Abstract:
:Membrane subdomains have been implicated in T cell signaling, although their properties and mechanisms of formation remain controversial. Here, we have used single-molecule and scanning confocal imaging to characterize the behavior of GFP-tagged signaling proteins in Jurkat T cells. We show that the coreceptor CD2, the adaptor protein LAT, and tyrosine kinase Lck cocluster in discrete microdomains in the plasma membrane of signaling T cells. These microdomains require protein-protein interactions mediated through phosphorylation of LAT and are not maintained by interactions with actin or lipid rafts. Using a two color imaging approach that allows tracking of single molecules relative to the CD2/LAT/Lck clusters, we demonstrate that these microdomains exclude and limit the free diffusion of molecules in the membrane but also can trap and immobilize specific proteins. Our data suggest that diffusional trapping through protein-protein interactions creates microdomains that concentrate or exclude cell surface proteins to facilitate T cell signaling.
journal_name
Celljournal_title
Cellauthors
Douglass AD,Vale RDdoi
10.1016/j.cell.2005.04.009subject
Has Abstractpub_date
2005-06-17 00:00:00pages
937-50issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(05)00352-1journal_volume
121pub_type
杂志文章相关文献
CELL文献大全abstract::The X-linked Foxp3 is a member of the forkhead/winged helix transcription factor family. Germline mutations cause lethal autoimmune diseases in males. Serendipitously, we observed that female mice heterozygous for the "scurfin" mutation of the Foxp3 gene (Foxp3(sf/+)) developed cancer at a high rate. The majority of t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.04.034
更新日期:2007-06-29 00:00:00
abstract::22q11.2 microdeletions result in specific cognitive deficits and schizophrenia. Analysis of Df(16)A(+/-) mice, which model this microdeletion, revealed abnormalities in the formation of neuronal dendrites and spines, as well as altered brain microRNAs. Here, we show a drastic reduction of miR-185, which resides within...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.11.052
更新日期:2013-01-17 00:00:00
abstract::Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90382-o
更新日期:1990-09-21 00:00:00
abstract::The 2013-2015 West African epidemic of Ebola virus disease (EVD) reminds us of how little is known about biosafety level 4 viruses. Like Ebola virus, Lassa virus (LASV) can cause hemorrhagic fever with high case fatality rates. We generated a genomic catalog of almost 200 LASV sequences from clinical and rodent reserv...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.07.020
更新日期:2015-08-13 00:00:00
abstract::Mitochondrial DNA from bovine tissue contains heterogeneous sequences located within an evolutionary conserved cytosine homopolymer sequence near the 5' end of the D-loop region. This part of the mammalian mitochondrial genome is known to contain the origin of heavy strand DNA synthesis and the major transcriptional p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90434-3
更新日期:1984-07-01 00:00:00
abstract::The science advisor to the US president has the opportunity to influence how scientific research is supported by the federal government, as well as the ways in which science and technology can be applied to meet the needs of society. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.11.024
更新日期:2009-11-25 00:00:00
abstract::c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90663-b
更新日期:1993-01-29 00:00:00
abstract::Yeast ataxin-2, also known as Pbp1 (polyA binding protein-binding protein 1), is an intrinsically disordered protein implicated in stress granule formation, RNA biology, and neurodegenerative disease. To understand the endogenous function of this protein, we identify Pbp1 as a dedicated regulator of TORC1 signaling an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.02.043
更新日期:2019-04-18 00:00:00
abstract::Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90252-6
更新日期:1994-11-04 00:00:00
abstract::Negative selection with FUdR produced revertants from the transformed rat line 14B, which contains one insertion of the SV40 viral genome (Botchan, Topp and Sambrook, 1976). 14B contains nuclear T antigen, grows to a high density, grows in low serum and is anchorage-independent. The revertants fall into three classes ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90134-4
更新日期:1978-01-01 00:00:00
abstract::Childhood asthma is typically associated with a polarized Th2 response to inhaled allergens and is influenced by genetics. Yang et al. show that the asthma susceptibility gene DENND1B controls cytokine production in Th2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes, providing a mo...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.12.045
更新日期:2016-01-14 00:00:00
