Abstract：
:Cytotoxic T lymphocytes and lymphocytes with NK-like activity contain a serine esterase activity which has been localized to their cytoplasmic granules by cytochemistry and subcellular fractionation studies. The serine esterase-specific inhibitor 3H-DFP labels two protein species in the granules. The two proteins, referred to as serine esterases 1 and 2 (SE 1 and SE 2), migrate with Mr of 34-36 kd and 28-30 kd, respectively, under reducing conditions. SE 1 shows trypsin-like activity and has been purified to apparent homogeneity. Under nonreducing conditions, SE 1 has an Mr of 60-66 kd, suggesting that it may consist of two disulfide-linked subunits of 34-36 kd each. SE 1 cleaves fibrin and casein, has a pl greater than 10, and optimal activity at pH 8. The substrate specificity of SE 2 is not known. The serine esterase activity is secreted by lymphocytes that have been stimulated with the calcium ionophore A23187. The serine esterases described here could play an active role in cell-mediated killing.
journal_name
Celljournal_title
Cellauthors
Young JD,Leong LG,Liu CC,Damiano A,Wall DA,Cohn ZAdoi
10.1016/0092-8674(86)90441-1subject
Has Abstractpub_date
1986-10-24 00:00:00pages
183-94issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(86)90441-1journal_volume
47pub_type
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