Abstract:
:In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. To achieve these normally opposing goals, we developed grazing incidence structured illumination microscopy (GI-SIM) that is capable of imaging dynamic events near the basal cell cortex at 97-nm resolution and 266 frames/s over thousands of time points. We employed multi-color GI-SIM to characterize the fast dynamic interactions of diverse organelles and the cytoskeleton, shedding new light on the complex behaviors of these structures. Precise measurements of microtubule growth or shrinkage events helped distinguish among models of microtubule dynamic instability. Analysis of endoplasmic reticulum (ER) interactions with other organelles or microtubules uncovered new ER remodeling mechanisms, such as hitchhiking of the ER on motile organelles. Finally, ER-mitochondria contact sites were found to promote both mitochondrial fission and fusion.
journal_name
Celljournal_title
Cellauthors
Guo Y,Li D,Zhang S,Yang Y,Liu JJ,Wang X,Liu C,Milkie DE,Moore RP,Tulu US,Kiehart DP,Hu J,Lippincott-Schwartz J,Betzig E,Li Ddoi
10.1016/j.cell.2018.09.057subject
Has Abstractpub_date
2018-11-15 00:00:00pages
1430-1442.e17issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(18)31308-4journal_volume
175pub_type
杂志文章相关文献
CELL文献大全abstract::To defend cells against viruses, the MAVS (mitochondrial antiviral signaling) adaptor protein initiates an antiviral signaling cascade from mitochondrial membranes. In this issue, Dixit et al. (2010) show that MAVS also localizes to the membranes of peroxisomes, where it rapidly induces expression of a subset of antiv...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.04.043
更新日期:2010-05-14 00:00:00
abstract::CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at a hi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80240-8
更新日期:1997-05-16 00:00:00
abstract::In this issue of Cell, Korber et al. found that a SARS-CoV-2 variant in the spike protein D614G rapidly became dominant around the world. Although clinical and in vitro data suggest that D614G changes the virus phenotype, the impact of the mutation on transmission, disease, and vaccine and therapeutic development are ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2020.06.040
更新日期:2020-08-20 00:00:00
abstract::At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.023
更新日期:2012-04-13 00:00:00
abstract::Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.03.004
更新日期:2013-03-28 00:00:00
abstract::Sleep is characterized by behavioral quiescence, homeostasis, increased arousal threshold, and rapid reversibility. Understanding how these properties are encoded by a neuronal circuit has been difficult, and no single molecular or neuronal pathway has been shown to be responsible for the regulation of sleep. Taking a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.11.036
更新日期:2014-01-16 00:00:00
abstract::A key function of blood vessels, to supply oxygen, is impaired in tumors because of abnormalities in their endothelial lining. PHD proteins serve as oxygen sensors and may regulate oxygen delivery. We therefore studied the role of endothelial PHD2 in vessel shaping by implanting tumors in PHD2(+/-) mice. Haplodeficien...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.01.020
更新日期:2009-03-06 00:00:00
abstract::The mitochondrial unfolded protein response (UPRmt) can be triggered in a cell-non-autonomous fashion across multiple tissues in response to mitochondrial dysfunction. The ability to communicate information about the presence of mitochondrial stress enables a global response that can ultimately better protect an organ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.06.029
更新日期:2018-08-09 00:00:00
abstract::Three different cases of pancreatic beta cell hyperplasia in mice are accompanied by an increase in a subclass of cells expressing tyrosine hydroxylase (TH), a neuronal enzyme. In the nontumorigenic cases of islet growth during normal pregnancy and in the obese mutant mouse, the TH-insulin cells do not divide, in cont...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90534-x
更新日期:1988-01-15 00:00:00
abstract::The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, transcription. Here we describe the isolation and characterization of the first gene en...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90664-c
更新日期:1993-01-29 00:00:00
abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90221-5
更新日期:1982-03-01 00:00:00
abstract::Maintaining the pluripotency of mouse ES cells requires both LIF (leukemia inhibitory factor) and unknown factors in serum. The paper from Ying et al. in this issue of Cell shows that BMP (bone morphogenetic protein) can replace serum in this capacity, defining molecular requirements for ES cell self-renewal. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/s0092-8674(03)00853-5
更新日期:2003-10-31 00:00:00