abstract::The complete sequence of a hemagglutinin (HA) gene of a recent human influenza A strain, A/Victoria/3/75, is 1768 nucleotides long and contains the information for 567 amino acids. It codes for a signal peptide of 16 amino acids, the HA1 chain of the mature hemagglutinin of 329 amino acids, a connecting region between...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(80)80045-6
更新日期:1980-03-01 00:00:00
abstract::Transfer of human chromosome 11, which contains the myoD locus, from primary fibroblasts into 10T1/2 cells results in activation of myoD. In contrast, hybrids that retain human chromosome 11 and additional human chromosomes fail to activate myoD. We show that human chromosome 4 inhibits myoD activation. myoD enhancer/...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90033-0
更新日期:1995-08-25 00:00:00
abstract::The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal "cap" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-l...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90022-2
更新日期:1976-02-01 00:00:00
abstract::Although specific microRNAs (miRNAs) can be upregulated in cancer, global miRNA downregulation is a common trait of human malignancies. The mechanisms of this phenomenon and the advantages it affords remain poorly understood. Here we identify a microRNA family, miR-103/107, that attenuates miRNA biosynthesis by target...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.05.017
更新日期:2010-06-25 00:00:00
abstract::During RNA interference (RNAi), Dicer generates short interfering RNAs (siRNAs), which then guide target mRNA cleavage by the RISC complex. Now, Liu et al. identify R2D2, a Dicer-associated protein that is important for siRNA incorporation into RISC, thus linking the initiation and execution phases of RNAi. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00806-7
更新日期:2003-10-17 00:00:00
abstract::In Drosophila melanogaster males, the sex chromosomes pair during meiosis in the centric X heterochromatin and at the base of the short arm of the Y (YS), in the vicinity of the nucleolus organizers. X chromosomes deficient for the pairing region segregate randomly from the Y. In this report we show that a single ribo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90215-z
更新日期:1990-04-06 00:00:00
abstract::An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90379-3
更新日期:1983-09-01 00:00:00
abstract::The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.04.006
更新日期:2015-04-23 00:00:00
abstract::The role of the noncoding genome in cancer biology is continually expanding. Cho et al. reveal a new and unexpected mechanism for the regulation of MYC expression mediated by the promoter sequence of its neighbor gene PVT1. This DNA element acts as a promoter-enhancer competitor and a candidate tumor suppressor. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2018.05.023
更新日期:2018-05-31 00:00:00
abstract::The enteric bacteria Salmonella typhimurium has the ability to invade (enter) nonphagocytic cells. The internalization process occurs as a result of an intimate interaction between the bacteria and the host cell, in which S. typhimurium triggers a cascade of host cell-signaling events leading to the formation of host ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90510-x
更新日期:1994-02-25 00:00:00
abstract::The Xist gene maps to the X inactivation center region in both mouse and human, and previous analysis of the 3' end of the gene has demonstrated inactive X-specific expression, suggesting a possible role in X inactivation. We have now analyzed the entire mouse Xist gene. The mature inactive X-specific transcript is 15...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90519-i
更新日期:1992-10-30 00:00:00
abstract::While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90753-8
更新日期:1989-12-22 00:00:00
abstract::Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81113-7
更新日期:1996-05-03 00:00:00
abstract::The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does no...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90808-r
更新日期:1990-04-20 00:00:00
abstract::The non-receptor protein tyrosine phosphatase (PTP) SHP2, encoded by PTPN11, plays an essential role in RAS-mitogen-activated protein kinase (MAPK) signaling during normal development. It has been perplexing as to why both enzymatically activating and inactivating mutations in PTPN11 result in human developmental diso...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.002
更新日期:2020-10-15 00:00:00
abstract::Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81742-0
更新日期:1998-09-18 00:00:00
abstract::M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90099-4
更新日期:1981-06-01 00:00:00
abstract::Dopamine plays an important role in the etiology of schizophrenia, and D2 class dopamine receptors are the best-established target of antipsychotic drugs. Here we show that D2 class-receptor-mediated Akt regulation involves the formation of signaling complexes containing beta-arrestin 2, PP2A, and Akt. beta-arrestin 2...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.05.012
更新日期:2005-07-29 00:00:00
abstract::Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.12.044
更新日期:2006-03-24 00:00:00