Abstract:
:Trigger factor is a soluble, 63,000 dalton protein of E. coli that stabilizes proOmpA, the precursor form of a major outer-membrane protein, in a conformation competent for in vitro membrane assembly. There is approximately one trigger factor molecule bound to each 70S ribosome isolated from cell extracts in physiological buffers. Trigger factor dissociates from ribosomes in 1.5 M LiCl and reassociates with salt-washed ribosomes in low-salt buffer. Binding is exclusively to the 50S (large) subunit, known to contain the exit domain for nascent polypeptide chains. In addition to its associations with proOmpA and ribosomes, excess trigger factor can compete with the proOmpA-trigger factor complex for a limited number of membrane sites that are essential for translocation of proOmpA. These data suggest a model of trigger factor cycling between the cytoplasm, the ribosome, presecretory proteins, and membrane receptor proteins.
journal_name
Celljournal_title
Cellauthors
Lill R,Crooke E,Guthrie B,Wickner Wdoi
10.1016/0092-8674(88)90116-xsubject
Has Abstractpub_date
1988-09-23 00:00:00pages
1013-8issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(88)90116-Xjournal_volume
54pub_type
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