Abstract：
:Forms of RNA polymerase I prepared from growing or encysted Acanthamoeba are equal in the ability to transcribe poly(dl:dC). Polymerase from cysts, whose rRNA genes are transcriptionally inactive, is unable to utilize the rDNA promoter in vitro, whereas the transcription initiation factor from cysts is fully able to bind the promoter and direct transcription. Footprinting shows that polymerase from cysts is functionally inactive because of its inability to bind to the promoter. The polymerase footprint moves downstream the appropriate number of base pairs upon various nucleotide additions, without affecting the factor footprint. These results support our hypothesis that rRNA synthesis in eukaryotes is regulated by polymerase I modification and not by alterations to additional DNA-binding proteins.
journal_name
Celljournal_title
Cellauthors
Bateman E,Paule MRdoi
10.1016/0092-8674(86)90601-xsubject
Has Abstractpub_date
1986-11-07 00:00:00pages
445-50issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(86)90601-Xjournal_volume
47pub_type
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