol does not stimulate PRs, and nafoxidine concentrations that are cytotoxic to ER-positive cells have no effect on cell growth or on PR levels. Yet the PRs are normal by several criteria, and they can be stoichiometrically translocated to, and extracted from, nuclei in the first 3 min after progesterone addition. If progesterone treatment exceeds 10 min, rapid nuclear turnover prevents quantitative PR recovery. Cytoplasmic PRs are replenished in 10 to 24 hr, and this cycloheximide-sensitive step is also estrogen- and nafoxidine-resistant. However, despite their insensitivity to estradiol or antiestrogen, PRs are not constitutively synthesized; 5-bromodeoxyuridine and sodium butyrate can selectively inhibit PR production. Thus, since PRs retain some characteristics of inducible proteins, the persistent nuclear estrogen-binding sites may be stimulating PRs continuously, even in the absence of exogenous estradiol.
journal_name
Celljournal_title
Cellauthors
Horwitz KB,Mockus MB,Lessey BAdoi
10.1016/0092-8674(82)90218-5subject
Has Abstractpub_date
1982-03-01 00:00:00pages
633-42issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(82)90218-5journal_volume
28pub_type
杂志文章相关文献
CELL文献大全abstract::Subsets of CD4 T cells are defined by the cytokines that they produce; these cytokines determine the effector function of these cells. Cloned CD4 T cells fall into two subsets, producing either interferon-gamma (IFN gamma) or interleukin-4 (IL-4) in combination with other cytokines, and are called Th1 and Th2 cells, r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90542-x
更新日期:1993-12-03 00:00:00
abstract::How is the characteristic shape of an organelle generated? Recent work has provided insight into how the tubular network of the endoplasmic reticulum (ER) is formed. The tubules themselves are shaped by the reticulons and DP1/Yop1p, whereas their fusion into a network is brought about by membrane-bound GTPases that in...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.11.022
更新日期:2011-12-09 00:00:00
abstract::Trigger factor is a soluble, 63,000 dalton protein of E. coli that stabilizes proOmpA, the precursor form of a major outer-membrane protein, in a conformation competent for in vitro membrane assembly. There is approximately one trigger factor molecule bound to each 70S ribosome isolated from cell extracts in physiolog...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90116-x
更新日期:1988-09-23 00:00:00
abstract::New biological tools provide new techniques to probe fundamental biological processes. Here we describe the burgeoning field of proteolysis-targeting chimeras (PROTACs), which are capable of modulating protein concentrations at a post-translational level by co-opting the ubiquitin-proteasome system. We describe the PR...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.11.031
更新日期:2020-04-02 00:00:00
abstract::Cell-penetrating peptides (CPPs) are short protein segments that can transport cargos into cells. Although CPPs are widely studied as potential drug delivery tools, their role in normal cell physiology is poorly understood. Early during infection, the L2 capsid protein of human papillomaviruses binds retromer, a cytop...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.07.031
更新日期:2018-09-06 00:00:00
abstract::Cytoplasmic dynein is a motor protein that exerts force on microtubules. To generate force for the movement of large organelles, dynein needs to be anchored, with the anchoring sites being typically located at the cell cortex. However, the mechanism by which dyneins target sites where they can generate large collectiv...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.05.020
更新日期:2013-06-20 00:00:00
abstract::In this issue, Marcandalli et al. (2019) report a self-assembling nanoparticle bearing an antigen from respiratory syncytial virus. This is the first time the structure, stability, and adjuvanticity of an antigen have been rationally designed at the atomic level and incorporated in one vaccine. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.02.008
更新日期:2019-03-07 00:00:00
abstract::Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90037-0
更新日期:1984-04-01 00:00:00
abstract::Reactive oxygen species (ROS) and mitochondrial defects in neurons are implicated in neurodegenerative disease. Here, we find that a key consequence of ROS and neuronal mitochondrial dysfunction is the accumulation of lipid droplets (LD) in glia. In Drosophila, ROS triggers c-Jun-N-terminal Kinase (JNK) and Sterol Reg...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.12.019
更新日期:2015-01-15 00:00:00
abstract::We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81164-2
更新日期:1998-05-01 00:00:00
abstract::MicroRNAs are small endogenous noncoding RNAs involved in posttranscriptional gene regulation. During microRNA biogenesis, Drosha and Dicer process the primary transcript (pri-miRNA) through a precursor hairpin (pre-miRNA) to the mature miRNA. The miRNA is incorporated into the RNA-Induced Silencing Complex (RISC) wit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.10.032
更新日期:2007-12-14 00:00:00
abstract::X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90379-9
更新日期:1984-06-01 00:00:00
abstract::The brightness of our visual environment varies tremendously from day to night. In this issue of Cell, Milner and Do describe how the population of retinal neurons responsible for entrainment of the brain's circadian clock cooperate to encode irradiance across a wide range of ambient-light intensities. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.10.028
