Abstract：
:We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an inability to retrieve synaptic membrane. This defect is accompanied by an expansion of the presynaptic membrane, and a depletion of vesicles from the bouton lumen. Interestingly, mutant larvae are still able to sustain release at 15%-20% of the normal rate during high-frequency stimulation. We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals.
journal_name
Celljournal_title
Cellauthors
Verstreken P,Kjaerulff O,Lloyd TE,Atkinson R,Zhou Y,Meinertzhagen IA,Bellen HJdoi
10.1016/s0092-8674(02)00688-8subject
Has Abstractpub_date
2002-04-05 00:00:00pages
101-12issue
1eissn
0092-8674issn
1097-4172pii
S0092867402006888journal_volume
109pub_type
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