Abstract：
:Mutants in bacterial topoisomerase (topo) IV are deficient in chromosomal partitioning. To investigate the basis of this phenotype, we examined plasmid DNA topology in conditionally lethal topo IV mutants. We found that dimeric catenated plasmids accumulated in vivo after topo IV inhibition. The catenanes were supercoiled, contained from 2 to > 32 nodes, and were the products of DNA synthesis. Electron microscopy and recombination tests proved that the catenanes have the unique structure predicted for replication intermediates. These data provide strong evidence for a model in which unlinking of the double helix can occur in two stages during DNA replication and for the critical role of topo IV in the second stage. The interlocks in the catenanes appear to be sequestered from DNA gyrase, perhaps by compartmentalization in an enzyme complex dedicated to partitioning.
journal_name
Celljournal_title
Cellauthors
Adams DE,Shekhtman EM,Zechiedrich EL,Schmid MB,Cozzarelli NRdoi
10.1016/0092-8674(92)90356-hsubject
Has Abstractpub_date
1992-10-16 00:00:00pages
277-88issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(92)90356-Hjournal_volume
71pub_type
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