更新日期:2017-11-02 00:00:00
abstract::We have shown that covalent conjugation of ubiquitin to proteins is temperature-sensitive in the mouse cell cycle mutant ts85 due to a specifically thermolabile ubiquitin-activating enzyme (accompanying paper). We show here that degradation of short-lived proteins is also temperature sensitive in ts85 , in contrast to...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90300-3
更新日期:1984-05-01 00:00:00
abstract::The mec-3 gene is essential for proper differentiation of the set of six touch receptor neurons in C. elegans. In mutants lacking mec-3 activity, the touch receptors express none of their unique differentiated features and appear to be transformed into other types of neurons. We cloned the mec-3 gene by transposon tag...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90174-2
更新日期:1988-07-01 00:00:00
abstract::Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90073-2
更新日期:1975-09-01 00:00:00
abstract::We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structura...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90395-7
更新日期:1984-06-01 00:00:00
abstract::T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the capability of CAR T cells, here, we present a split, universal, and programmable (SUPRA) CAR system that simultaneously encompasses multiple ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.03.038
更新日期:2018-05-31 00:00:00
abstract::Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90018-5
更新日期:1975-06-01 00:00:00
abstract::To mechanistically characterize the microevolutionary processes active in altering transcription factor (TF) binding among closely related mammals, we compared the genome-wide binding of three tissue-specific TFs that control liver gene expression in six rodents. Despite an overall fast turnover of TF binding location...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.07.007
更新日期:2013-08-01 00:00:00
abstract::Thymocyte commitment to the CD4 helper versus CD8 cytotoxic lineage has not been satisfactorily established. Two models have been elaborated: one based on instruction, the other on selection. Most previous results support the instructive model, but our comparison of thymocyte differentiation in MHC class II-, class I-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90225-f
更新日期:1993-04-23 00:00:00
abstract::Phosphoinositide 3 kinase (PI3K) is a key signaling enzyme implicated in receptor-stimulated mitogenesis, oxidative bursting in neutrophils, membrane ruffling, and glucose uptake. A PI3K has already been purified, cloned, and shown to be regulated by receptors that act via tyrosine kinase-dependent regulatory mechanis...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90237-2
更新日期:1994-04-08 00:00:00
abstract::Plasmids expressing partial-length sense or antisense protein 4.1 RNA were microinjected into fertilized Xenopus eggs. Nuclease protection assays reveal that antisense protein 4.1 RNA lead to the specific loss of endogenous protein 4.1 transcripts after midblastula transition, with no effect on the levels of three unr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90576-4
更新日期:1988-05-20 00:00:00
abstract::A key aspect of genomic medicine is to make individualized clinical decisions from personal genomes. We developed a machine-learning framework to integrate personal genomes and electronic health record (EHR) data and used this framework to study abdominal aortic aneurysm (AAA), a prevalent irreversible cardiovascular ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.07.021
更新日期:2018-09-06 00:00:00
abstract::Much like the "microbe hypothesis" put forth over 150 years ago, the "prion hypothesis" can be definitely proven only if a prion disease is engendered in a natural host from an infectious prion produced in vitro. In this issue of Cell, come very close to accomplishing this goal by producing a prion disease in a natura...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2005.04.002
更新日期:2005-04-22 00:00:00
abstract::The murine major histocompatibility complex I region encodes two class II antigens, I-A and I-E. From a mouse spleen DNA cosmid library of the b haplotype, we isolated a clone containing the entire I-A beta gene and a separate exon encoding a beta-chain second domain (A beta 2). The A beta gene, encompassing more than...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90148-4
更新日期:1983-08-01 00:00:00
abstract::We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90026-8
更新日期:1988-11-04 00:00:00
abstract::Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 def...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.036
更新日期:2012-05-11 00:00:00
abstract::The genetics of complex disease produce alterations in the molecular interactions of cellular pathways whose collective effect may become clear through the organized structure of molecular networks. To characterize molecular systems associated with late-onset Alzheimer's disease (LOAD), we constructed gene-regulatory ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.03.030
更新日期:2013-04-25 00:00:00
abstract::New neurons arise from quiescent adult neural progenitors throughout life in specific regions of the mammalian brain. Little is known about the embryonic origin and establishment of adult neural progenitors. Here, we show that Hopx+ precursors in the mouse dentate neuroepithelium at embryonic day 11.5 give rise to pro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.02.010
更新日期:2019-04-18 00:00:00