abstract::Bacteria have evolved many different means of generating movement. In this issue of Cell, Shaevitz et al. (2005) describe the swimming movement of a helical bacterium called Spiroplasma. They discover that Spiroplasma propels itself by generating two temporally distinct kinks that travel the length of the bacterium. T...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2005.09.003
更新日期:2005-09-23 00:00:00
abstract:SUMMARY:Disruption of the cyclin-dependent kinase-inhibitory domain of p27 enhances growth of mice. Growth is attributed to an increase in cell number, due to increased cell proliferation, most obviously in tissues that ordinarily express p27 at the highest levels. Disruption of p27 function leads to nodular hyperplasi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81238-6
更新日期:1996-05-31 00:00:00
abstract::Trigger factor (TF) is a molecular chaperone that binds to bacterial ribosomes where it contacts emerging nascent chains, but TF is also abundant free in the cytosol where its activity is less well characterized. In vitro studies show that TF promotes protein refolding. We find here that ribosome-free TF stably associ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.07.044
更新日期:2009-09-04 00:00:00
abstract::DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90446-j
更新日期:1992-05-01 00:00:00
abstract::Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To deter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90846-5
更新日期:1989-07-28 00:00:00
abstract::Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.050
更新日期:2012-01-20 00:00:00
abstract::We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90290-2
更新日期:1982-10-01 00:00:00
abstract::The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 A resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making exte...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00272-0
更新日期:2001-03-09 00:00:00
abstract::NRK fibroblasts exposed to transforming growth factor-beta (TGF-beta) show increased binding of radiolabeled epidermal growth factor (EGF) relative to untreated cells. The binding of another growth factor, rat insulin-like growth factor-II, is unaffected. The increase in EGF binding induced by TGF-beta is not due to i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90071-0
更新日期:1984-01-01 00:00:00
abstract::Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM Na...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90188-x
更新日期:1977-09-01 00:00:00
abstract::Mutant strains of D. discoideum are described that can complete morphogenesis and cytodifferentiation but which express vastly reduced levels of the galactose-binding lectins discoidin I and II (less than 1% and 1%-2% respectively) compared to the wild-type control. Mutant cells proceeding through development lack lec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90380-x
更新日期:1983-09-01 00:00:00
abstract::Forms of RNA polymerase I prepared from growing or encysted Acanthamoeba are equal in the ability to transcribe poly(dl:dC). Polymerase from cysts, whose rRNA genes are transcriptionally inactive, is unable to utilize the rDNA promoter in vitro, whereas the transcription initiation factor from cysts is fully able to b...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90601-x
更新日期:1986-11-07 00:00:00
abstract::Eukaryotic genomes are packaged into nucleosomes whose position and chemical modification state can profoundly influence regulation of gene expression. We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.06.026
更新日期:2005-08-26 00:00:00
abstract::The primer transcript of plasmid ColE1 extends beyond the replication origin in either of two different modes. It does or does not form a hybrid with the template DNA. When a stretch of 20 deoxyadenosine residues is inserted into the template strand downstream of the origin, more than 90% of hybridized transcripts and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90131-0
更新日期:1987-11-20 00:00:00
abstract::Many naturalistic behaviors are built from modular components that are expressed sequentially. Although striatal circuits have been implicated in action selection and implementation, the neural mechanisms that compose behavior in unrestrained animals are not well understood. Here, we record bulk and cellular neural ac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.04.019
更新日期:2018-06-28 00:00:00
abstract::We have shown that the DNA sequences adjacent to the telomeres of Saccharomyces cerevisiae chromosomes are highly conserved and contain a high density of replication origins. The salient features of these telomeres can be summarized as follows. There are three moderately repetitive elements present at the telomeres: t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90437-3
更新日期:1983-06-01 00:00:00
abstract::Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80051-9
更新日期:1985-07-01 00:00:00
abstract::The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90150-b
更新日期:1992-02-21 00:00:00