Abstract：
:We describe the comprehensive characterization of homeodomain DNA-binding specificities from a metazoan genome. The analysis of all 84 independent homeodomains from D. melanogaster reveals the breadth of DNA sequences that can be specified by this recognition motif. The majority of these factors can be organized into 11 different specificity groups, where the preferred recognition sequence between these groups can differ at up to four of the six core recognition positions. Analysis of the recognition motifs within these groups led to a catalog of common specificity determinants that may cooperate or compete to define the binding site preference. With these recognition principles, a homeodomain can be reengineered to create factors where its specificity is altered at the majority of recognition positions. This resource also allows prediction of homeodomain specificities from other organisms, which is demonstrated by the prediction and analysis of human homeodomain specificities.
journal_name
Celljournal_title
Cellauthors
Noyes MB,Christensen RG,Wakabayashi A,Stormo GD,Brodsky MH,Wolfe SAdoi
10.1016/j.cell.2008.05.023subject
Has Abstractpub_date
2008-06-27 00:00:00pages
1277-89issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(08)00682-Xjournal_volume
133pub_type
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